Different approaches to establish infertile rooster

Ghadimi, Fereshteh; Malek, S. R. A.; Zhandi, Mahdi; Zaghari, M.; Piryaei, Abbas; Moslehifar, Parham; Rajabinejad, Alireza

doi:10.1016/j.anireprosci.2017.08.019

Cited by 6 publications

(3 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most previous large‐animal models of transplantation have entailed the use of radiation to deplete endogenous spermatogenesis, 43‐45 as considerable injury to the haematopoietic system is provoked by the toxicity of alkylating agents; however, alkylsulfonate busulfan is favourable to the establishment of testicular recipient models for cellular transplantation in mice 46 and monkeys 47 . We therefore utilized the approach reported by Hermann et al to eliminate endogenous spermatogenesis while simultaneously counteracting the damage to the hematopoietic system.…”

Section: Resultsmentioning

confidence: 99%

Primary culture of germ cells that portray stem cell characteristics and recipient preparation for autologous transplantation in the rhesus monkey

Yuan

Sun

Wang

et al. 2022

J Cellular Molecular Medi

View full text Add to dashboard Cite

Fertility preservation for prepubertal cancer patients prior to oncologic treatment is an emerging issue, and non‐human primates are considered to constitute suitable models due to the limited availability of human testicular tissues. However, the feasibility of spermatogonial stem cell (SSC) propagation in vitro and autologous testicular germ cell transplantation in vivo requires further exploration in monkeys. Herein, we characterized germ cells in macaque testes at 6 months (M), 18 M and 60 M of age, and effectively isolated the spermatogenic cells (including the spermatogonia) from macaque testes with high purity (over 80%) using combined approaches of STA‐PUT separation, Percoll gradients and differential plating. We also generated recipient monkey testes with ablated endogenous spermatogenesis using the alkylating agent busulfan in six macaques, and successfully mimicked autologous cell transplantation in the testes under ultrasonographic guidance. The use of trypan blue led to successful intratubular injection in 4 of 4 testes. Although SSCs in culture showed no significant propagation, we were able to maintain monkey testicular germ cells with stem cell characteristics for up to 3 weeks. Collectively, these data provided meaningful information for future fertility preservation and SSC studies on both non‐human primates and humans.

show abstract

Section: Resultsmentioning

confidence: 99%

Primary culture of germ cells that portray stem cell characteristics and recipient preparation for autologous transplantation in the rhesus monkey

Yuan

Sun

Wang

et al. 2022

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…Consequently, busulfan treatment is the most common method used to prepare recipients of spermatogonial stem cells transplantation [27,28] and to study spermatogonial stem cells kinetics and fertility recovery [29,30,31]. Busulfan can eliminate almost all endogenous germ cells in the recipient, creating an empty space in the spermatogonial stem cells niche; therefore, this drug has been used successfully to prepare recipients in mice [32,33], rats [34,35], pigs [36,37], dogs [38], hamsters [39], monkeys [40], and roosters [41].…”

Section: Chemotherapymentioning

confidence: 99%

Effects of Chemotherapy and Radiotherapy on Spermatogenesis: The Role of Testicular Immunology

Itoh

Sakabe

2019

IJMS

View full text Add to dashboard Cite

Substantial improvements in cancer treatment have resulted in longer survival and increased quality of life in cancer survivors with minimized long-term toxicity. However, infertility and gonadal dysfunction continue to be recognized as adverse effects of anticancer therapy. In particular, alkylating agents and irradiation induce testicular damage that results in prolonged azoospermia. Although damage to and recovery of spermatogenesis after cancer treatment have been extensively studied, there is little information regarding the role of differences in testicular immunology in cancer treatment-induced male infertility. In this review, we briefly summarize available rodent and human data on immunological differences in chemotherapy or radiotherapy.

show abstract

“…Since PGCs are precursors of sperm, researchers suggested that roosters could be used as recipient for exogenous transfer of genetically-modified PGCs which may improve the germline transmission rate (Trefil et al, 2017 ). Chicken embryos and adult roosters were chemically or physically sterilized to create a surrogate for external PGC donors (Trefil et al, 2006 ; Nakamura et al, 2008 , 2010 ; Ghadimi et al, 2017 ). Nakamura and colleagues partially sterilized chicken embryos by injecting Busulfan into the yolk of fertile eggs before incubation; this led to a significant reduction of endogenous PGCs.…”

Section: State Of the Artmentioning

confidence: 99%

Applications of Gene Editing in Chickens: A New Era Is on the Horizon

Sid

Schusser

2018

Front. Genet.

View full text Add to dashboard Cite

The chicken represents a valuable model for research in the area of immunology, infectious diseases as well as developmental biology. Although it was the first livestock species to have its genome sequenced, there was no reverse genetic technology available to help understanding specific gene functions. Recently, homologous recombination was used to knockout the chicken immunoglobulin genes. Subsequent studies using immunoglobulin knockout birds helped to understand different aspects related to B cell development and antibody production. Furthermore, the latest advances in the field of genome editing including the CRISPR/Cas9 system allowed the introduction of site specific gene modifications in various animal species. Thus, it may provide a powerful tool for the generation of genetically modified chickens carrying resistance for certain pathogens. This was previously demonstrated by targeting the Trp38 region which was shown to be effective in the control of avian leukosis virus in chicken DF-1 cells. Herein we review the current and future prospects of gene editing and how it possibly contributes to the development of resistant chickens against infectious diseases.

show abstract

Different approaches to establish infertile rooster

Cited by 6 publications

References 36 publications

Primary culture of germ cells that portray stem cell characteristics and recipient preparation for autologous transplantation in the rhesus monkey

Primary culture of germ cells that portray stem cell characteristics and recipient preparation for autologous transplantation in the rhesus monkey

Effects of Chemotherapy and Radiotherapy on Spermatogenesis: The Role of Testicular Immunology

Applications of Gene Editing in Chickens: A New Era Is on the Horizon

Contact Info

Product

Resources

About