Different approaches for the detection of thrombin by an electrochemical aptamer-based assay coupled to magnetic beads

Centi, Sonia; Messina, Germán A.; Tombelli, Sara; Palchetti, Ilaria; Mascini, Marco

doi:10.1016/j.bios.2008.01.020

Cited by 98 publications

(48 citation statements)

References 35 publications

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“…In the case of anti-Cp-Biotin-Strept-MBs immunosensors, we observed experimentally that a blocking step with 2 % BSA was necessary in order to minimize nonspecific binding of immunoreagents. This was in agreement with the findings obtained by other authors [30]. …”

Section: Resultssupporting

confidence: 94%

Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin

et al. 2013

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Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBsimmunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphtol generated after 1-naphtyl phosphate addition. Linear ranges of calibration curves and detection limits were 0.1-1000 mg/mL and 0.040 mg/mL (Protein A-MBs), and 0.025-20 mg/mL and 0.018 mg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.

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Section: Resultssupporting

confidence: 94%

Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin

et al. 2013

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“…[1,[4][5][6][7] These reported aptamer-based biosensors have shown potential in many research fields and practical applications, such as in basic biological studies, high throughput drug screening, disease diagnosis, nanotechnology, and materials science. However, most of the reported electrochemical aptamer-based biosensors have employed labeled aptamers with redox-active units, [8][9][10] redox proteins, [11,12] or eletrocatalytic nanoparticles. [13] Most of the developed optical aptamer-based biosensors involved the use of fluorophores [14][15][16] , quantum dots [17,18] , or metallic nanoparticles [19] to tag the aptamers or capture probes.…”

Section: Introductionmentioning

confidence: 99%

A Sensitive, Label‐Free, Aptamer‐Based Biosensor Using a Gold Nanoparticle‐Initiated Chemiluminescence System

2010

Chemistry A European J

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We report a label-free, aptamer-based chemiluminescent biosensor. The biosensor relies upon the catalytic activity of unmodified gold nanoparticles (AuNPs) on the luminol-H(2)O(2) chemiluminescence (CL) reaction, and the interaction of unmodified AuNPs with the aptamer. The unmodified AuNPs can effectively differentiate unstructured and folded aptamer. The binding of the aptamer with the target can induce the AuNP aggregation in the presence of 0.5 M NaCl, and after aggregation the catalytic activity of the AuNPs on the luminol-H(2)O(2) CL reaction is greatly enhanced. During the assay, no covalent functionalization of the AuNPs or aptamer is required. The detection limit of thrombin was estimated to be as low as 26 fM, and the sensitivity was more than 4 orders of magnitude better than that of known AuNP-based colorimetric methods for the detection of thrombin. This aptamer-based biosensor offers the advantages of being simple, cheap, rapid, and sensitive.

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“…The first aptamer was generally immobilized onto gold via a thiol [56][57][58][59][60] and, more recently, magnetic beads [61,62]. The labels of the second aptamer were either redox molecules, nanocomposites [60], nanoparticles [57,63], quantum dots [58,59] or enzymes, with catalytic activity that transformed the substrate into an electroactive product [56,64,65].…”

Section: Sandwich-type G4 Electrochemical Aptasensormentioning

confidence: 99%

Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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Guanine-rich nucleic acids are able to self-assemble into G-quadruplex four-stranded secondary structures, which are found at the level of telomeric regions of chromosomes, oncogene promoter sequences and other biologically-relevant regions of the genome. Due to their extraordinary stiffness and biological role, G-quadruples become relevant in areas ranging from structural biology to medicinal chemistry, supra-molecular chemistry, nanotechnology and biosensor technology. In addition to classical methodologies, such as circular dichroism, nuclear magnetic resonance or crystallography, electrochemical methods have been successfully used for the rapid detection of the conformational changes from single-strand to G-quadruplex. This review presents recent advances on the G-quadruplex electrochemical characterization and on the design and applications of G-quadruplex electrochemical biosensors, with special emphasis on the G-quadruplex aptasensors and hemin/G-quadruplex peroxidase-mimicking DNAzyme biosensors.

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Different approaches for the detection of thrombin by an electrochemical aptamer-based assay coupled to magnetic beads

Cited by 98 publications

References 35 publications

Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin

Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin

A Sensitive, Label‐Free, Aptamer‐Based Biosensor Using a Gold Nanoparticle‐Initiated Chemiluminescence System

Guanine Quadruplex Electrochemical Aptasensors

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