Differences of immune responses between Tongcheng (Chinese local breed) and Large White pigs after artificial infection with highly pathogenic porcine reproductive and respiratory syndrome virus

Liang, Wan; Li, Zhenhong; Wang, Peng; Fan, Pengcheng; Zhang, Qingde; Wang, Yan; Xu, Xuewen; Liu, Bang

doi:10.1016/j.virusres.2016.02.004

Cited by 31 publications

(45 citation statements)

References 46 publications

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“…Several studies have shown that alternative splicing could affect host immune response mainly through apoptosis-related biological processes [46], DNA damage response [47,48], and RIG-I pathway [49,50]. ese results were consistent with our previous results that TC pigs and LW pigs had difference immune response to PRRSV infection [16].…”

Section: Discussionsupporting

confidence: 92%

“…e PRRSV challenge experiments were conducted by our previous study [16]. Briefly, a total of twenty-four five-weekold pigs including twelve TC pigs and twelve LW pigs were selected to perform the artificial challenge experiment as described previously.…”

Section: Animals Experimental Design and Sample Collectionmentioning

confidence: 99%

“…Transcriptome analysis of lung dendritic cells (DCs) derived from Pietrain and Duroc revealed Duroc reacted more distinctly and strongly than Pietrain during various periods of PRRSV infection [15]. e transcriptome study of PAMs in Tongcheng (TC) pigs and Large White (LW) pigs showed that two breeds had different immune responses to PRRSV infection [16]. It is mainly manifested that the differentially expressed genes in TC pigs are enriched in activation of leukocyte extravasation and suppression of apoptosis while LW pigs are enriched in the suppression of Gαq and PI3K-AKT signaling [17].…”

Section: Introductionmentioning

confidence: 99%

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Global Analysis of Alternative Splicing Difference in Peripheral Immune Organs between Tongcheng Pigs and Large White Pigs Artificially Infected with PRRSV In Vivo

Zhang

Xue

Hang

et al. 2020

BioMed Research International

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Alternative splicing (AS) plays a significant role in regulating gene expression at the transcriptional level in eukaryotes. Flexibility and diversity of transcriptome and proteome can be significantly increased through alternative splicing of genes. In the present study, transcriptome data of peripheral immune organs including spleen and inguinal lymph nodes (ILN) were used to identify AS difference between PRRSV-resistant Tongcheng (TC) pigs and PRRSV-susceptible Large White (LW) pigs artificially infected with porcine reproductive and respiratory syndrome virus (PRRSV) in vivo. The results showed that PRRSV infection induced global alternative splicing events (ASEs) with different modes. Among them, 373 genes and 595 genes in the spleen and ILN of TC pigs, while 458 genes and 560 genes in the spleen and ILN of LW pigs had significantly differential ASEs. Alternative splicing was subject to tissue-specific and lineage-specific regulation in response to PRRSV infection. Enriched GO terms and pathways showed that genes with differential ASEs played important roles in transcriptional regulation, immune response, metabolism, and apoptosis. Furthermore, a splicing factor associated with apoptosis, SRSF4, was significantly upregulated in LW pigs. Functional analysis on apoptosis associated genes was validated by RT-PCR and DNA sequencing. These findings revealed different response to PRRSV between PRRSV-resistant TC pigs and PRRSV-susceptible LW pigs at the level of alternative splicing, suggesting the potential relationship between AS and disease resistance to PRRSV.

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Section: Discussionsupporting

confidence: 92%

Section: Animals Experimental Design and Sample Collectionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Global Analysis of Alternative Splicing Difference in Peripheral Immune Organs between Tongcheng Pigs and Large White Pigs Artificially Infected with PRRSV In Vivo

Zhang

Xue

Hang

et al. 2020

BioMed Research International

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“…In this study, we showed that the gene expression levels of IFN α (2.64-fold), IL8 (2.37-fold), and IL12 p40 (4.89-fold) were significantly higher in DPL than Landrace pigs after stimulating PBMCs with CpG ODN. Other studies showed that the gene expression levels of IFN α, IL8, and IL12 p40 were significantly higher after stimulation with CpG ODN or infection with pathogens in swine (Dar et al, 2010; Borghetti et al, 2012; Liang et al, 2016). Therefore, the significantly higher levels of theses cytokines in DPL pigs may play crucial roles in DPL disease resistance.…”

Section: Discussionmentioning

confidence: 97%

CpG Oligodeoxynucleotides Induce Differential Cytokine and Chemokine Gene Expression Profiles in Dapulian and Landrace Pigs

Yang

Wang

et al. 2016

Front. Microbiol.

