Differences in virulence within the species Bacteroides gingivalis

Steenbergen, T. J. M. van; Delemarre, F. G. A.; Namavar, F.; Graaff, J. de

doi:10.1007/bf00393930

Cited by 98 publications

(92 citation statements)

References 11 publications

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“…Results of a study performed with an infection model using rodents suggested that P. gingivalis can be divided into organisms with invasive traits and organisms with noninvasive traits depending on the capsular structure of the organism (7,39). However, little information regarding the predominant types of P. gingivalis involved in chronic periodontitis is available.…”

Section: Discussionmentioning

confidence: 99%

“…It has been postulated that in the oral cavity the innate host defense system limits the spread of oral bacteria by maintaining an intact epithelial barrier (13). Porphyromonas gingivalis, a gramnegative black-pigmented anaerobe, has been found to adhere to various oral surfaces in periodontitis patients (9,39) and has also been detected within gingival tissues in vivo (31). It has also been shown that this organism invades oral epithelial cells in vitro (22,32,33).…”

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confidence: 99%

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Functional Differences among FimA Variants of Porphyromonas gingivalis and Their Effects on Adhesion to and Invasion of Human Epithelial Cells

Nakagawa¹,

et al. 2002

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Fimbriae of Porphyromonas gingivalis, a periodontopathogen, play an important role in its adhesion to and invasion of host cells. The fimA genes encoding fimbrillin (FimA), a subunit protein of fimbriae, have been classified into five types, types I to V, based on nucleotide sequences. We previously reported that P. gingivalis with type II fimA was strongly associated with adult periodontitis. In the present study, we compared the abilities of recombinant FimA (rFimA) types I to V to adhere to and invade human gingival fibroblasts (HGF) and a human epithelial cell line (HEp-2 cells) by using rFimA-conjugated microspheres (rFimA-MS). There were no significant differences in the abilities of the rFimA-MS to adhere to HGF; however, the adhesion of type II rFimA-MS to HEp-2 cells was significantly greater than those of other types of rFimA-MS. We also observed that type II rFimA-MS invaded epithelial cells and accumulated around the nuclei. These adhesion and invasion characteristics were eliminated by the addition of antibodies to type II rFimA and ␣5␤1-integrin. In contrast, Arg-Gly-Asp-Ser peptide and a synthetic peptide of proline-rich protein C had negligible inhibitory effects. Furthermore, P. gingivalis strain HW24D1 with type II fimA adhered to cells and invaded them more than strains with other fimA genotypes. These results suggest that type II FimA can bind to epithelial cells most efficiently through specific host receptors.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Functional Differences among FimA Variants of Porphyromonas gingivalis and Their Effects on Adhesion to and Invasion of Human Epithelial Cells

Nakagawa¹,

et al. 2002

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“…High genetic heterogeneity has been reported for P. gingivalis (22), and strains have been shown to differ in their capacity to cause soft tissue destruction (23,24) and bone resorption in animal models (25). Along with host-related susceptibility factors, differences in virulence between strains of the same species might help explain why disease progresses more rapidly in some individuals than in others, and why a species can be detected in both symptomatic and asymptomatic cases (26).…”

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Prevalence and Clonal Analysis of Porphyromonas gingivalis in Primary Endodontic Infections

Siqueira¹,

Rôças²,

Silva³

2008

Journal of Endodontics

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“…Theoretically, this could be indicative of a heterogeneous species with subpopulations of low and high pathogenicity. Indeed, numerous studies have revealed variations among strains with regard to pathogenicity in experimental infections (8,9,12,17,21) and in the expression of potential virulence factors (16,36,38,39,40). In addition, specific genotypes of the fimA gene encoding fimbrillin and specific heteroduplex types of the ribosomal intergenic spacer region have been linked to periodontal disease in humans (3,10).…”

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confidence: 99%

Evidence of Recombination in Porphyromonas gingivalis and Random Distribution of Putative Virulence Markers

Frandsen

Poulsen

Curtis³

et al. 2001

Infect Immun

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The association of Porphyromonas gingivalis to periodontal disease is not clearly understood. Similar proportions of P. gingivalis may be cultivated from both inactive and actively degrading periodontal pockets. Differences in virulence among strains of P. gingivalis exist, but the molecular reason for this remains unknown. We examined the population structure of P. gingivalis to obtain a framework in which to study pathogenicity in relation to evolution. Phylogenetic trees derived from the sequencing of fragments of four housekeeping genes, ahp, thy, rmlB, and infB, in 57 strains were completely different with no correlation between clustering of strains in the four dendrograms. Combining the various alleles of the four gene fragments sequenced resulted in 41 different sequence types. The index of association, I A , based on a single representative of each sequence type was 0.143 ؎ 0.202, indicating a population at linkage equilibrium. Inclusion of all isolates for the calculation of I A resulted in a value of 0.206 ؎ 0.171. This suggests an epidemic population structure supported by the finding of genetically identical strains in different parts of the world. We observed a random distribution of two virulence-associated mobile genetic elements, the ragB locus and the insertion sequence IS1598, among 132 strains tested. In conclusion, P. gingivalis has a nonclonal population structure characterized by frequent recombination. Our study suggests that particular genotypes, possibly with increased pathogenic potential, may spread successfully in the human population.

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Differences in virulence within the species Bacteroides gingivalis

Cited by 98 publications

References 11 publications

Functional Differences among FimA Variants of Porphyromonas gingivalis and Their Effects on Adhesion to and Invasion of Human Epithelial Cells

Functional Differences among FimA Variants of Porphyromonas gingivalis and Their Effects on Adhesion to and Invasion of Human Epithelial Cells

Prevalence and Clonal Analysis of Porphyromonas gingivalis in Primary Endodontic Infections

Evidence of Recombination in Porphyromonas gingivalis and Random Distribution of Putative Virulence Markers

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