Differences in antibody production against mouse hepatitis virus (MHV) among mouse strains

Abstract

Several strains of mice were examined for antibody production after intranasal inoculation with a low virulence strain of mouse hepatitis virus (MHV), MHV-NuU. C57BL/6N mice were shown to be high responders in the production of complement fixing (CF) antibody as compared to C3H/HeN, BALB/c-AnN, DBA/2N mice. F1 hybrids B6C3 and BDF1 from C57BL/6N mice, showed CF antibody responses as high as C57BL/6N, suggesting that high responsiveness is genetically controlled. All these mouse strains were able to produce high titred neutralizing antibody to MHV.

Characterization of Low‐virulent Mouse Coronavirus Isolated from Faeces in a Mouse Colony

“…Since many MHV strains passaged in DBT cells have been studied for their pathogenicity in 4-week-old ICR mice (10,13,15,16,24), we also examined the pathogenicity of these two possible enterotropic strains in the same mouse strain. As a control, a well characterized low-virulent strain, MHV-NuU (16,21,25,26), was included in the experimental infection. All the mice inoculated with H C A or each virus strain alone survived for 14 days, and the liver was apparently normal at necropsy.…”

“…Serology: Serum neutralization test was carried out by the plaque reduction method (13,21), and immunofluorescence was performed by the indirect method (17). Antisera for the serum neutralization test were prepared in rabbits (13) or mice (21). Anti-JHM rabbit serum and FITC-conjugated anti-rabbit IgG goat serum (Cappel Lab.)…”

Viral Infections of Laboratory Mice

“…Multiplex fluorescent immunoassay, ELISA and IFA are well established and sensitive, and all known MHV strains cross-react in these tests [301,350,351]. The magnitude of antibody response depends on MHV strain and mouse genotype [319,352]. DBA/2 mice are poor antibody responders whereas C57BL/6 mice produce a high antibody titre and are therefore good sentinels.…”