Different Age-Induced Changes in Rhizosphere Microbial Composition and Function of Panax ginseng in Transplantation Mode

Wang, Qiuxia; Sun, Huifeng; Li, Meijia; Xu, Chenglu; Zhang, Yayu

doi:10.3389/fpls.2020.563240

Cited by 27 publications

(14 citation statements)

References 75 publications

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“…Soil nutrients are considered an important factor in improving the stress resistance and yield of American ginseng [ 39 ]. Additionally, the transplantation mode directly affects the changes of soil nutrients during its growth [ 40 ]. Similarly, in the present study, the VY4 and CRY4 groups (4 th year of American ginseng cultivation soil) were rich in AN, OP, and AK ( Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of American Ginseng Cultivation on Bacterial Community Structure and Responses of Soil Nutrients in Different Ecological Niches

Chang

Jia

et al. 2022

J. Microbiol. Biotechnol.

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American ginseng ( Panax quinquefolium L.) is a perennial herbaceous plant widely cultivated in China, Korea, the United States, and Japan due to its multifunctional properties. In northwest China, transplanting after 2-3 years has become the main mode of artificial cultivation of American ginseng. However, the effects of the cultivation process on the chemical properties of the soil and bacterial community remain poorly understood. Hence, in the present study, high-throughput sequencing and soil chemical analyses were applied to investigate the differences between bacterial communities and nutrition driver factors in the soil during the cultivation of American ginseng. The responses of soil nutrition in different ecological niches were also determined with the results indicating that the cultivation of American ginseng significantly increased the soluble nutrients in the soil. Moreover, the bacterial diversity fluctuated with cultivation years, and 4-year-old ginseng roots had low bacterial diversity and evenness. In the first two years of cultivation, the bacterial community was more sensitive to soil nutrition compared to the last two years. Proteobacteria, Actinobacteria, Gemmatimonadetes, Acidobacteria, Firmicutes, and Bacteroidetes dominated the bacterial community regardless of the cultivation year and ecological niche. With the increase of cultivation years, the assembly of bacterial communities changed from stochastic to deterministic processes. The high abundance of Sphingobium , Novosphingobium , and Rhizorhabdus enriched in 4-years-old ginseng roots was mainly associated with variations in the available potassium (AK), total phosphorus (TP), total potassium (TK), and organic matter (OM).

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Section: Discussionmentioning

confidence: 99%

Effects of American Ginseng Cultivation on Bacterial Community Structure and Responses of Soil Nutrients in Different Ecological Niches

Chang

Jia

et al. 2022

J. Microbiol. Biotechnol.

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“…The results showed that two spatial variables (PCNM 1 and 3), two climatic variables (MAT and MAP), and three edaphic variables (pH, AN, and AP) were significant in driving bacterial community turnover ( Figure 3B ). Besides the geographic location, plant age could influence the assembly of rhizosphere microbiota ( Micallef et al, 2009 ; Marques et al, 2014 ; Huang et al, 2020 ; Wang et al, 2020 ). Plants can select different microbes at the different stages of development, presumably for specific benefits ( Chaparro et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Taxonomic Structure of Rhizosphere Bacterial Communities and Its Association With the Accumulation of Alkaloidal Metabolites in Sophora flavescens

Chen

Chang

et al. 2021

Front. Microbiol.

