Differences of endogenous polyamines and putative genes associated with paraquat resistance in goosegrass (<i>Eleusine indica L.</i>)

Luo, Qiyu; Wei, Jiping; Dong, Zhaoxia; Shen, Xuefeng; Chen, Yong

doi:10.1101/618074

Cited by 5 publications

(5 citation statements)

References 33 publications

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“…7 In E. indica, there is evidence that polyamine transporters play a key role in paraquat resistance, since paraquat has a similar structure to endogenous polyamines that are carried in vacuoles. 54 In our case, we can affirm that the paraquat resistance of the R-15 population was due to the reduced translocation of the herbicide, although we do not know if the herbicide was sequestered in the vacuoles.…”

Section: ■ Discussionmentioning

confidence: 54%

Multiple Herbicide Resistance Evolution: The Case of Eleusine indica in Brazil

Vázquez-García

Cruz

Rojano-Delgado

et al. 2021

J. Agric. Food Chem.

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The occurrence of multiple herbicide resistant weeds has increased considerably in glyphosate-resistant soybean fields in Brazil; however, the mechanisms governing this resistance have not been studied. In its study, the target-site and nontarget-site mechanisms were characterized in an Eleusine indica population (R-15) with multiple resistance to the acetyl-CoA carboxylase (ACCase) inhibitors, glyphosate, imazamox, and paraquat. Absorption and translocation rates of 14C-diclofop-methyl14C-imazamox and 14C-glyphosate of the R-15 population were similar to those of a susceptible (S-15) population; however, the R-15 population translocated ∼38% less 14C-paraquat to the rest of plant and roots than the S-15 population. Furthermore, the R-15 plants metabolized (by P450 cytochrome) 55% and 88% more diclofop-methyl (conjugate) and imazamox (imazamox–OH and conjugate), respectively, than the S-15 plants. In addition, the Pro-106-Ser mutation was found in the EPSPS gene of this population. This report describes the first characterization of the resistance mechanisms in a multiple herbicide resistant weed from Brazil.

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Section: ■ Discussionmentioning

confidence: 54%

Multiple Herbicide Resistance Evolution: The Case of Eleusine indica in Brazil

Vázquez-García

Cruz

Rojano-Delgado

et al. 2021

J. Agric. Food Chem.

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“…Natural polymorphic variations in a gene (LHR1) for a plasma membrane-localized polyamine transporter in Arabidopsis caused variations in uptake of paraquat that correlated with sensitivity to paraquat (89). Endogenous polyamines have been shown to play a role in paraquat-resistant E. indica (90). Evolved paraquat resistance in weeds has not yet been attributed to selection for orthologs of PAR1 or LHR1.…”

Section: Reduced Translocation and Vacuolar Sequestrationmentioning

confidence: 99%

Mechanisms of evolved herbicide resistance

Gaines

Duke

Morran

et al. 2020

Journal of Biological Chemistry

432

493

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The widely successful use of synthetic herbicides over the past 70 years has imposed strong and widespread selection pressure, leading to the evolution of herbicide resistance in hundreds of weed species. Both target-site resistance (TSR) and non-target-site resistance (NTSR) mechanisms have evolved to most herbicide classes. TSR often involves mutations in genes encoding the protein targets of herbicides, affecting the binding of the herbicide either at or near catalytic domains or in regions affecting access to them. Most of these mutations are non-synonymous single-nucleotide polymorphisms, but polymorphisms in more than one codon or entire codon deletions have also evolved. Some herbicides bind multiple proteins, making TSR mechanisms more difficult to evolve. Increased amounts of protein target, by increased gene expression or by gene duplication, is an important, albeit less common, TSR mechanism. NTSR mechanisms include reduced absorption or translocation and increased sequestration or metabolic degradation. The mechanisms that can contribute to NTSR are complex and often involve genes that are members of large gene families. For example, enzymes involved in herbicide metabolism–based resistances include cytochromes P450, glutathione-S-transferases, glucosyl and other transferases, aryl acylamidase, and others. Both TSR and NTSR mechanisms can combine at the individual level to produce higher resistance levels. The vast array of herbicide-resistance mechanisms for generalist (NTSR) and specialist (TSR and some NTSR) adaptations that have evolved over a few decades illustrate the evolutionary resilience of weed populations to extreme selection pressures. These evolutionary processes drive herbicide and herbicide-resistant crop development and resistance management strategies.

