Differences in the degree of infiltration of local anesthesia according to the site of injection in rats

Tateno, Ken; Inoue, Kazuo; Sato, Toshio; Fukayama, Haruhisa

doi:10.1016/j.tripleo.2008.03.034

Cited by 9 publications

(11 citation statements)

References 4 publications

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“…Infiltration anesthetic by injection into the mucobuccal fold allows injectate to pass through the alveolar bone (Tateno et al, 2008). Here we used tooth pulp stimulation-evoked dEMG recordings to demonstrate that the infiltration injection of lidocaine in adult rats results in rapid (< 5 min) reduction of the JOR, thus validating the technique as an effective method for anesthesia of pulpal afferents.…”

Section: Shammentioning

confidence: 76%

Pain Fiber Anesthetic Reduces Brainstem Fos after Tooth Extraction

Badral¹,

Davies²,

Kim³

et al. 2013

J Dent Res

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We recently demonstrated that pain-sensing neurons in the trigeminal system can be selectively anesthetized by co-application of QX-314 with the TRPV1 receptor agonist, capsaicin (QX cocktail). Here we examined whether this new anesthetic strategy can block the neuronal changes in the brainstem following molar tooth extraction in the rat. Adult male Sprague-Dawley rats received infiltration injection of anesthetic 10 min prior to lower molar tooth extraction. Neuronal activation was determined by immunohistochemistry for the proto-oncogene protein c-Fos in transverse sections of the trigeminal subnucleus caudalis (Sp5C). After tooth extraction, c-Fos-like immunoreactivity (Fos-LI) detected in the dorsomedial region of bilateral Sp5C was highest at 2 hrs (p < .01 vs. naïve ipsilateral) and declined to pre-injury levels by 8 hrs. Pre-administration of the QX cocktail significantly reduced to sham levels Fos-LI examined 2 hrs after tooth extraction; reduced Fos-LI was also observed with the conventional local anesthetic lidocaine. Pulpal anesthesia by infiltration injection was confirmed by inhibition of the jaw-opening reflex in response to electrical tooth pulp stimulation. Our results suggest that the QX cocktail anesthetic is effective in reducing neuronal activation following tooth extraction. Thus, a selective pain fiber 'nociceptive anesthetic' strategy may provide an effective local anesthetic option for dental patients in the clinic.

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Section: Shammentioning

confidence: 76%

Pain Fiber Anesthetic Reduces Brainstem Fos after Tooth Extraction

Badral¹,

Davies²,

Kim³

et al. 2013

J Dent Res

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“…4 However, eventually, all residual local anesthetic is absorbed gradually from the capillary vessels into the systemic circulation and is metabolized by hepatic cytochrome P450. 5,6 In this study, infiltration anesthesia was applied to the mandibular angle (A) at the thin cortical bone and low bone density areas, and the mandibular body (B) at the thick cortical bone and high bone density areas. In the mandibular angle (A), the cortical bone width and bone density were 0.6 mm and 140 mg/cm 2 , respectively.…”

Section: Discussion Infiltration Mechanisms Of Local Anesthetic To Jamentioning

confidence: 99%

Lidocaine Concentration in Mandibular Bone After Subperiosteal Infiltration Anesthesia Decreases With Elevation of Periosteal Flap and Irrigation With Saline

Ogawa

Watanabe

Kawaai

et al. 2014

Anesthesia Progress

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It has been reported that the action of infiltration anesthesia on the jawbone is attenuated significantly by elevation of the periosteal flap with saline irrigation in clinical studies; however, the reason is unclear. Therefore, the lidocaine concentration in mandibular bone after subperiosteal infiltration anesthesia was measured under several surgical conditions. The subjects were 48 rabbits. Infiltration anesthesia by 0.5 mL of 2% lidocaine with 1 : 80,000 epinephrine (adrenaline) was injected into the right mandibular angle and left mandibular body, respectively. Under several surgical conditions (presence or absence of periosteal flap, and presence or absence of saline irrigation), both mandibular bone samples were removed at a fixed time after subperiosteal infiltration anesthesia. The lidocaine concentration in each mandibular bone sample was measured by high-performance liquid chromatography. As a result, elevation of the periosteal flap with saline irrigation significantly decreased the lidocaine concentration in the mandibular bone. It is suggested that the anesthetic in the bone was washed out by saline irrigation. Therefore, supplemental conduction and/or general anesthesia should be utilized for long operations that include elevation of the periosteal flap with saline irrigation.

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“…Fourteen male Wistar rats (10 weeks at age, body weight 273-308 g) were used for this experiment. After the induction of ether general anesthesia, the rat's limbs were fixed with pins and the heart was exposed for perfusion fixation in the dorsal position, in accordance with the method of Tateno et al [6] with minimal modifications.…”

Section: Methodsmentioning

confidence: 99%

“…Recently, the use of an automatic injector with a slow and constant speed has been recommended for reducing injection pain [5]. In our previous study of the infiltration area of an anesthetic in the maxilla of rats, we reported that an injection into the buccal side represented wider diffusion of local anesthetics than that into the lingual side [6].…”

Section: Introductionmentioning

confidence: 99%

Diffusion patterns of anesthetic solution in the infiltration anesthesia depending on injection sites in the mandible of rats

Yamada

Sato

Fukayama

2011

Oral Science International

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a b s t r a c tPurpose: This study was conducted for the morphological and quantitative evaluation of the diffusible patterns of local anesthetic depending on the site of injection in infiltration anesthesia. Experiments: The mandibular first molars of rats were anesthetized by the local infiltration of 30 l of 2% lidocaine containing trypan blue at a distance of 2 mm from the gingival margin on either the lingual or buccal side over the distal root apex of the first molar. Frozen sections were prepared to measure the volume of the infiltrated anesthetic using volume-calculating software. Results: On the lingual side, the local anesthetic infiltrated into the mouth floor and spread backward in a sagittal direction at an infiltration volume of 8.36 ± 7.34 mm 3 . On the buccal side, the anesthetic solution was restricted to the injected area and the infiltration volume was 2.22 ± 1.62 mm 3 .Conclusion The present results indicate that the infiltration volume of local anesthetics is very different between the lingual and buccal sides.

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Differences in the degree of infiltration of local anesthesia according to the site of injection in rats

Cited by 9 publications

References 4 publications

Pain Fiber Anesthetic Reduces Brainstem Fos after Tooth Extraction

Pain Fiber Anesthetic Reduces Brainstem Fos after Tooth Extraction

Lidocaine Concentration in Mandibular Bone After Subperiosteal Infiltration Anesthesia Decreases With Elevation of Periosteal Flap and Irrigation With Saline

Diffusion patterns of anesthetic solution in the infiltration anesthesia depending on injection sites in the mandible of rats

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