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Plant secondary metabolites (SMs) play a crucial role in plant defense against pathogens and adaptation to environmental stresses, some of which are produced from medicinal plants and are the material basis of clinical efficacy and vital indicators for quality evaluation of corresponding medicinal materials. The influence of plant microbiota on plant nutrient uptake, production, and stress tolerance has been revealed, but the associations between plant microbiota and the accumulation of SMs in medicinal plants remain largely unknown. Plant SMs can vary among individuals, which could be partly ascribed to the shift in microbial community associated with the plant host. In the present study, we sampled fine roots and rhizosphere soils of Sophora flavescens grown in four well-separated cities/counties in China and determined the taxonomic composition of rhizosphere bacterial communities using Illumina 16S amplicon sequencing. In addition, the association of the rhizosphere bacterial microbiota with the accumulation of alkaloids in the roots of S. flavescens was analyzed. The results showed that S. flavescens hosted distinct bacterial communities in the rhizosphere across geographic locations and plant ages, also indicating that geographic location was a larger source of variation than plant age. Moreover, redundancy analysis revealed that spatial, climatic (mean annual temperature and precipitation), and edaphic factors (pH and available N and P) were the key drivers that shape the rhizosphere bacterial communities. Furthermore, the results of the Mantel test demonstrated that the rhizosphere bacterial microbiota was remarkably correlated with the contents of oxymatrine, sophoridine, and matrine + oxymatrine in roots. Specific taxa belonging to Actinobacteria and Chloroflexi were identified as potential beneficial bacteria associated with the total accumulation of matrine and oxymatrine by a random forest machine learning algorithm. Finally, the structural equation modeling indicated that the Actinobacteria phylum had a direct effect on the total accumulation of matrine and oxymatrine. The present study addresses the association between the rhizosphere bacterial communities and the accumulation of alkaloids in the medicinal plant S. flavescens. Our findings may provide a basis for the quality improvement and sustainable utilization of this medicinal plant thorough rhizosphere microbiota manipulation.

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“…Previous studies have investigated root rot in P. notoginseng using the amplicon technologies, such as 16S/18S rRNA sequencing and ITS, and mostly focused on fungi. Even though other species such as Panax ginseng and Panax quinquefolius have been studied ( Xing et al, 2010 ; Vendan et al, 2012 ; Ji et al, 2018 ; Deng et al, 2020 ; Wang Q. et al, 2020 ), reports on the endophytes and rhizosphere microorganisms of P. notoginseng remain limited and no studies have investigated the microbial community of root rot P. notoginseng using metagenomics.…”

Section: Introductionmentioning

confidence: 99%

Structure and Function of Rhizosphere Soil and Root Endophytic Microbial Communities Associated With Root Rot of Panax notoginseng

et al. 2022

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Panax notoginseng (Burk.) F. H. Chen is a Chinese medicinal plant of the Araliaceae family used for the treatment of cardiovascular and cerebrovascular diseases in Asia. P. notoginseng is vulnerable to root rot disease, which reduces the yield of P. notoginseng. In this study, we analyzed the rhizosphere soil and root endophyte microbial communities of P. notoginseng from different geographical locations using high-throughput sequencing. Our results revealed that the P. notoginseng rhizosphere soil microbial community was more diverse than the root endophyte community. Rhodopseudomonas, Actinoplanes, Burkholderia, and Variovorax paradoxus can help P. notoginseng resist the invasion of root rot disease. Ilyonectria mors-panacis, Pseudomonas fluorescens, and Pseudopyrenochaeta lycopersici are pathogenic bacteria of P. notoginseng. The upregulation of amino acid transport and metabolism in the soil would help to resist pathogens and improve the resistance of P. notoginseng. The ABC transporter and gene modulating resistance genes can improve the disease resistance of P. notoginseng, and the increase in the number of GTs (glycosyltransferases) and GHs (glycoside hydrolases) families may be a molecular manifestation of P. notoginseng root rot. In addition, the complete genomes of two Flavobacteriaceae species and one Bacteroides species were obtained. This study demonstrated the microbial and functional diversity in the rhizosphere and root microbial community of P. notoginseng and provided useful information for a better understanding of the microbial community in P. notoginseng root rot. Our results provide insights into the molecular mechanism underlying P. notoginseng root rot and other plant rhizosphere microbial communities.

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Different Age-Induced Changes in Rhizosphere Microbial Composition and Function of Panax ginseng in Transplantation Mode

Cited by 27 publications

References 75 publications

Effects of American Ginseng Cultivation on Bacterial Community Structure and Responses of Soil Nutrients in Different Ecological Niches

Effects of American Ginseng Cultivation on Bacterial Community Structure and Responses of Soil Nutrients in Different Ecological Niches

Taxonomic Structure of Rhizosphere Bacterial Communities and Its Association With the Accumulation of Alkaloidal Metabolites in Sophora flavescens

Structure and Function of Rhizosphere Soil and Root Endophytic Microbial Communities Associated With Root Rot of Panax notoginseng

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