Differences in cell death and cell cycle following fucoidan treatment in high-density HT-29 colon cancer cells

Kim, In‐Hye; Kwon, Mijin; Nam, Taek‐Jeong

doi:10.3892/mmr.2017.6520

Cited by 33 publications

(21 citation statements)

References 30 publications

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“…This condition activates p53 independent pathway involving ribosomal proteins and MDM2 leading to p21-mediated cell cycle arrest and apoptosis. In previous study, fucoidan were induced G1 phase arrest and apoptosis in p53 mutated colon cancer cell line, HT-29 (23). In present study, we examined whether HT-29 cells affect the IGF-IR singnaling pathway involved in cell proliferation during fucoidan.…”

Section: Discussionmentioning

confidence: 83%

“…Numerous studies have investigated the effects of seaweeds on cell death pathways including apoptosis. The inhibition of apoptosis in colon cancer cells promotes tumor growth and tumor progression and imparts tolerance to cytotoxic anticancer drugs (7,22,23,27,(40)(41)(42)(43).…”

Section: Discussionmentioning

confidence: 99%

“…pathway is implicated in receptor-mediated mitogenesis and transformation (35,36). In previous studies, fucoidan inhibited HT-29 cell proliferation by inducing apoptosis (23). In anti-colon cancer-related studies, the epidermal growth factor (EGF) pathway has been investigated, but no studies have investigated the IGF-I pathway (37).…”

Section: Fucoidan Downregulates Insulin-like Growth Factor-i Receptormentioning

confidence: 99%

“…Fucoidan has been shown to exert various biological activities, including antioxidant, anti-inflammatory, anti-angiogenic, anti-coagulant, anti-bacterial, and anticancer effects (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18). Studies have shown anticancer activities of fucoidan in lung, breast, liver, colon, prostate, and bladder cancer cells (19)(20)(21)(22)(23). Fucoidan has also been shown to induce tumor cell injury leading to growth arrest and tumor suppression via apoptosis and cell cycle arrest in various cancer cell types (24)(25)(26)(27)(28)(29).…”

Section: Introductionmentioning

confidence: 99%

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Fucoidan downregulates insulin-like growth factor-I receptor levels in HT-29 human colon cancer cells

Kim

Nam

2018

Oncol Rep

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Fucoidan, a sulfated polysaccharide present in brown seaweed, has demonstrated anticancer activity in lung, breast, liver and colon cells. The insulin-like growth factor (IGF) signaling pathway regulates growth in HT-29 cells through the insulin receptor substrate-1 (IRS-1)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) and Ras/Raf/extracellular signal-regulated kinase (ERK) pathways. The aim of the present study was to investigate whether fucoidan downregulates the IGF-IR signaling pathway in HT-29 human colon cancer cells. Fucoidan treatment (0-1,000 µg/ml) was administered for 24 h in HT-29 cells. First, we investigated IRS-1/PI3K/AKT pathway-related protein expression levels following treatment with fucoidan (0-500 µg/ml) using western blot analysis. Fucoidan significantly inhibited the expression of IGF-IR, PTEN, PI3K and AKT as well as their phosphorylated forms (p-IRS-1, p-PI3K and p-AKT). Next, we investigated the effects of fucoidan on Ras/Raf/ERK pathway‑related protein expression levels in HT-29 cells. Fucoidan significantly inhibited the expression of IGF-IR, Shc, Ras, SOS, Raf and MEK. HT-29 cells were then incubated in the presence of fucoidan (0 or 250 µg/ml), and IGF-I (10 nM) was added for 0 to 60 min. Immunoprecipitation (IP) experiments showed that fucoidan inhibited IGF-I-induced phosphorylation of IGF-IR, PI3K, Shc (IP, IGF-IR), and phosphorylated IRS-1 and PI3K (IP, IRS-1) compared to the control group. Western blot analysis showed that fucoidan inhibited the expression of IGF-I-induced p-IGF-IR/IGF-IR and p-AKT/AKT, but not p-ERK/ERK. In conclusion, the inhibition of cell viability by fucoidan in HT-29 cells may be due to the downregulation of IGF-IR signaling through the main IRS-1/PI3K/AKT pathway. Fucoidan also partially impacted Ras/Raf signaling in the Ras/Raf/ERK pathway. Therefore, we suggest that fucoidan may be a suitable candidate chemopreventive agent in HT-29 colon cancer cells.

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Fucoidan Downregulates Insulin-like Growth Factor-i Receptormentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Fucoidan downregulates insulin-like growth factor-I receptor levels in HT-29 human colon cancer cells

Kim

Nam

2018

Oncol Rep

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“…Ganoderma lucidem polysaccharides inhibits the growth of sarcoma 180 tumors in BALB/c mice . Fucoidan treatment was shown to induce apoptosis through cell cycle arrest in HT‐29 colon cancer cells . Thus, it appears that natural polysaccharides might be a source of potential anticancer therapeutic agents via blockade of the cancer cell cycle.…”

Section: Introductionmentioning

confidence: 99%

Antitumor bioactivity of porphyran extracted from Pyropia yezoensis Chonsoo2 on human cancer cell lines

Yan

et al. 2019

J Sci Food Agric

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BACKGROUND Pyropia yezoensis, rich in porphyran, is a medicine‐edible red alga. In the present study, the physicochemical characteristics, conformational states and antitumor activities of a novel porphyran extracted from the high‐yield algal strain Pyropia yezoensis Chonsoo2 and its two degraded derivatives by gamma irradiation were investigated. RESULTS Pyropia yezoensis porphyran is a water‐soluble, triple‐helical sulfated hetero‐galactopyranose, named PYP. PYP was degraded by gamma irradiation at 20 kGy and 50 kGy, giving two low molecular weight derivatives comprising PYP‐20 and PYP‐50, respectively. PYP with a higher molecular weight has a solution conformation different from PYP‐20 and PYP‐50. Three porphyrans had no toxicity in normal human liver cells (HL‐7702) and showed antitumor effects on Hep3B, HeLa and MDA‐MB‐231. They had better antitumor against HeLa cells, exhibiting a similar inhibition ratio compared to 5‐fluorouracil, with PYP especially exhibiting a higher inhibition ratio than 5‐fluorouracil. With respect to HeLa cells, the different antitumor activities might be related to porphyran molecular weight and solution conformation. Furthermore, the HeLa cell cycle was blocked in the G2/M phase after PYP treatment, leading to cell proliferation inhibition. The induction of cell cycle arrest was related to the changes in the expression of p21, p53, Cyclin B1 and cyclin‐dependent kinase 1. CONCLUSION Pyropia yezoensis porphyran, as applied to medicine and functional food, could potentially be used as a non‐toxic natural adjuvant in cancer therapy. © 2019 Society of Chemical Industry

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Seaweeds: Potential Candidates in Human Colon Cancer Therapy

Pandey¹,

Singh

Shrivastava³

2021

Colon Cancer Diagnosis and Therapy

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Differences in cell death and cell cycle following fucoidan treatment in high-density HT-29 colon cancer cells

Cited by 33 publications

References 30 publications

Fucoidan downregulates insulin-like growth factor-I receptor levels in HT-29 human colon cancer cells

Fucoidan downregulates insulin-like growth factor-I receptor levels in HT-29 human colon cancer cells

Antitumor bioactivity of porphyran extracted from Pyropia yezoensis Chonsoo2 on human cancer cell lines

Seaweeds: Potential Candidates in Human Colon Cancer Therapy

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