Different Kraft lignin sources for electrospun nanostructures production: Influence of chemical structure and composition

García-Fuentevilla, Luisa; Rubio‐Valle, José Fernando; Martín‐Sampedro, Raquel; Valencia, C.; Eugenio, María E.; Ibarra, David

doi:10.1016/j.ijbiomac.2022.06.121

Cited by 24 publications

(9 citation statements)

References 80 publications

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“…They included the whole spectra (δ C /δ H 0.0–150.0/0.0–9.0) in Supplementary Figure S3 , and the spectra corresponding to the oxygenated aliphatic (δ C /δ H 45.0–95.0/2.5–6.0 ppm) and the aromatic (δ C /δ H 90.0–150.0/5.0–9.0 ppm) regions in Figure 7 and Figure 8 , respectively. The main 13 C– 1 H lignin correlation signals identified in HSQC spectra are displayed in Table S3 , endorsed according to those described by the literature [ 42 , 44 , 45 , 46 , 47 ]. The lignin substructures identified are depicted in Figures S4 and S5 .…”

Section: Resultsmentioning

confidence: 56%

“…FTIR spectra of untreated and laccase-treated kraft lignins (MtL-KL and SiLA-KL resulting from experiments with maximum decrease in phenolic content and increase in molecular weight achieved) are displayed in Figure 6 . The observed bands were assigned in comparison with others previously reported in the literature [ 41 , 42 ] and are displayed in Table S2 . FTIR spectrum of kraft lignin showed the characteristic bands of lignin, which include those observed at 1610, 1515 and 1415 cm −1 associated with aromatic ring vibrations, and at 1455 cm −1 attributed to C−H asymmetric vibrations and deformations ( Figure 6 a).…”

Section: Resultsmentioning

confidence: 65%

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Enzyme-Catalyzed Polymerization of Kraft Lignin from Eucalyptus globulus: Comparison of Bacterial and Fungal Laccases Efficacy

et al. 2023

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Kraft lignin, a side-stream from the pulp and paper industry, can be modified by laccases for the synthesis of high added-value products. This work aims to study different laccase sources, including a bacterial laccase from Streptomyces ipomoeae (SiLA) and a fungal laccase from Myceliophthora thermophila (MtL), for kraft lignin polymerization. To study the influence of some variables in these processes, a central composite design (CCD) with two continuous variables (enzyme concentration and reaction time) and three levels for each variable was used. The prediction of the behavior of the output variables (phenolic content and molecular weight of lignins) were modelled by means of response surface methodology (RSM). Moreover, characterization of lignins was performed by Fourier-transform infrared (FTIR) spectroscopy and different nuclear magnetic resonance (NMR) spectroscopy techniques. In addition, antioxidant activity was also analyzed. Results showed that lignin polymerization (referring to polymerization as lower phenolic content and higher molecular weight) occurred by the action of both laccases. The enzyme concentration was the most influential variable in the lignin polymerization reaction within the range studied for SiLA laccase, while the most influential variable for MtL laccase was the reaction time. FTIR and NMR characterization analysis corroborated lignin polymerization results obtained from the RSM.

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Section: Resultsmentioning

confidence: 56%

Section: Resultsmentioning

confidence: 65%

Enzyme-Catalyzed Polymerization of Kraft Lignin from Eucalyptus globulus: Comparison of Bacterial and Fungal Laccases Efficacy

et al. 2023

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“…We estimated the amounts of β-O-4′, phenylcoumaran, and resinol by analyzing the volumes of their C α -H α correlation peaks. , Similarly, the amount of diaresinol was estimated from the volume of the C β -H β correlation peak. To estimate the amount of xylan, we analyzed the volume of the C 3 –H 3 correlation peak.…”

Section: Methodsmentioning

confidence: 99%

Understanding Manganese Peroxidase-Catalyzed Conversion of the Lignin Structure and Its Application for the Polymerization of Kraft Lignin

Teo,

Kondo,

Watanabe

et al. 2024

ACS Sustainable Chem. Eng.