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“…[8][9][10] Polyamines have been identified to be associated with the resistance and detoxification mechanism in the paraquatresistant biotypes of goosegrass. [11][12][13] The structures of polyamines are similar to that of paraquat (Fig. 1).…”

Section: Introductionmentioning

confidence: 63%

“…ha −1 paraquat (Syngenta Corporation, Shanghai, China) in our previous studies. 11,13 After treatment of paraquat at 30, 60, and 90 min, separately collected R and S goosegrass were named R1, R2, R3, S1, S2, and S3. And then, they were frozen in liquid nitrogen and stored in tin foil at −80 °C.…”

Section: Goosegrass Planting and Paraquat Treatmentsmentioning

confidence: 99%

“…A significantly increased transcript level of the EiKCS gene, which was previously named the PqE gene, was observed in a paraquatresistant goosegrass under paraquat stress, as compared to the expression in susceptible goosegrass. 13 Phylogenetic analysis showed that EiKCS was closest to the KCS gene (EC 2.3.1.199) in rice (O_sativa_subsp_indica_A2X281) (Fig. 2(A)).…”

Section: Construction Of the Kcsox Rice And Confirmation Of Overexpressionmentioning

confidence: 99%

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Overexpression of EiKCS confers paraquat‐resistance in rice (Oryza sativaL.) by promoting the polyamine pathway

Luo

Chen

Zhu

et al. 2021

Pest Management Science

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BACKGROUND: Paraquat is used widely as one of the bipyridine herbicides, which generates reactive oxygen species to cause cell death. With a growing number of paraquat-resistant weeds, the mechanism of paraquat-resistance in plants remains unclear. This research verified the functions of a previously confirmed putative paraquat-resistant gene, EiKCS, from paraquat-resistant goosegrass by genetic engineering in a single overexpressing line in rice.RESULTS: Overexpression of EiKCS improved paraquat resistance in transgenic rice (KCSox). Pre-applied (12 h) exogenous spermidine (1.5 mmol L −1 ), alleviated the injury of paraquat in rice. Paraquat induced injury in KCSox was 19.57%, which was lower than 32.22% injury it induced in wild-type (WT) rice. The paraquat-resistant mechanism was through the increased activity of antioxidant enzymes and the overproduction of endogenous polyamines. The spermine content in KCSox was more than 30 ∼g mL −1 , while that in WT rice was less than 5 ∼g mL −1 . Quantitative proteomics showed that ⊎-ketoacyl-coenzyme A (CoA) synthase ( 51.81 folds) encoded by the transgenic EiKCS gene promoted the synthesis of the proteins involved with the polyamine pathway. The synthesized putrescine was promoted by the arginine decarboxylase (ADC) pathway. The spermidine synthase I (1.10-fold) and three eceriferum cofactors (CERs) were responsive to the paraquat stress. We validated putrescine (C 18 H 20 N 2 O 2 ) spermidine (C 28 H 31 N 3 O 3 ), and spermine (C 38 H 42 N 4 O 4 ) in this study.CONCLUSION: EiKCS encoding ⊎-ketoacyl-CoA synthase from goosegrass has been shown as an ideal candidate gene for engineering genetically modified organism (GMO) crops, as its overexpression does not only bring paraquat-resistance, but also have potential benefits without decreasing yield and rice grain quality.

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Differences of endogenous polyamines and putative genes associated with paraquat resistance in goosegrass (Eleusine indica L.)

Cited by 5 publications

References 33 publications

Multiple Herbicide Resistance Evolution: The Case of Eleusine indica in Brazil

Multiple Herbicide Resistance Evolution: The Case of Eleusine indica in Brazil

Mechanisms of evolved herbicide resistance

Overexpression of EiKCS confers paraquat‐resistance in rice (Oryza sativaL.) by promoting the polyamine pathway

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