Differences in ultrastructural organization of amyloid as revealed by sensitivity or resistance to induced proteolysis

Romhányi, G

doi:10.1007/bf00548215

Cited by 55 publications

(9 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunocytochemistry showed positive staining with antibodies against substance P but no reactivity for anti-amyloid AA, confirming that these were primary amyloid deposits. 4 There was no evidence of amyloid within the acellular polypoid mass that was attached to the right coronary cusp. This had the staining characteristics of post-mature fibrin' and samples subjected to transmission electron microscopy showed degenerate fibrillar material and cellular debris.…”

mentioning

confidence: 90%

Amyloid, thrombosis, and acute myocardial infarction in association with a bicuspid aortic valve.

Groves

Douglas-Jones²,

Hall³

1993

Heart

View full text Add to dashboard Cite

The clinical diagnosis was inferior myocardial infarction, which was presumed to have resulted from a right coronary embolus arising from the abnormal aortic right coronary cusp. The patient was anticoagulated with warfarin, remained afebrile, and made an uncomplicated recovery. The electrocardiogram showed normalisation of the inferior T waves without the development of Q waves. Blood cultures were negative.Six weeks later the aortic valve was replaced by a tilting disc prosthesis. Examination of the native aortic valve confirmed that it was originally bicuspid with congenital fusion of the right and non-coronary cusps. The right coronary portion of the fused cusp was totally replaced by a protuberant mass that impinged on the left coronary cusp and spread inferiorly towards the adjacent left ventricular wall. There was no macroscopic evidence of infection. The coronary orifices were normally situated and the cooling pattern of the myocardium during the administration of cold cardioplegia was uniform, implying the absence of obstructive coronary artery disease. Specimens from the excised valve were sterile on culture.Postoperative recovery was uneventful and the patient has since remained fit and well. An exercise stress test performed two months after aortic valve replacement was normal.

show abstract

mentioning

confidence: 90%

Amyloid, thrombosis, and acute myocardial infarction in association with a bicuspid aortic valve.

Groves

Douglas-Jones²,

Hall³

1993

Heart

View full text Add to dashboard Cite

show abstract

“…3 of them had a monoclonal (M) component in the serum or in the urine, whereas case 3 had clinically primary amyloidosis associated with hypogammaglobulinemia. Liver amy loid deposits, as well as those of the other organs, proved to be resistant to trypsin di gestion according to the method of Romhanyi [12], this feature being characteristic of the AL type. Liver deposits of cases 1 and 3 showed a lesser affinity for Congo Red and a yellower birefringence under the polarizing light microscope than those of the other cases.…”

Section: Discussionmentioning

confidence: 99%

Different Morphologic Aspects and Clinical Features in Massive Hepatic Amyloidosis

et al. 1984

View full text Add to dashboard Cite

4 cases of massive hepatic amyloidosis are reported with special reference to their clinical profiles and histologic features. On the basis of these data, two different clinical and histologic courses of the disease can be distinguished. 2 patients showed marked hepatomegaly without cholestasis, whereas in the other 2 the clinical picture was characterized by much less pronounced hepatomegaly, but by severe and progressive intrahepatic cholestasis. The time course of the disease seems to be different in the two forms, the cholestatic form being more rapidly fatal than the other.

show abstract

“…The fi rst hint that amyloid is made up of chemically different amyloid "structures" came from the oxidation and digestion of amyloid in tissue sections as performed by Romhányi (1971Romhányi ( , 1972. He demonstrated susceptible and resistant amyloid deposits, the former belonging to patients with long-standing infl ammations and the latter belonging to other forms of amyloid lacking chronic infl ammations.…”

Section: Before the Chemical Identifi Cationmentioning

confidence: 97%

Congo Red Staining of Amyloid: Improvements and Practical Guide for a More Precise Diagnosis of Amyloid and the Different Amyloidoses

Linke

Protein Reviews

View full text Add to dashboard Cite

Congo red (CR) is the most popular dye used as a probe for diagnosing amyloidosis, a very heterogeneous group of diseases with more than 23 chemically different amyloid syndromes of men and animals, leading to more than 400 different individual diseases. Congo red binding increases the natural anisotropy of amyloid, indicating that the elongated and planar CR molecules are aligned parallel to the axis of the amyloid α fi brila and to each other, thereby revealing a structure of amyloid. This structure was established to represent fi brils of similar dimensions, although the amyloid fi brils can be composed of many unrelated proteins. This CR-induced (positive) anisotropy displaying a green color is the hallmark of all amyloids, and is therefore used in the diagnosis of amyloidosis. The specifi city of this criterion, however, is based on very stringent conditions of staining and evaluation. This review will focus on the understanding of the CR staining procedure, its mechanism and, in particular, on its recent practical improvements by increasing the sensitivity of the CR procedure, so that minute and the earliest amyloid deposits in the course of amyloidosis can now be reliably detected in patients. This enables a very early diagnosis in the course of the disease before irreversible organ damage might have occurred, and widens the options for a successful therapy. In addition, the central role of the CR diagnostic procedure and evasion of common pitfalls in arriving at a pathogenetically exact classifi cation must only be based on the chemical nature of the amyloid deposits and not on the soluble precursors of the many different amyloidoses will be highlighted. The proposed bench-tobedside algorithm will enable the physician to arrive at the exact diagnosis for therapeutic considerations. Finally, some possible future applications of CR and analogues will be presented.

show abstract

Differences in ultrastructural organization of amyloid as revealed by sensitivity or resistance to induced proteolysis

Cited by 55 publications

References 31 publications

Amyloid, thrombosis, and acute myocardial infarction in association with a bicuspid aortic valve.

Amyloid, thrombosis, and acute myocardial infarction in association with a bicuspid aortic valve.

Different Morphologic Aspects and Clinical Features in Massive Hepatic Amyloidosis

Congo Red Staining of Amyloid: Improvements and Practical Guide for a More Precise Diagnosis of Amyloid and the Different Amyloidoses

Contact Info

Product

Resources

About