Different expression patterns of lysosomal proteins exposed to melanin in HeLa cells

Abstract

ed 12 up and 3down regulation spots in relation to melanin exposure. It has been observed that lysoso mal proteins are related to melanin to decrease the color and quantity through cellular activation of lyso somes.

Analysis of lysosomal membrane proteins exposed to melanin in HeLa cells

“…When they grow up on the dishes, 100 ppm of melanin treated on 0, 6, 12, 24, and 48 hours. Each dish was tested toxicity by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay [ 8 ]. The colorimetric MTT metabolic activity assay is applied to determine whether melanin is able to kill cells.…”

“…In other words, previous studies were designed to methods for treating the melanin that has already been generated. Otherwise, some researchers tried to remove, degradation or treatment directly melanin using by extraction [ 5 , 6 ] extracted from plant or enzymes [ 7 , 8 ] isolated from cell organelles or themselves [ 9 ] after generating it. Proteomic analysis is important skill to find and identify unknown proteins.…”

“…So, some researchers who are believed to participate in several functions have conducted a study with respect to treat melanin using by enzymes in lysosome related organelles [ 14 ]. The lysosome is able to extract henʹs egg white [ 7 ] and all of eukaryotes like Saccharomyces cerevisiae [ 14 ] and HeLa cells [ 8 ]. In previous studies, it was conducted research on lysosomal enzymeʹs function.…”