Different fibre populations distinguished by their calcium transient characteristics in enzymatically dissociated murine flexor digitorum brevis and soleus muscles

Calderón, Juan C.; Bolaños, Pura; Torres, Sonia H.; Rodríguez-Arroyo, Greta; Caputo, Carlo

doi:10.1007/s10974-009-9181-1

Cited by 36 publications

(80 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The enzymatic dissociation method is a modification of previously published ones (Bekoff and Betz 1977;Capote et al 2005;Calderón et al 2009Calderón et al , 2010. Briefly, 42-49-day-old male mice (NMRI-IVIC, Navy Medicine Research Institute-Venezuelan Institute for Scientific Research), with a mean weight of 34.4 ± 0.3 g, were killed by rapid cervical dislocation.…”

Section: Fibres Preparationmentioning

confidence: 99%

“…This method is useful in reducing movement artifacts in the tetanic Ca 2? transients (Calderón et al 2009). After loaded, the cells were washed and rested for 20 min to let the AM dyes be fully de-esterified.…”

Section: Fluorescence Recordingsmentioning

confidence: 99%

“…S1 (Calderón et al 2009(Calderón et al , 2010(Calderón et al , 2011. Briefly, fibres types I and IIA share the tetanic Ca 2?…”

Section: Fluorescence Recordingsmentioning

confidence: 99%

“…Interestingly, Ca 2? transients differ depending on the muscle fibre type (Calderón et al 2010) possibly related to the specificity of the proteins related to excitation-contraction coupling (ECC) (Calderón et al 2009(Calderón et al , 2010.…”

Section: Introductionmentioning

confidence: 99%

“…transient). Both morphologies are quite different, but these differences were not further explored (Calderón et al 2009(Calderón et al , 2010.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach

Calderón

Bolaños

Caputo

2014

J Muscle Res Cell Motil

Self Cite

View full text Add to dashboard Cite

One hundred and eighty six enzymatically dissociated murine muscle fibres were loaded with Mag-Fluo-4 AM, and adhered to laminin, to evaluate the effect of modulating cytosolic Ca(2+) buffers and sarcoendoplasmic reticulum Ca(2+) ATPase (SERCA), mitochondria, and Na(+)/Ca(2+) exchanger (NCX) on the differential tetanic Ca(2+) transient kinetics found in different fibre types. Tetanic Ca(2+) transients were classified as morphology type I (MT-I) or type II (MT-II) according to their shape. The first peak of the MT-I (n = 25) and MT-II (n = 23) tetanic Ca(2+) transients had an amplitude (∆F/F) of 0.41 ± 0.03 and 0.83 ± 0.05 and a rise time (ms) of 1.35 and 0.98, respectively. MT-I signals had a time constant of decay (τ1, ms) of 75.9 ± 4.2 while MT-II transients showed a double exponential decay with time constants of decay (τ1 and τ2, ms) of 18.3 ± 1.4 and 742.2 ± 130.3. Sarcoendoplasmic reticulum Ca(2+) ATPase inhibition demonstrated that the decay phase of the tetanic transients mostly rely on SERCA function. Adding Ca(2+) chelators in the AM form to MT-I fibres changed the morphology of the initial five peaks to a MT-II one, modifying the decay phase of the signal in a dose-dependent manner. Mitochondria and NCX function have a minor role in explaining differences in tetanic Ca(2+) transients among fibre types but still help in removing Ca(2+) from the cytosol in both MT-I and MT-II fibres. Cytoplasmic Ca(2+) buffering capacity and SERCA function explain most of the different kinetics found in tetanic Ca(2+) transients from different fibre types, but mitochondria and NCX have a measurable role in shaping tetanic Ca(2+) responses in both slow and fast-twitch muscle fibre types. We provided experimental evidence on the mechanisms that help understand the kinetics of tetanic Ca(2+) transients themselves and explain kinetic differences found among fibre types.

show abstract

Section: Fibres Preparationmentioning

confidence: 99%

Section: Fluorescence Recordingsmentioning

confidence: 99%

“…S1 (Calderón et al 2009(Calderón et al , 2010(Calderón et al , 2011. Briefly, fibres types I and IIA share the tetanic Ca 2?…”

Section: Fluorescence Recordingsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…transient). Both morphologies are quite different, but these differences were not further explored (Calderón et al 2009(Calderón et al , 2010.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach

Calderón

Bolaños

Caputo

2014

J Muscle Res Cell Motil

Self Cite

View full text Add to dashboard Cite

show abstract

The excitation–contraction coupling mechanism in skeletal muscle

2014

View full text Add to dashboard Cite

First coined by Alexander Sandow in 1952, the term excitation-contraction coupling (ECC) describes the rapid communication between electrical events occurring in the plasma membrane of skeletal muscle fibres and Ca 2+ release from the SR, which leads to contraction. The sequence of events in twitch skeletal muscle involves: (1) initiation and propagation of an action potential along the plasma membrane, (2) spread of the potential throughout the transverse tubule system (T-tubule system), (3) dihydropyridine receptors (DHPR)-mediated detection of changes in membrane potential, (4) allosteric interaction between DHPR and sarcoplasmic reticulum (SR) ryanodine receptors (RyR), (5) release of Ca 2+ from the SR and transient increase of Ca 2+ concentration in the myoplasm, (6) activation of the myoplasmic Ca 2+ buffering system and the contractile apparatus, followed by (7) Ca 2+ disappearance from the myoplasm mediated mainly by its reuptake by the SR through the SR Ca 2+ adenosine triphosphatase (SERCA), and under several conditions movement to the mitochondria and extrusion by the Na + /Ca 2+ exchanger (NCX). In this text, we review the basics of ECC in skeletal muscle and the techniques used to study it. Moreover, we highlight some recent advances and point out gaps in knowledge on particular issues related to ECC such as (1) DHPR-RyR molecular interaction, (2) differences regarding fibre types, (3) its alteration during muscle fatigue, (4) the role of mitochondria and store-operated Ca 2+ entry in the general ECC sequence, (5) contractile potentiators, and (6) Ca 2+ sparks.

show abstract

Calibration of mammalian skeletal muscle Ca2+ transients recorded with the fast Ca2+ dye Mag-Fluo-4

Milán

Rincón

Arango

et al. 2021

Biochimica et Biophysica Acta (BBA) - General Subjects

View full text Add to dashboard Cite

Different fibre populations distinguished by their calcium transient characteristics in enzymatically dissociated murine flexor digitorum brevis and soleus muscles

Cited by 36 publications

References 54 publications

Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach

Tetanic Ca2+ transient differences between slow- and fast-twitch mouse skeletal muscle fibres: a comprehensive experimental approach

The excitation–contraction coupling mechanism in skeletal muscle

Calibration of mammalian skeletal muscle Ca2+ transients recorded with the fast Ca2+ dye Mag-Fluo-4

Contact Info

Product

Resources

About