Differences in Protease Expression of Mutants Bacillus licheniformis F11

Abstract

Marine gastropod, Peronia peronei, from north of the Persian Gulf, protein extraction was investigated using ultrafiltration, reversed-phase high-performance liquid chromatography and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/TOF). The mass spectra showed that, the isolated peptide is most probably Dolabellanin B2 that has been extracted before from other marine mollusk, Dolabella auricularia. The antibacterial activity of purified peptide was assessed against seven bacterial strains (Staphylococcus aureus, Bacillus subtilis, Serratia marcescens, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Proteus mirabilis). Broth micro-dilution method, standard disk diffusion assay and bioautography were used for this aim. According to the results the identified peptide showed strong antibacterial activity against all mentioned bacterial strains. Computational studies through peptide modeling servers followed by 50 ns molecular dynamics simulation and surface electrostatic calculations indicates that this sequence could be most probably related to alpha helical cationic antimicrobial peptides (alpha-CAMPs) with overall charge of +1 at pH 7.

The Potency of Bovine Bone Gelatin as Antihypertensive Agent: A Review

“…The ACE inhibitors sourced from protein are promising for safety. These confirmed ACE inhibitory peptides are containing 10-12 amino acids and having C-terminal hydrophobic side (Baehaki et al, 2016).…”

Combinatorial approach for screening and assessment of multiple therapeutic enzymes from marine isolatePseudomonas aeruginosaAR01

“…Structural stability and activity of Clostridium collagenase were enhanced in the presence of calcium ions. [63][64][65] However, collagenase activity was signicantly inhibited by Fe 2+ .…”

Microbial collagenases: challenges and prospects in production and potential applications in food and nutrition

“…Collagenases have been usually recognized as enzymes that specically attack 'native' and water-soluble denatured collagens. 21,27 The term 'true collagenase' was coined on the basis of their capacity to hydrolyze/digest native bovine achilles tendon collagen. 21 The collagenases are mainly found in animals, microorganisms and plants (Fig.…”

“…Collagenases possess broad substrate specicities to degrade both waterinsoluble 'native' collagens and water-soluble denatured collagens. 27 Due to this unique activity, microbial collagenases play important roles in embryo development, morphogenesis, tissue remodeling, wound healing, and human diseases, such as arthritis, cancer, and atherosclerosis. 6,28 Therefore, microbial 'true' collagenases have gained much attention for their numerous industrial, biotechnological, pharmacological, medicinal and food applications.…”