Differences in Virus-Replicating Capacity Among Plant Species Inoculated with Tobacco Mosaic Virus

Cheo, P. C.

doi:10.1094/phyto-61-1010

Cited by 30 publications

(9 citation statements)

References 3 publications

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“…In essence, Pto functions as a patch to return the inherent strong basal immune defense response in light of functional AvrPtoB. Our model also satisfies explanations for R-gene evolution, innate mechanisms of defense that make plants immune to most potential pathogens (19,46,74) and observed stochastic and kinetic defense response differences between susceptible and resistant plants including "spreading" local lesion phenotypes that are part of an R-gene mediated HR (i.e. a leaky patch).…”

Section: Discussionmentioning

confidence: 54%

Quantitative Proteomic Analysis of Bean Plants Infected by a Virulent and Avirulent Obligate Rust Fungus

Lee

Feng

Campbell

et al. 2009

Molecular & Cellular Proteomics

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Plants appear to have two types of active defenses, a broad-spectrum basal system and a system controlled by R-genes providing stronger resistance to some pathogens that break the basal defense. However, it is unknown if the systems are separate entities. Therefore, we analyzed proteins from leaves of the dry bean crop plant Phaseolus vulgaris using a high-throughput liquid chromatography tandem mass spectrometry method. By statistically comparing the amounts of proteins detected in a single plant variety that is susceptible or resistant to infection, depending on the strains of a rust fungus introduced, we defined basal and R-gene-mediated plant defenses at the proteomic level. The data reveal that some basal defense proteins are potential regulators of a strong defense weakened by the fungus and that the R-gene modulates proteins similar to those in the basal system. The results satisfy a new model whereby R-genes are part of the basal system and repair disabled defenses to reinstate strong resistance. Molecular & Cellular Proteomics 8:19 -31, 2009.

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Section: Discussionmentioning

confidence: 54%

Quantitative Proteomic Analysis of Bean Plants Infected by a Virulent and Avirulent Obligate Rust Fungus

Lee

Feng

Campbell

et al. 2009

Molecular & Cellular Proteomics

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“…We chose pea and tobacco protoplasts, as pea is a host for PSbMV but not for TMV or TEV [20,21], whereas tobacco is a host for TMV and TEV bu: not for PSbMV [22]. As can be seen from Fig.…”

Section: Discussionmentioning

confidence: 99%

The 5′ untranslated region from pea seedborne mosaic potyvirus RNA as a translational enhancer in pea and tobacco protoplasts

et al. 1992

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We have exploited the transient expression of foreign genes introduced into plant protoplasts to investigate the effect of the pea seedborne mosaic potyvirus (PSbMV) 5′ untranslated region (5′UTR) on the level of gene expression in pea and tobacco protoplasts. The plant viral 5′UTRs were found to increase translation significantly in comparison to a plasmid containing no 5'UTR of viral origin. The enhancement effect or the 5′UTRs of PSbMV and tobacco etch potyvirus (TEV) was found to be similar in pea and tobacco protoplasts, indicating a host‐independent role of the potyviral 5′UTRs in enhancing gene expression. Translational enhancement of the two potyviral 5′UTRs was similar to that or the 5′UTR of tobacco mosaic virus (TMV). This observation makes it attractive to use potyviral 5′UTRs as general translational enhancers in future genetic transformations of plants.

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“…Saintpaulia ionantha was previously reported to be probably immune to TMV infection (Cheo and Gerard, 1971). Based on the results of ELISA and bioassay analysis of S. ionantha extracts after mechanical inoculation, it seems that S. ionantha sustains an asymptomatic, low-level subliminal infection after mechanical inoculation with TMV.…”

Section: Resultsmentioning

confidence: 96%

“…Moreover, data presented in Table 3 demonstrate that infection occurs even when viral RNA is used as inoculum, effectively eliminating the possibility of residual antigens or infectivity. Cheo and Gerard (1971) originally classified S. ionantha as probably immune to TMV infection based on their inability to recover any virus after mechanical inoculation. One difference between our study and their study is that we used a more-concentrated inoculum.…”

Section: Resultsmentioning

confidence: 99%

“…Holmes (1946) reported that S. ionantha demonstrated no increase of virus titer and no manifestation of disease after mechanical inoculation with tobacco mosaic virus (TMV) and concluded that this species is insusceptible to infection. Later, Cheo and Gerard (1971) reported that S. ionantha 'Wild White' was probably immune to TMV infection, since there was no detectable recovery of virus under standard conditions and inoculated tissue remained asymptomatic.…”

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confidence: 99%

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Tobacco Mosaic Virus Subliminal Infection of African Violet

Sulzinski¹,

Jurkonie²,

Adonizio³

1994

jashs

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`Wild White' African violet (Saintpaulia ionantha H. Wendl.) was previously reported to be probably immune to tobacco mosaic virus (TMV) infection. In this study, 15 other S. ionantha cultivars were mechanically inoculated with 200 μg TMV/ml sodium phosphate buffer. Two weeks postinoculation, tissue was harvested and assayed for TMV infection by a) TMV-specific enzyme-linked immunosorbent assay and b) bioassay on the local lesion host, `Samsun NN' tobacco (Nicotiana tabacum L.). There was evidence of TMV infection in directly inoculated tissue of each of the 15 S. ionantha cultivars but not in noninoculated tissue or in mock-inoculated control plants. The small amount of virus recovered from inoculated tissue was shown to be the result of de facto viral infection and not the detection of residual inoculum. Postinoculation treatment with ultraviolet light significantly enhanced virus recovery in directly inoculated tissue. These results suggest that S. ionantha is not immune to TMV infection and that this host undergoes an asymptomatic subliminal infection by TMV.

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Differences in Virus-Replicating Capacity Among Plant Species Inoculated with Tobacco Mosaic Virus

Cited by 30 publications

References 3 publications

Quantitative Proteomic Analysis of Bean Plants Infected by a Virulent and Avirulent Obligate Rust Fungus

Quantitative Proteomic Analysis of Bean Plants Infected by a Virulent and Avirulent Obligate Rust Fungus

The 5′ untranslated region from pea seedborne mosaic potyvirus RNA as a translational enhancer in pea and tobacco protoplasts

Tobacco Mosaic Virus Subliminal Infection of African Violet

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