Différenciation morphologique de deux espèces sympatriques :Phlebotomus perniciosusNewstead 1911 etPhlebotomus longicuspisNitzulescu 1930 (Diptera : Psychodidae)

Abstract

Morphological distinction between two sympatric species: Phlebotomus perniciosus and Phlebotomus longicuspis (Diptera: Psychodidae). In this article we present a new approach for the distinction of two sympatric species of Phlebotomus vectors of visceral leishmaniasis due to Leishmania infantum in Algeria. In countries around the Mediterranean Sea, Phlebotomus perniciosus Newstead 1911 revealed the existence of atypic morphes which cause erroneous identifi cations wihich consequently can lead to confusions with the closely similar species Phlebotomus longicuspis Nitzulescu 1930. We used the morphological criteria of identifi cation as a fi rst approach on morphological variation of these species. Besides the genital characteristics, the numbers of median coxite were included. This approach allows determining the existence of particular morphological types of Phlebotomus perniciosus in the investigated areas. Résumé. Nous rapportons les résultats sur la distinction entre deux espèces sympatriques du genre Phlebotomus, vecteurs de la leishmaniose viscérale à Leishmania infantum en Algérie. Dans les pays circum mediterranéen, Phlebotomus perniciosus Newstead 1911 a révélé l'existence de morphes atypiques qui sont à l'origine d'erreurs d'identifi cation et par conséquent d'une confusion avec l'espèce voisine Phlebotomus longicuspis Nitzulescu 1930. Nous avons utilisé les critères morphologiques comme une première approche sur les variations morphologiques de ces espèces. L'identifi cation repose, outre les caractères des appareils génitaux, sur le dénombrement des soies médianes des coxites qui permettent de déterminer l'existence de morphotypes particuliers de Phlebotomus perniciosus dans les localités prospectées.

Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS

“…However, in eastern Algeria, the atypical morphs of P . perniciosus were until now never reported [ 55 ]. Here, these results were supported by the morphological analyses of aedeagi and Cytb sequencing from P .…”

Spatial relations among environmental factors and phlebotomine sand fly populations (Diptera: Psychodidae) in central and southern Morocco

“…All male P. perniciosus collected showed atypical forms of copulatory valves. This atypical form of P. perniciosus (PNA) has been reported in northern (Pesson et al as well as in southern Morocco (Boussaa et al It was identified also in Tunisia (Ghrab et al Boudabous et al and probably in Algeria (Berchi et al Eight collected species ( S. dreyfussi, P. alexandri, P. bergeroti, S. christophersi, S. africana, P. chabaudi, P. chadlii , and P. kazeruni ) are not abundant (less than 2%) in our study area and we could not determine their distribution. For the abundant species ( S. minuta, S. fallax, P. perniciosus, P. papatasi, P. sergenti, P. longicuspis , and P. ariasi ), the geographical distribution was presented according to some environmental variables (Figure ).…”

“…This atypical form of P. perniciosus (PNA) has been reported in northern (Pesson et al 2004) as well as in southern Morocco . It was identified also in Tunisia (Ghrab et al 2006, Boudabous et al 2012 and probably in Algeria (Berchi et al 2007). Eight collected species (S. dreyfussi, P. alexandri, P. bergeroti, S. christophersi, S. africana, P. chabaudi, P. chadlii, and P. kazeruni) are not abundant (less than 2%) in our study area and we could not determine their distribution.…”

Understanding the factors that determine the emergence of anthroponotic cutaneous leishmaniasis due to Leishmania tropica: Comparison of the density and mitochondrial lineage of Phlebotomus sergenti between endemic and free areas in Morocco

“…The captured sand flies were stored in 70% alcohol. Male and female specimens were separated and morphologically identified using taxonomic keys (Rioux and Golvan, 1969;Rioux et al, 1978;Leger et al, 1983;El Sawaf et al, 1989;Gil Collado et al,1989;Benabdennbi et al, 1999;Berchi et al, 2007;Saezet al, 2018). The specimens were placed in Marc Andre solution and heated to boiling point, and finally mounted on slides under a coverslip using Berlese solution.…”