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Oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODN) mimic the immunostimulatory activity of microbial DNA by interacting with Toll-like receptor 9 (TLR9) to activate both the innate and adaptive immune responses in different species. However, few studies have been published to compare the effects of CpG ODN on different pig breeds. Therefore, in this study, whole blood gene expression profiles of DPL and Landrace pigs treated with CpG ODN were studied using RNA-seq technology. Five Hundred differentially expressed genes (DEGs) were identified between the two breeds. DPL pigs had significantly higher number of immune-relevant DEGs than the Landrace pigs after CpG ODN treatment. Pathway analysis showed that cytokine-cytokine receptor interaction and chemokine signaling pathway were the major enriched pathways of the immune-relevant DEGs. Further in vitro experiments showed that PBMCs of the DPL pigs had significantly higher levels of TLR9 mRNA than those of the Landrace pigs, both before and after CpG ODN stimulation. Cytokine and chemokine induction in the PBMCs of both breeds were also measured after CpG ODN stimulation. Our data showed that mRNA levels of cytokines (IFNα, IL8, IL12 p40) and chemokines (CXCL9, CXCL13) were significantly higher in the PBMCs of the DPL pigs than those of the Landrace pigs. Taken together, our data provide new information regarding the pig breed difference in response to CpG ODN stimulation and that higher levels of TLR9 mRNA in DPL pigs may be a major contributor for disease resistance.

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“…The artificial infection experiment of PRRSV and its results were introduced in detail in our previous paper (Liang et al, 2016). Briefly, twenty-four five-week-old piglets were randomly selected: 12 from the Large White pig population and 12 from the Tongcheng pig population.…”

Section: Methodsmentioning

confidence: 99%

<i>GBP6</i>: differential expression in pulmonary alveolar macrophages under PRRSV infection and association with blood parameters of its missense mutation

et al. 2017

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The GBP6 gene belongs to the gene family of interferon-gamma inducible GTPases, and is located in a previously characterized QTL region of porcine reproductive and respiratory syndrome virus (PRRSV) resistance in SSC4. In the current study, RT-PCR assay revealed that the GBP6 gene was widely distributed but highly expressed in spleen and lung. Under PRRSV infection, the GBP6 gene was up-regulated and differentially expressed in the pulmonary alveolar macrophages (PAMs) of Large White and Tongcheng pigs. Multiple sequences alignment and phylogenetic analysis revealed that GBP6 is highly conserved across species. We validated and analyzed the polymorphism of a missense mutation (n.932A>G, rs322187731) in the F2 resource population of Duroc × Erhualian, which changed the Valine (V) to Methionine (M) in the 300th residue of porcine GBP6. Through a linear mixed effects model, the polymorphism of the mutation (n.932A>G, rs322187731) was significantly associated with five haematological traits, namely platelet count at 20 days (PLT-20), plateletcrit at 20 days (PCT-20), white blood cell at 33 days (WBC-33), neutrophil count at 33 days (NE-33) and red blood cell count at 80 days (RBC-80). The findings presented here provide basic information for the function and possible involvement of the GBP6 gene in immune regulation and disease resistance. ______________________________________________________________________________________

show abstract

Differences of immune responses between Tongcheng (Chinese local breed) and Large White pigs after artificial infection with highly pathogenic porcine reproductive and respiratory syndrome virus

Cited by 31 publications

References 46 publications

Global Analysis of Alternative Splicing Difference in Peripheral Immune Organs between Tongcheng Pigs and Large White Pigs Artificially Infected with PRRSV In Vivo

Global Analysis of Alternative Splicing Difference in Peripheral Immune Organs between Tongcheng Pigs and Large White Pigs Artificially Infected with PRRSV In Vivo

CpG Oligodeoxynucleotides Induce Differential Cytokine and Chemokine Gene Expression Profiles in Dapulian and Landrace Pigs

<i>GBP6</i>: differential expression in pulmonary alveolar macrophages under PRRSV infection and association with blood parameters of its missense mutation

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