Different Modulating Effects of the Monoamines Adrenaline, Noradrenaline, and Serotonin on the DNA Synthesis Response of Human Peripheral Blood T Lymphocytes Activated by Mercuric Chloride and Nickel Sulfate

Abstract

The influence of adrenaline, noradrenaline, and serotonin (5-hydroxytryptamine) was tested on the proliferative response of peripheral blood T lymphocytes of nickel-allergic subjects to mercuric chloride and nickel sulfate. With addition of the monoamines 1 h after mercuric chloride, there was a marked suppression with all the substances at 10^––⁴M, while adrenaline also inhibited down to a concentration of 10^––5M and serotonin down to 10^––7M. At 3 days after mercuric chloride, adrenaline inhibited at 10^––4M and serotonin also down to a concentration of 10^––5M. With addition at 1 h after nickel sulfate, there was an inhibition by all the monoamines at 10^––4M, while, in contrast, adrenaline at 10^––6 to 10^––8M and serotonin at 10^––5 to 10^––6M gave a stimulation of the response. These findings point at different modulating effects on the proliferative response induced by a polyclonal activator such as mercuric chloride and a monoclonal activator such as nickel sulfate.

Inhibiting Effects of Serotonin Antagonists on the Proliferation of Mercuric Chloride Stimulated Human Peripheral Blood T Lymphocytes

“…The substances were dissolved in 0.9% saline to give final concentrations in the cell cultures from 10"4 to 10~l(l mol/1 for ketanserin and methiotepine and of 10-' mol/1 for 5-HT [11],…”

“…When the influence of 5-HT on the proliferative response of human peripheral blood T lymphocytes to mercuric chloride [11], a polyclonal stimulator of human and animal lymphoid cells [12], was tested, an inhibited response was obtained down to a 5-HT concentration of 10~7 mol/1.…”

Reciprocal modulation of helper Th1 and Th17 cells by the β2‐adrenergic receptor agonist drug terbutaline

“…We observed a small but significant proliferative effect on Th cells activated and treated with TERB. An antiproliferation effect on T cells has been reported with various adrenergic agonists such as isoproterenol, fenoterol, NE, and the inflammatory mediator PGE 2 , which was among the strongest proliferation inhibitors [43][44][45][46][47][48][49][50]. However, TERB is relatively weak in comparison with these agonists [51].…”