Differences in the Electrophoretic Characteristics of Bovine Rete Testis Fluid and Plasma From the Cauda Epididymidis

Amann, R. P.; Killian, Gary J.; Benton, Adia

doi:10.1530/jrf.0.0350321

Cited by 44 publications

(11 citation statements)

References 18 publications

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“…The seminiferous tubular fluid (d) contained a number of protein bands which were absent from blood serum. This confirms previous results that the testis secretes specific proteins into the lumen (Amann et al 1973;Koskimies & Kormano 1973;Turner et al 1979). The observation that many of the testicular protein bands were reduced in concentration or absent from the fluid of the caput epididymidis suggests that some of the testicular proteins were reabsorbed by efferent ducts or the initial segment.…”

Section: Biochemical Analysis Of Secretionsupporting

confidence: 92%

Secretion of macromolecules by the rat epididymis

1982

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Sperm maturation in the rat epididymis is dependent on the secretion of specific proteins by the epididymal epithelium and subsequent interaction of these proteins with spermatozoa. Evidence has shown that fertility and motility development of epididymal spermatozoa may be impaired by interfering the interaction of these proteins with spermatozoa. When the spermatozoa reach the cauda epididymidis, they are fully mature but their longevity is maintained by being stored in a quiescent state in the cauda. The unique ionic medium therein (low Na+, low Ca2+, high K+ and low pH) suppresses sperm motility and hence reserving energy for the vital processes of capacitation and fertilization. During ejaculation, when the spermatozoa are mixed with the copious secretion from the accessory glands they burst into vigorous motility. This results from an influx of sodium coupled to efflux of K+ and H+ across the mature sperm membrane. In the presence of a peptide secreted by the cauda epididymidis, these ionic events activate the already mature but otherwise inactive spermatozoa to full motility.

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Section: Biochemical Analysis Of Secretionsupporting

confidence: 92%

Secretion of macromolecules by the rat epididymis

1982

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“…Over the years, several investigators have observed that many of the proteins present in epididymal luminal fluid, in a variety of species, differ from those present in serum and rete testis fluid (Alumot et al, 1971;Amann et al, 1973;Jones, 1987;Olson and Hinton, 1985;Turner et al, 1979). Moreover, the combination of various techniques of micropuncture with gel electrophoresis made it possible to determine that the luminal composition of epididymis-specific proteins varies along the length of the epididymis (Olson and Hinton, 1985;Turner et al, 1979;Wong et al, 1981).…”

Section: Macromolecular Luminal Fluid Microenvironmentmentioning

confidence: 99%

Epididymal epithelium: Its contribution to the formation of a luminal fluid microenvironment

Hinton

Palladino

1995

Microscopy Res & Technique

209

109

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To understand the process of sperm maturation, an understanding of interactions between the spermatozoa with the luminal fluid microenvironment and with the epididymal epithelium is necessary. The composition of epididymal luminal fluid of several species is well documented but the manner by which the epididymis contributes to the formation of this specialized milieu is not so well understood. A major role played by the epididymis is to finely regulate the movement of molecules into and out of the lumen. This ensures that as spermatozoa progress along the duct they are exposed to a continually changing, but optimal environment necessary for their maturation and survival. This review focusses on our current understanding of the contributions of the epididymal epithelium to the formation of a specialized luminal fluid microenvironment. The role of the blood-epididymis barrier, the composition of the epididymal luminal fluid, the permeability properties of the epididymal epithelium, and recent studies on a number of luminal fluid proteins and expression of the genes which encode these proteins are discussed.

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“…The fate and significance of most of these proteins is unknown. Recently, it has been shown electrophoretically that bovine epididymal plasma from the cauda region contains at least three proteins not detected in the rete testis fluid or serum (Amann, Killian & Benton, 1973).…”

Section: Reabsorbedmentioning

confidence: 99%