Differences in Frequency Distribution of HLA-A2 Subtypes Between North American and Italian White Melanoma Patients: Relevance for Epitope Specific Vaccination Protocols

Player, Mary Anne; Barracchini, Kathleen C.; Simonis, Toni B.; Rivoltini, Licia; Arienti, Flavio; Castelli, Chiara; Mazzocchi, Arabella; Belli, Filiberto; Parmiani, Giorgio; Marincola, Francesco M.

doi:10.1097/00002371-199609000-00005

Cited by 40 publications

(15 citation statements)

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“…Determinations of the HLA haplotypes of cell lines were conducted at the National Institutes of Health HLA Laboratory by analyzing DNA from cell lines through the use of primers that were designed to amplify specific HLA alleles (13). The HLA haplotypes of the cell lines used in this study are indicated in Table I.…”

Section: Hla Typingmentioning

confidence: 99%

Multiple HLA Class II-Restricted Melanocyte Differentiation Antigens Are Recognized by Tumor-Infiltrating Lymphocytes from a Patient with Melanoma

Robbins

El‐Gamil

et al. 2002

The Journal of Immunology

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Dramatic clinical responses were observed in patient 888 following the adoptive transfer of autologous tumor-infiltrating lymphocytes (TIL). Previously, extensive analysis of the specificity of class I-restricted T cells from patient 888 TIL has revealed that these T cells recognize a mutated, as well as several nonmutated tumor Ags. Additional studies that were conducted on TIL from patient 888 indicated that they contained CD4-positive T cells that recognized the autologous tumor that had been induced to express HLA class II molecules. Tumor-reactive CD4-positive T cell clones were isolated from TIL and tested for their ability to react with Ags that are recognized by HLA class I-restricted, melanoma-reactive T cells. Using this approach, T cell clones were identified that recognized an epitope expressed in both the tyrosinase-related protein 1 and tyrosinase-related protein 2 Ags in the context of the HLA-DRβ1*1502 class II gene product. Additional clones were found to recognize an epitope of gp100 in the context of the same HLA-DR restriction element. These observations provide an impetus to develop strategies directed toward generating HLA class II-restricted tumor-reactive T cells.

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Section: Hla Typingmentioning

confidence: 99%

Multiple HLA Class II-Restricted Melanocyte Differentiation Antigens Are Recognized by Tumor-Infiltrating Lymphocytes from a Patient with Melanoma

Robbins

El‐Gamil

et al. 2002

The Journal of Immunology

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“…The HLA class I phenotype of patients was determined on PBMC using sequence-specific primer PCR (12). PCR was also used for molecular subtyping of HLA-A*02 (13).…”

Section: Patients' Selectionmentioning

confidence: 99%

Status of Activation of Circulating Vaccine-Elicited CD8+ T Cells

Nielsen

Monsurró

Migueles

et al. 2000

The Journal of Immunology

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Selective blunting of the status of activation of circulating tumor-specific T cells was invoked to explain their paradoxical coexistence with unhampered tumor growth. By analogy, lack of tumor regression in the face of observable melanoma vaccine-induced T cell responses might be attributed to their status of activation. We enumerated with HLA-A*0201/peptide tetramers (tHLA) vaccine-elicited T cell precursor frequency directly in PBMC of patients with melanoma undergoing vaccination with the HLA-A*0201-associated gp100:209–217(210 M) epitope (g209-2 M). Furthermore, we tested by intracellular (IC)-FACS analysis and quantitative real-time PCR (qRT-PCR) the ability of postvaccination PBMC to produce cytokine in response to challenge with vaccine-related epitopes or vaccine-matched (HLA-A*0201) melanoma cells. Vaccine-induced enhancement of T cell precursor frequency could be detected with tHLA in PBMC from six of eight patients studied at frequencies ranging between 0.3 and 2.3% of the total CD8+ population. Stimulation with vaccine-related epitopes induced IFN-γ expression detectable by IC-FACS or qRT-PCR, respectively, in five and six of these patients. Furthermore, down-regulation of tHLA staining was noted upon cognate stimulation that could be utilized as an additional marker of T cell responsiveness. Finally, we observed in six patients an enhancement of reactivity against vaccine-matched tumor targets that was partly independent of documented vaccine-specific immune responses. A strong correlation was noted between tHLA staining of postvaccination PBMC and IFN-γ expression by the same samples upon vaccine-relevant stimulation and assessed either by IC-FACS or qRT-PCR. Thus, blunting of the status of T cell activation on itself cannot easily explain the lack of clinical responses observed with vaccination.

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“…While some studies on the relationship between miRNAs and gastric cancer have recently been done (reviewed by Saito et al [17]), little work[18]-[21] has been carried out in China. It is important to note that differential gene expression may occur among different ethnic groups[22],[23] as well as within ethnic groups of different spatial localization[24] and within different individuals of the same race[25]. From a Chinese health perspective, studies into the relationship between miRNAs and Chinese sporadic gastric cancer could provide valuable information.…”

Section: Introductionmentioning

confidence: 99%

Elevated expression of mature miR-21 and miR-155 in cancerous gastric tissues from Chinese patients with gastric cancer

Liu

Chen

Lai

et al. 2010

Journal of Biomedical Research

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ObjectiveMicroRNA (miRNA) expression is deregulated in many types of human cancers. We sought to investigate the expression patterns of the miRNAs, miR-21, miR-145 and miR-155 in sporadic gastric cancer in a Chinese population.MethodsTotal RNA was extracted from archived gastric cancer tissues and adjacent non-cancerous tissues from 20 pairs of paraffin-embedded specimens. Expression levels of miR-21, miR-145 and miR-155 were detected by quantitative reverse transcriptase PCR using a specific stem-loop primer, with U6 as the internal reference gene.ResultsThe expression of miR-21 and miR-155 in gastric cancer samples was significantly higher than in paired non-cancerous samples (P < 0.05). There was no statistically significant difference in expression levels of miR-145 between cancerous and non-cancerous tissues (P > 0.05).ConclusionIn Chinese sporadic gastric cancer tissues, the expressions of the oncogenic miR-21 and miR-155 were significantly up-regulated, while the expression of the tumor suppressor miR-145 was decreased, although this decrease was not statistically significant. Thus there is specificity in the miRNA expression pattern in gastric cancers in the Chinese population.

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Differences in Frequency Distribution of HLA-A2 Subtypes Between North American and Italian White Melanoma Patients: Relevance for Epitope Specific Vaccination Protocols

Cited by 40 publications

References 0 publications

Multiple HLA Class II-Restricted Melanocyte Differentiation Antigens Are Recognized by Tumor-Infiltrating Lymphocytes from a Patient with Melanoma

Multiple HLA Class II-Restricted Melanocyte Differentiation Antigens Are Recognized by Tumor-Infiltrating Lymphocytes from a Patient with Melanoma

Status of Activation of Circulating Vaccine-Elicited CD8+ T Cells

Elevated expression of mature miR-21 and miR-155 in cancerous gastric tissues from Chinese patients with gastric cancer

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