Differences in betaine lipids and fatty acids between Pseudoisochrysis paradoxa VLP and Diacronema vlkianum VLP isolates (Haptophyta)

Armada, Isabel; Hachero‐Cruzado, Ismael; Mazuelos, Narciso; Rı́os, José Luis; Manchado, Manuel; Cañavate, José Pedro

doi:10.1016/j.phytochem.2013.07.024

Cited by 32 publications

(21 citation statements)

References 55 publications

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“…This drop in phosphatase activity at high DIP concentrations suggests a higher capability of growing at low P conditions for Isochrysis compared to Nannochloris which could be explained by a lower P demand and/or an unknown effect of the internal P storage on the enzymatic regulation and P uptake of each species. The possibility of a lower P demand is supported by the recent biochemical identification of betaine lipids replacing phospholipids almost completely in these two haptophytes isolated from the same study site Armada et al 2013). Same as phospholipids, betaine lipids are polar glycerolipids, but with no phosphorus or carbohydrate groups.…”

Section: Discussionmentioning

confidence: 90%

“…These bloom-forming microalgae were not toxic or harmful to fish, quite the opposite: Cultures of the genus Isochrysis are considered a high-quality food for fish and shrimp because they exhibit a large concentration of unsaturated fatty acids (van Bergeijk et al 2010). These two strains of haptophytes were isolated from the study fish ponds and cultured in the laboratory by Armada et al (2013): After initial observations of pure cultures under the microscope, the isolates were tentatively assigned to the genus Isochrysis and Diacronema though they were later identified as Pseudoisochrysis paradoxa VLP and Diacronema vlkianum VLP based on their SSU rDNA homology to other haptophytes (Armada et al 2013). In the present study, we have opted for a more conservative nomenclature (Isochrysis sp., and Diacronema sp., respectively) as long as the haptophyte taxonomy is clarified because Pseudoisochrysis paradoxa has been recently reported to be a nomen nudum as it is morphologically and genetically identical to Isochrysis galbana according to Bendif et al (2013).…”

Section: Discussionmentioning

confidence: 99%

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Bloom-Forming Microalgae in High-Species Phytoplankton Assemblages Under Light-Fluctuating and Low Phosphate Conditions

Fernández-Rodríguez

Hidalgo-Lara

Jiménez‐Rodríguez

et al. 2014

Estuaries and Coasts

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Wide changes of key environmental conditions prevented competitive exclusion in the phytoplankton assemblage of a turbid shallow wetland complex artificially managed for aquaculture production (Doñana Natural Park, SW Spain), and some genera showed specific adaptations to exploit those changes. The seasonal pattern of light, temperature and conductivity was frequently overridden by irregular events of sediment resuspension. Consequently, these shallow aquaculture ponds exhibited wide fluctuations of underwater light climate with clear-water, inorganic-turbid and phytoplankton-turbid phases that accommodated a variety of primary producers, such as submerged macrophytes, a softmud bottom phytobenthos and a phytoplankton assemblage of 72 recorded taxa. The algal biomass in the benthos was about 10 times higher than that found in the plankton (as estimated by chlorophyll a concentration) though the phytoplankton bloomed (>10 6 cell ml −1 ) on 10 occasions with four microalgae: Isochrysis sp. (Haptophyta), Diacronema sp. (Haptophyta), Nannochloris sp. (Chlorophyta) and Nephroselmis pyriformis (Chlorophyta). Several environmental conditions influenced microalgae both concurrently and in anticipation (with a time lag of 8-18 days), and this influence was different for both haptophytes with regard to Nannochloris sp. which significantly thrived when the water conductivity was above 12 mS cm −1 (about 6.85 psu of salinity) and the water dissolved inorganic phosphate (DIP) concentration was >0.40 μM. In contrast, the density of Isochrysis sp. was significantly higher at a water DIP concentration <0.40 μM, while the density of Diacronema sp. was higher when the pore water DIP concentration was above 4 μg g −1 d.w., suggesting that each microalgae bloomed at different conditions of P availability.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Bloom-Forming Microalgae in High-Species Phytoplankton Assemblages Under Light-Fluctuating and Low Phosphate Conditions

Fernández-Rodríguez

Hidalgo-Lara

Jiménez‐Rodríguez

et al. 2014

Estuaries and Coasts

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“…DGTA has the same mass as diacylglyceryl-N,N,N-trimethylhomoserine (DGTS) but could be discriminated by a different migration position on two-dimensional TLC (Vogel and Eichenberger, 1992) and, following tandem mass spectrometry (MS2) fragmentation, by the absence of a fragment corresponding to a loss of mass-to-charge ratio (m/z 87; Armada et al, 2013). We did not detect the presence of DGTS in P. tricornutum.…”

