Differences in honey bee bacterial diversity and composition in agricultural and pristine environments – a field study

Muñoz-Colmenero, Marta; Baroja-Careaga, Igor; Kovačić, Marin; Filipi, Janja; Puškadija, Zlatko; Kezić, Nikola; Estonba, Andone; Büchler, Ralph; Zarraonaindia, Iratxe

doi:10.1007/s13592-020-00779-w

Cited by 30 publications

(32 citation statements)

References 56 publications

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“…Alteration of gut microbiota influences host metabolism and may affect the storage of energy in honeybees. Some studies had suggested that changes in diversity of gut microbiota are associated with some diseases and opportunistic bacteria 43 , included fungal pathogens and neogregarines 21 . This would benefit honeybees to reduce parasite and pathogenic infection for successful overwintering 24 .…”

Section: Discussionmentioning

confidence: 99%

Overwintering honeybees maintained dynamic and stable intestinal bacteria

Liu

Zhu

Liang

et al. 2021

Sci Rep

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Honeybee is an important pollinator for maintaining ecological balance. However, scientist found the bizarre mass death of bees in winter. Meanwhile, some reported that the differences composed of intestinal bacteria between healthy honeybees and CCD honeybees. It is essential that explored dynamic changes to the intestinal bacteria in overwintering honeybees. We collected bee samples before overwintering, during prophase of overwintering, metaphase of overwintering, anaphase of overwintering, telophase of overwintering, and after overwintering. By using high-throughput sequencing targeting the V3−V4 regions of the 16S rDNA, the abundance of the intestinal bacteria were analyzed in overwintering honeybees. A total of 1,373,886 high-quality sequences were acquired and Proteobacteria (85.69%), Firmicutes (10.40%), Actinobacteria (3.66%), and Cyanobacteria (1.87%) were identified as major components of the intestinal bacteria. All core honeybee intestinal bacteria genera, such as Gilliamella, Bartonella, Snodgrassella, Lactobacillus, Frischella, Commensalibacter, and Bifidobacterium were detected. The abundance of Actinobacteria, Bartonella, and Bifidobacterium increased initially and then decreased in winter honeybees. There were no significant differences in the richness and evenness of the microbiota in overwintering honeybees; however, there was a statistically significant difference in the beta diversity of the intestinal bacteria after overwintering compared with that in other groups. Our results suggested that honeybees maintained their intestinal ecosystem balance, and increased the abundance of gut probiotics in response to environmental and nutrition pressures in winter.

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Section: Discussionmentioning

confidence: 99%

Overwintering honeybees maintained dynamic and stable intestinal bacteria

Liu

Zhu

Liang

et al. 2021

Sci Rep

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“…Jones et al [ 59 ] showed that the broad landscape influenced the diversity of some members of honeybee gastrointestinal microbiota, especially those belonging to Proteobacteria and Firmicutes. Muñoz-Colmenero et al [ 102 ] demonstrated that the environment plays the main role in determining honeybee microbiota, and that agricultural treatments cause disruption to the bacterial community.…”

Section: Honeybee Microbiotamentioning

confidence: 99%

Characterization of Apis mellifera Gastrointestinal Microbiota and Lactic Acid Bacteria for Honeybee Protection—A Review

Nowak

Szczuka

Górczyńska

et al. 2021

Cells

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Numerous honeybee (Apis mellifera) products, such as honey, propolis, and bee venom, are used in traditional medicine to prevent illness and promote healing. Therefore, this insect has a huge impact on humans’ way of life and the environment. While the population of A. mellifera is large, there is concern that widespread commercialization of beekeeping, combined with environmental pollution and the action of bee pathogens, has caused significant problems for the health of honeybee populations. One of the strategies to preserve the welfare of honeybees is to better understand and protect their natural microbiota. This paper provides a unique overview of the latest research on the features and functioning of A. mellifera. Honeybee microbiome analysis focuses on both the function and numerous factors affecting it. In addition, we present the characteristics of lactic acid bacteria (LAB) as an important part of the gut community and their special beneficial activities for honeybee health. The idea of probiotics for honeybees as a promising tool to improve their health is widely discussed. Knowledge of the natural gut microbiota provides an opportunity to create a broad strategy for honeybee vitality, including the development of modern probiotic preparations to use instead of conventional antibiotics, environmentally friendly biocides, and biological control agents.

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“…The factors that contribute to microbiome diversity are highly variable. Bee microbiome composition can be influenced by the evolutionary history 38 , 39 and ecology 40 – 42 of the host species, as well as intraspecific variation stemming from differences in caste 43 – 45 , development stage 35 , 46 , diet 47 – 49 , and infection status 50 . Research with additional bee species is likely to yield further insights into inter- and intra-specific variation in the microbiome.…”

Section: Introductionmentioning

confidence: 99%

Composition and acquisition of the microbiome in solitary, ground-nesting alkali bees

Kapheim

Johnson

Jolley

2021

Sci Rep

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Increasing evidence suggests the microbiome plays an important role in bee ecology and health. However, the relationship between bees and their bacterial symbionts has only been explored in a handful of species. We characterized the microbiome across the life cycle of solitary, ground-nesting alkali bees (Nomia melanderi). We find that feeding status is a major determinant of microbiome composition. The microbiome of feeding larvae was similar to that of pollen provisions, but the microbiome of post-feeding larvae (pre-pupae) was similar to that of the brood cell walls and newly-emerged females. Feeding larvae and pollen provisions had the lowest beta diversity, suggesting the composition of larval diet is highly uniform. Comparisons between lab-reared, newly-emerged, and nesting adult females suggest that the hindgut bacterial community is largely shaped by the external environment. However, we also identified taxa that are likely acquired in the nest or which increase or decrease in relative abundance with age. Although Lactobacillus micheneri was highly prevalent in pollen provisions, it was only detected in one lab-reared female, suggesting it is primarily acquired from environmental sources. These results provide the foundation for future research on metagenomic function and development of probiotics for these native pollinators.

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Differences in honey bee bacterial diversity and composition in agricultural and pristine environments – a field study

Cited by 30 publications

References 56 publications

Overwintering honeybees maintained dynamic and stable intestinal bacteria

Overwintering honeybees maintained dynamic and stable intestinal bacteria

Characterization of Apis mellifera Gastrointestinal Microbiota and Lactic Acid Bacteria for Honeybee Protection—A Review

Composition and acquisition of the microbiome in solitary, ground-nesting alkali bees

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