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Manganese peroxidase (MnP) has been well studied for woody biomass degradation. MnP exhibits a biotechnological potential for producing lignin-based materials through Kraft lignin (KL) polymerization. MnP derived from Ceriporiopsis subvermispora (CsMnP) is particularly intriguing as this fungus predominantly utilizes MnP to degrade/modify lignin. Here, we investigated CsMnP's catalytic activity toward the phenolic β-O-4′ lignin substructure, utilizing guaiacylglycerol-βguaiacyl ether (GGE) as the model substrate. The reaction carried out at 25 °C and pH 5 was monitored by RP-HPLC, leading to the isolation of five product peaks (P1 to P5) after 48 h. SEC analysis indicated that compounds in P1 to P5 had higher molecular weights than GGE, suggesting polymerization reactions. NMR analysis of P1 revealed that this compound contains two GGE segments, connected by a 5-5′ linkage. Furthermore, we demonstrated CsMnP's ability to modify KL at 25 °C and pH 5, yielding a product with a 360% higher molecular weight compared to untreated KL after 24 h. NMR spectra revealed the deprotonation of the benzenic ring in KL and polymerization through the possible formation of α-5′, 5-5′, and 4-O-5′ linkages. This study offers valuable insights into the enzymatic properties of CsMnP and presents a potential strategy for valorizing KL.

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“…PLCL (BIOMQ, China) and ADM (Marine Biomedical Research Institute of Qingdao) was dissolved in HFIP (Chembee, China) at various ratios (10:0, 7:3, 5:5, 3:7, and 0:10) (w/w) and stirred overnight to obtain homogeneous solutions of 10% (w/v). The nanofiber composition ratio was determined based on the previous research methods [41][42][43][44][45][46][47] and the preliminary evaluation results of this study for the nanofiber composition ratio (figure S1). When electrospinning, the flow rate was 3 ml h −1 , and the voltage was set to 13 kV.…”

Section: Preparation Of the Plcl/adm Nanofiber Scaffoldsmentioning

confidence: 99%

Promotion of adipose stem cell transplantation using GelMA hydrogel reinforced by PLCL/ADM short nanofibers

Ning,

Liu,

Sun

et al. 2023

Biomed. Mater.

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Adipose-derived mesenchymal stem cells (ADSCs) show poor survival after transplantation, limiting their clinical application. In this study, a series of poly(l-lactide-co-ϵ-caprolactone) (PLCL)/acellular dermal matrix (ADM) nanofiber scaffolds with different proportions were prepared by electrospinning. By studying their morphology, hydrophilicity, tensile mechanics, and biocompatibility, PLCL/ADM nanofiber scaffolds with the best composition ratio (PLCL:ADM = 7:3) were selected to prepare short nanofibers. And based on this, injectable gelatin methacryloyl (GelMA) hydrogel loaded with PLCL/ADM short nanofibers (GelMA-Fibers) was constructed as a transplantation vector of ADSCs. ADSCs and GelMA-Fibers were co-cultured, and the optimal loading concentration of PLCL/ADM nanofibers was investigated by cell proliferation assay, live/dead cell staining, and cytoskeleton staining in vitro. In vivo investigations were also performed by H&E staining, Oil red O staining, and TUNEL staining, and the survival and apoptosis rates of ADSCs transplanted in vivo were analyzed. It was demonstrated that GelMA-Fibers could effectively promote the proliferation of ADSCs in vitro. Most importantly, GelMA-Fibers increased the survival rate of ADSCs transplantation and decreased their apoptosis rate within 14 d. In conclusion, the constructed GelMA-Fibers would provide new ideas and options for stem cell tissue engineering and stem cell-based clinical therapies.

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Different Kraft lignin sources for electrospun nanostructures production: Influence of chemical structure and composition

Cited by 24 publications

References 80 publications

Enzyme-Catalyzed Polymerization of Kraft Lignin from Eucalyptus globulus: Comparison of Bacterial and Fungal Laccases Efficacy

Enzyme-Catalyzed Polymerization of Kraft Lignin from Eucalyptus globulus: Comparison of Bacterial and Fungal Laccases Efficacy

Understanding Manganese Peroxidase-Catalyzed Conversion of the Lignin Structure and Its Application for the Polymerization of Kraft Lignin

Promotion of adipose stem cell transplantation using GelMA hydrogel reinforced by PLCL/ADM short nanofibers

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