Differences of Spermatozoa Concentration Analysis Between Manual and Automatic Methods

Abstract

The examination of sperm concentration in the laboratory is the calculation of the number and motility using a microscope or using a device. There are still some clinicians who doubt the accuracy of the sperm count results using a semen analyzer rather than using the manual method. This study aims is to determine the differences of the sperm concentration examination between the manual method and the automatic method. Subjects in this study were patients who carried out semen analysis tests at the Clinical Pathology Laboratory of RSIA "Restu Ibu" Sragen from June to August 2020. The object of this research is the examination of sperm concentration, using a manual method using a hemocytometer and an automatic method using the LensHooke ™ SQA X1 Pro. The results of statistical tests using the Mann Whitney methods show that the significance value (p) was 0.960, which means that there was no difference in the results of the sperm concentration examination between the manual method and the automatic method. Result of this research shows that there is no weakness or significant difference if compared between manual and automatic methods.

Sprague Dawley Rat Spermatozoa Quality Due to Bee Pollen Consumption

“…Macroscopic observations were made on sperm color, sperm consistency, and sperm pH (Ismawatie et al 2021). The quality of spermatozoa was observed using a microscope by counting the number of motile spermatozoa in several areas of the field of view using a light microscope at 40x magnification, by estimating the progressively moving spermatozoa from the entire field of view and the estimated area and expressed as a percentage of motile (%).…”

“…Calculations on the concentration of spermatozoa were carried out using a Counting Chamber with a dilution of 1: 500 to formol saline (Ismawatie et al 2021). The morphology of spermatozoa was observed by staining with Eosin Nigrosin viewed under a light microscope at 40x magnification.…”