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confidence: 73%

Membrane Glycerolipid Remodeling Triggered by Nitrogen and Phosphorus Starvation inPhaeodactylum tricornutum

et al. 2014

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Diatoms constitute a major phylum of phytoplankton biodiversity in ocean water and freshwater ecosystems. They are known to respond to some chemical variations of the environment by the accumulation of triacylglycerol, but the relative changes occurring in membrane glycerolipids have not yet been studied. Our goal was first to define a reference for the glycerolipidome of the marine model diatom Phaeodactylum tricornutum, a necessary prerequisite to characterize and dissect the lipid metabolic routes that are orchestrated and regulated to build up each subcellular membrane compartment. By combining multiple analytical techniques, we determined the glycerolipid profile of P. tricornutum grown with various levels of nitrogen or phosphorus supplies. In different P. tricornutum accessions collected worldwide, a deprivation of either nutrient triggered an accumulation of triacylglycerol, but with different time scales and magnitudes. We investigated in depth the effect of nutrient starvation on the Pt1 strain (Culture Collection of Algae and Protozoa no. 1055/3). Nitrogen deprivation was the more severe stress, triggering thylakoid senescence and growth arrest. By contrast, phosphorus deprivation induced a stepwise adaptive response. The time scale of the glycerolipidome changes and the comparison with large-scale transcriptome studies were consistent with an exhaustion of unknown primary phosphorusstorage molecules (possibly polyphosphate) and a transcriptional control of some genes coding for specific lipid synthesis enzymes. We propose that phospholipids are secondary phosphorus-storage molecules broken down upon phosphorus deprivation, while nonphosphorus lipids are synthesized consistently with a phosphatidylglycerol-to-sulfolipid and a phosphatidycholine-to-betaine lipid replacement followed by a late accumulation of triacylglycerol.

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“…The presence of betaine lipids (BL) among eukaryotic phytoplankton (Dembitsky, 1996) represents additional lipid classes specifically adapted to replace PL in low-P environments that cannot be found in higher plants. This richer lipid diversity is becoming better elucidated (Armada et al, 2013;Banskota et al, 2013;Leblond et al, 2013;Anesi & Guella, 2015;Cañavate et al, 2016) and suggests that the microalgal lipid profile could be even more plastic than that of higher plants under the influence of P nutrition. However, significant research advances are still needed to determine the extent of microalgal differentiation compared with higher plants in the lipid response to P availability (Boudi ere et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Interspecific variability in phosphorus‐induced lipid remodelling among marine eukaryotic phytoplankton

2016

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The response of marine microalgal lipids to phosphorus is of central importance in phytoplankton ecology but remains poorly understood. We determined how taxonomically diverse microalgal species remodelled their lipid class profile in response to phosphorus availability and whether these changes coincided with those already known to occur in land plants and in the limited number of phytoplankton species for which data are available. The complete lipid class profile and specific lipid ratios influenced by phosphorus availability were quantified in two green microalgae and seven Chromalveolates exposed to phosphorus repletion, deprivation and replenishment. Lipid class cell quota changes in the two green microalgae resembled the currently described pattern of betaine lipids substituting for phospholipids under phosphorus depletion, whereas only two of the studied Chromalveolates showed this pattern. Sulpholipids counterbalanced phosphatidylglycerol only in Picochlorum atomus. In all other species, both lipids decreased simultaneously under phosphorus deprivation, although sulpholipids declined more slowly. Phosphorus deprivation always induced a decrease in digalactosyl-diacylglycerol. However, the ratio of digalactosyl-diacylglycerol to total phospholipids increased in eight species and remained unchanged in Isochrysis galbana. Marine phytoplankton seems to have evolved a diversified mechanism for remodelling its lipid class profile under the influence of phosphorus, with cryptophytes and particularly haptophytes exhibiting previously unobserved lipid responses to phosphorus.

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Differences in betaine lipids and fatty acids between Pseudoisochrysis paradoxa VLP and Diacronema vlkianum VLP isolates (Haptophyta)

Cited by 32 publications

References 55 publications

Bloom-Forming Microalgae in High-Species Phytoplankton Assemblages Under Light-Fluctuating and Low Phosphate Conditions

Bloom-Forming Microalgae in High-Species Phytoplankton Assemblages Under Light-Fluctuating and Low Phosphate Conditions

Membrane Glycerolipid Remodeling Triggered by Nitrogen and Phosphorus Starvation inPhaeodactylum tricornutum

Interspecific variability in phosphorus‐induced lipid remodelling among marine eukaryotic phytoplankton

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