Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity

Abstract

SUMMARYPhospholipase and proteinase production and the ability of adhesion to buccal epithelial cells (BEC) of 112 Candida isolates originated from oral cavity of HIV infected patients and from blood and catheter of intensive care unit patients were investigated. The proteinase production was detected by inoculation into bovine serum albumin (BSA) agar and the phospholipase activity was performed using egg yolk emulsion. A yeast suspension of each test strain was incubated with buccal epithelial cells and the number of adherence yeast to epithelial cells was counted. A percentage of 88.1% and 55.9% of Candida albicans and 69.8% and 37.7% of non-albicans Candida isolates produced proteinase and phospholipase, respectively. Non-albicans Candida isolated from catheter were more proteolytic than C. albicans isolates. Blood isolates were more proteolytic than catheter and oral cavity isolates while oral cavity isolates produced more phospholipase than those from blood and catheter. C. albicans isolates from oral cavity and from catheter were more adherent to BEC than non-albicans Candida isolates, but the adhesion was not different among the three sources analyzed. The results indicated differences in the production of phospholipase and proteinase and in the ability of adhesion to BEC among Candida spp. isolates from different sources. This study suggests that the pathogenicity of Candida can be correlated with the infected site.

Increased expression of virulence attributes in oral Candida albicans isolates from human immunodeficiency virus-positive individuals

“…HIV-negative (21) 21 (100) previous studies (Ollert et al, 1995;Koga-Ito et al, 2006;Menezes et al, 2006;Costa et al, 2010;Sacristán et al, 2011). Similar to the results of Ollert et al (1995) and Wu et al (1996), we found increased expression of proteinase activity in isolates from HIV-infected individuals compared with HIV-uninfected subjects.…”

Phospholipase, esterase and hemolytic activities of Candida spp. isolated from onychomycosis and oral lichen planus lesions

“…Secretion of enzymes by microorganism can play an important role in their pathogenesis [6,22]. Candidiasis is an opportunist fungal infection that is caused by several different species of Candida.…”

Oral colonization by yeasts in HIV-positive patients in Brazil

“…However, MANE et al 21 verified production of phospholipase and proteinase in only 59% and 89.7%, Among the isolates of non-albicans species identified in this study, 39% showed phospholipase activity and 21% exhibited proteinase activity. Previous studies also reported that proteinases and phospholipases are produced at high rates in C. albicans, whilst non-albicans Candida species usually present low rates of these enzymes 5,8 . No isolates of C. glabrata, C. lusitaniae or C. guilliermondii exhibited enzymatic activity.…”