Different Classes of Phytohormones Act Synergistically to Enhance the Growth, Lipid and DHA Biosynthetic Capacity of Aurantiochytrium sp. SW1

Nazir, Yusuf; Halim, Hafiy; Prabhakaran, Pranesha; Ren, Xiaojie; Naz, Tahira; Mohamed, Hassan; Nosheen, Shaista; Mustafa, Kiren; Yang, Weiren; Hamid, Aidil Abdul; Song, Yuanda

doi:10.3390/biom10050755

Cited by 28 publications

(18 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Oleaginous fungi are recognized worldwide as potential sources of PUFA with significant commercial value, such as omega 3 and omega 6 PUFAs [ 6 , 7 ]. The dietary importance of these PUFAs in maintaining human health has been suggested decades ago [ 8 ]. M. circinelloides is a well-known filamentous fungus, considered to be the microbial factory for the production of useful natural compounds and metabolites [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

“…M. circinelloides is a well-known filamentous fungus, considered to be the microbial factory for the production of useful natural compounds and metabolites [ 9 ]. They have the capability of producing a high amount of PUFAs rich in GLA (γ-linolenic acid, 18:3, delta-6,9,12) [ 8 , 10 ]. GLA is an important omega 6 fatty acid that has a crucial role in upholding human well-being and preventing various health-related issues [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11

et al. 2021

Self Cite

View full text Add to dashboard Cite

Background Mucor circinelloides WJ11 is a high-lipid producing strain and an excellent producer of γ-linolenic acid (GLA) which is crucial for human health. We have previously identified genes that encode for AMP-activated protein kinase (AMPK) complex in M. circinelloides which is an important regulator for lipid accumulation. Comparative transcriptional analysis between the high and low lipid-producing strains of M. circinelloides showed a direct correlation in the transcriptional level of AMPK genes with lipid metabolism. Thus, the role of Snf-β, which encodes for β subunit of AMPK complex, in lipid accumulation of the WJ11 strain was evaluated in the present study. Results The results showed that lipid content of cell dry weight in Snf-β knockout strain was increased by 32 % (from 19 to 25 %). However, in Snf-β overexpressing strain, lipid content of cell dry weight was decreased about 25 % (from 19 to 14.2 %) compared to the control strain. Total fatty acid analysis revealed that the expression of the Snf-β gene did not significantly affect the fatty acid composition of the strains. However, GLA content in biomass was increased from 2.5 % in control strain to 3.3 % in Snf-β knockout strain due to increased lipid accumulation and decreased to 1.83 % in Snf-β overexpressing strain. AMPK is known to inactivate acetyl-CoA carboxylase (ACC) which catalyzes the rate-limiting step in lipid synthesis. Snf-β manipulation also altered the expression level of the ACC1 gene which may indicate that Snf-β control lipid metabolism by regulating ACC1 gene. Conclusions Our results suggested that Snf-β gene plays an important role in regulating lipid accumulation in M. circinelloides WJ11. Moreover, it will be interesting to evaluate the potential of other key subunits of AMPK related to lipid metabolism. Better insight can show us the way to manipulate these subunits effectively for upscaling the lipid production. Up to our knowledge, it is the first study to investigate the role of Snf-β in lipid accumulation in M. circinelloides.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…In the present study, the synergistic effect of the C/N ratio, cerulenin, and ketoconazole was checked and optimized for superior β -carotene production by CBS 277.49, using RSM-CCD. RSM is a powerful statistical tool that has been used successfully in many biological and chemical processes for optimization [ 46 ]. It creates an experimental design with a minimal number of experiments.…”

Section: Resultsmentioning

confidence: 99%

Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49

Naz

Nazir

Nosheen

et al. 2020

BioMed Research International

Self Cite

View full text Add to dashboard Cite

Carotenoids produced by microbial sources are of industrial and medicinal importance due to their antioxidant and anticancer properties. In the current study, optimization of β-carotene production in M. circinelloides strain 277.49 was achieved using response surface methodology (RSM). Cerulenin and ketoconazole were used to inhibit fatty acids and the sterol biosynthesis pathway, respectively, in order to enhance β-carotene production by diverting metabolic pool towards the mevalonate pathway. All three variables used in screening experiments were found to be significant for the production of β-carotene. The synergistic effect of the C/N ratio, cerulenin, and ketoconazole was further evaluated and optimized for superior β-carotene production using central composite design of RSM. Our results found that the synergistic combination of C/N ratios, cerulenin, and ketoconazole at different concentrations affected the β-carotene productions significantly. The optimal production medium (std. order 11) composed of C/N 25, 10 μg/mL cerulenin, and 150 mg/L ketoconazole, producing maximum β-carotene of 4.26 mg/L (0.43 mg/g) which was 157% greater in comparison to unoptimized medium (1.68 mg/L, 0.17 mg/g). So, it was concluded that metabolic flux had been successfully redirected towards the mevalonate pathway for enhanced β-carotene production in CBS 277.49.

show abstract

“…Being a very attractive and promising material for the sustainable production of DHA, thraustochytrids are studied extensively in order to improve market prospects. Many different strategies are utilized to enhance and engineer the lipid production of these organisms [ 14 , 15 ]. Of such, impacts of the composition of growth media are vastly studied and literature is profuse about it [ 16 ].…”

Section: Discussionmentioning

confidence: 99%

Two-level factorial analysis of the effect of fructose on DHA biosynthetic capacity of Aurantiochytrium sp. SW1

Manikan

Nazir

Hamid

2021

Heliyon

Self Cite

View full text Add to dashboard Cite

Thraustochytrids are getting increasingly popular due to their high potential role as alternative producers of the high-valued ω-3 polyunsaturated fatty acids (PUFA), docosahexaenoic acid (DHA). While most thraustochytrids prefer glucose as the major carbon source, few strains have been reported to prefer fructose. One such strain is Aurantiochytrium sp. SW1. In this study, the effect of fructose on DHA accumulation by SW1 was investigated using a two-level full factorial design. Besides, biomass, lipid and DHA accumulation profiles of SW1 cultivated in fructose and glucose media were compared. Results revealed that fructose has a very significant positive effect on the volumetric DHA content. Meanwhile, its involvement in affecting DHA biosynthetic capacity, though significant, is not very profound. It was also found that when cultivated in fructose medium, SW1 had a less steep log phase compared to that of glucose medium. However, after 48h of cultivation, biomass and lipid accumulation in fructose medium outweighed the other. Volumetric DHA content in fructose medium at 96h was 11% higher than that of glucose medium. Overall, fructose was found to be a more suitable substrate for biomass, lipid and DHA accumulation in SW1 compared to the conventional source, glucose.

show abstract

Different Classes of Phytohormones Act Synergistically to Enhance the Growth, Lipid and DHA Biosynthetic Capacity of Aurantiochytrium sp. SW1

Cited by 28 publications

References 45 publications

Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11

Role of Snf-β in lipid accumulation in the high lipid‐producing fungus Mucor circinelloides WJ11

Redirecting Metabolic Flux towards the Mevalonate Pathway for Enhanced β-Carotene Production in M. circinelloides CBS 277.49

Two-level factorial analysis of the effect of fructose on DHA biosynthetic capacity of Aurantiochytrium sp. SW1

Contact Info

Product

Resources

About