Differences in Vanadocene Dichloride and Cisplatin Effect on MOLT-4 Leukemia and Human Peripheral Blood Mononuclear Cells

Abstract

Modern chemotherapy is interested in developing new agents with high efficiency of treatment in low-dose medication strategies, lower side toxicity and stronger specificity to the tumor cells. Vanadocene dichloride (VDC) belongs to the group of the most promising metallocene antitumor agents; however, its mechanism of action and cytotoxicity profile are not fully understood. In this paper we assess cytotoxic effects of VDC in comparison to cisplatin using opposite prototype of cells; human peripheral blood mononuclear (PBMCs) cells and human acute lymphoblastic leukemia cell line (MOLT-4). Our findings showed cytotoxic effect of VDC on leukemia cells, but unfortunately on human peripheral blood mononuclear cells as well. VDC induces apoptosis in leukemia cells; the induction is, however, lower than that of cisplatin, and in contrary to cisplatin, VDC does not induce p53 up-regulation. Cytotoxic effect of VDC on leukemia cells is less pronounced than that of cisplatin and more pronounced in PBMCs than in MOLT-4 cells.

Amaryllidaceae Alkaloids of Different Structural Types from Narcissus L. cv. Professor Einstein and Their Cytotoxic Activity

“…All experiments were performed at least three times with triplicate measurements at each drug concentration per experiment. The viability was quantified as described in Havelek et al according to the following formula: (%) viability = (Asample − Ablank)/(Acontrol − Ablank) × 100, where A is the absorbance of the employed WST-1 formazan measured at 440 nm [43]. The viability of the treated cells was normalized to the viability of cells treated with 0.1% DMSO (Sigma-Aldrich, St. Louis, MO, USA) as a vehicle control.…”

Inorganic Coordination Chemistry: Where We Stand in Cancer Treatment?

“…This complex showed a strong activity in vitro against several tumor cells. In addition, in vivo studies demonstrated antitumor properties with vanadocene dichloride [114]. Some vanadocene derivatives present cytotoxic effect against T-lymphocytic leukemia cells, where the mechanism used evolves the DNA damage and p53 activation [115].…”

Chelidonine and Homochelidonine Induce Cell Death through Cell Cycle Checkpoints and MAP Kinase Pathways

“…Tris base (10 mM) was then added to the wells to solubilize the bound SRB dye, and the plates were then read at 565 nm using a multiwell microplate reader, specifically Tecan Infinite M200 (Tecan Group Ltd., Männedorf, Switzerland). The percentage of growth inhibition was calculated using the following formula: (%) growth = (A565sample -A565blank) / (A565control -A565blank) x 100, where A565 is the absorbance of solubilized SRB dye measured at 565 nm [26].…”