2015
DOI: 10.1016/j.bpj.2015.04.024
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Different KChIPs Compete for Heteromultimeric Assembly with Pore-Forming Kv4 Subunits

Abstract: Auxiliary Kv channel-interacting proteins 1-4 (KChIPs1-4) coassemble with pore-forming Kv4 α-subunits to form channel complexes underlying somatodendritic subthreshold A-type current that regulates neuronal excitability. It has been hypothesized that different KChIPs can competitively bind to Kv4 α-subunit to form variable channel complexes that can exhibit distinct biophysical properties for modulation of neural function. In this study, we use single-molecule subunit counting by total internal reflection fluo… Show more

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Cited by 11 publications
(9 citation statements)
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“…GFP-tagged subunit): one step means a single bleaching event of GFP, indicating the existence of single subunit. It is an especially powerful method for examining stoichiometry because each individual macromolecule complex can be evaluated (counted) (50,51,56,57). Before determining the stoichiometry of the Kv4.2-DPP10 complex, we first determined the stoichiometry of DPP10 alone.…”
Section: Dpp10 Changes Kv42 Properties In An Expression Level-dependmentioning
confidence: 99%
“…GFP-tagged subunit): one step means a single bleaching event of GFP, indicating the existence of single subunit. It is an especially powerful method for examining stoichiometry because each individual macromolecule complex can be evaluated (counted) (50,51,56,57). Before determining the stoichiometry of the Kv4.2-DPP10 complex, we first determined the stoichiometry of DPP10 alone.…”
Section: Dpp10 Changes Kv42 Properties In An Expression Level-dependmentioning
confidence: 99%
“…Loss of one of the KCNIPs has previously been observed to result in an increase in protein expression of the other, non-disrupted KCNIPs [36]. This compensatory mechanism is thought to occur through competition between the KCNIPs for binding to α-subunits [36,37]. The stabilisation that occurs through binding to the other subunits results in an increase in protein levels.…”
Section: Discussionmentioning
confidence: 99%
“…8). This likely involves multiple KChIP4a-dependent steps that include the association of Kv4 We previously reported that different KChIPs, including KID-containing KChIPs and KChIPs without KID, could compete for heteromultimeric assembly with Kv4 αsubunits, which depends on the relative expression level of each KChIP subunit (Zhou et al, 2015). Therefore, although the stoichiometry of the KChIP4a-Hsp70 complex is unclear, a Kv4-KChIPs complex containing even only one KChIP4a molecule may still be recognized by Hsp70-CHIP for degradation.…”
Section: Discussionmentioning
confidence: 99%