Differences in the phospholipid profile of melanocytes and melanoma cells irradiated with UVA and treated with cannabigerol and cannabidiol

Łuczaj, Wojciech; Dobrzyńska, Izabela; Skrzydlewska, Elżbieta

doi:10.1038/s41598-023-43363-9

Cited by 4 publications

(1 citation statement)

References 61 publications

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“…The non-psychomimetic phytocannabinoid, cannabigerol (CBG), was evaluated for its potential anticancer effect in human cancer cell lines, including melanoma and glioblastoma [12,13]. Although the pathways activated by CBG were not yet clarified, recently, CBG was demonstrated to bind the kinase active site of EGFR and inhibit its intracellular kinase activity, inducing apoptosis in the EGFR-overexpressing A549 and A431 cell lines [14].…”

Section: Introductionmentioning

confidence: 99%

Cannabigerol Induces Autophagic Cell Death by Inhibiting EGFR-RAS Pathways in Human Pancreatic Ductal Adenocarcinoma Cell Lines

Zeppa,

Aguzzi,

Morelli

et al. 2024

IJMS

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Pancreatic ductal adenocarcinoma (PDAC) is the most frequent infiltrating type of pancreatic cancer. The poor prognosis associated with this cancer is due to the absence of specific biomarkers, aggressiveness, and treatment resistance. PDAC is a deadly malignancy bearing distinct genetic alterations, the most common being those that result in cancer-causing versions of the KRAS gene. Cannabigerol (CBG) is a non-psychomimetic cannabinoid with anti-inflammatory properties. Regarding the anticancer effect of CBG, up to now, there is only limited evidence in human cancers. To fill this gap, we investigated the effects of CBG on the PDAC cell lines, PANC-1 and MIAPaCa-2. The effect of CBG activity on cell viability, cell death, and EGFR-RAS-associated signaling was investigated. Moreover, the potential synergistic effect of CBG in combination with gemcitabine (GEM) and paclitaxel (PTX) was investigated. MTT was applied to investigate the effect of CBG on PDAC cell line viabilities. Annexin-V and Acridine orange staining, followed by cytofluorimetric analysis and Western blotting, were used to evaluate CBG’s effect on cell death. The modulation of EGFR-RAS-associated pathways was determined by Western blot analysis and a Milliplex multiplex assay. Moreover, by employing the MTT data and SynergyFinder Plus software analysis, the effect of the combination of CBG and chemotherapeutic drugs was determined.

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Section: Introductionmentioning

confidence: 99%

Cannabigerol Induces Autophagic Cell Death by Inhibiting EGFR-RAS Pathways in Human Pancreatic Ductal Adenocarcinoma Cell Lines

Zeppa,

Aguzzi,

Morelli

et al. 2024

IJMS

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Skin cells protection against UVA radiation – The comparison of various antioxidants and viability tests

Gęgotek,

Mucha,

Skrzydlewska

2024

Biomedicine & Pharmacotherapy

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Full‐spectrum cannabidiol reduces UVB damage through the inhibition of TGF‐β1 and the NLRP3 inflammasome

Urrutia‐Ortega,

Valencia,

Ispanixtlahuatl‐Meraz

et al. 2024

Photochem & Photobiology

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The thermodynamic characteristics, antioxidant potential, and photoprotective benefits of full‐spectrum cannabidiol (FS‐CBD) against UVB‐induced cellular death were examined in this study. In silico analysis of CBD showed antioxidant capacity via proton donation and UV absorption at 209.09, 254.73, and 276.95 nm, according to the HAT and SPLET methodologies. FS‐CBD protected against UVB‐induced bacterial death for 30 min. FS‐CBD protected against UVB‐induced cell death by 42% (1.5 μg/mL) and 35% (3.5 μg/mL) in an in vitro keratinocyte cell model. An in vivo acute irradiated CD‐1et/et mouse model (UVB‐irradiated for 5 min) presented very low photoprotection when FS‐CBD was applied cutaneously, as determined by histological analyses. In vivo skin samples showed that FS‐CBD regulated inflammatory responses by inhibiting the inflammatory markers TGF‐β1 and NLRP3. The docking analysis showed that the CBD molecule had a high affinity for TGF‐β1 and NLRP3, indicating that protection against inflammation might be mediated by blocking these proinflammatory molecules. This result was corroborated by the docking interactions between CBD and TGF‐β1 and NLRP3, which resulted in a high affinity and inhibition of both proteins The present work suggested a FS‐CBD moderate photoprotective agent against UVB light‐induced skin damage and that this effect is partially mediated by its anti‐inflammatory activity.

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Differences in the phospholipid profile of melanocytes and melanoma cells irradiated with UVA and treated with cannabigerol and cannabidiol

Cited by 4 publications

References 61 publications

Cannabigerol Induces Autophagic Cell Death by Inhibiting EGFR-RAS Pathways in Human Pancreatic Ductal Adenocarcinoma Cell Lines

Cannabigerol Induces Autophagic Cell Death by Inhibiting EGFR-RAS Pathways in Human Pancreatic Ductal Adenocarcinoma Cell Lines

Skin cells protection against UVA radiation – The comparison of various antioxidants and viability tests

Full‐spectrum cannabidiol reduces UVB damage through the inhibition of TGF‐β1 and the NLRP3 inflammasome

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