Different natural organic matter isolates cause similar stress response patterns in the freshwater amphipod, Gammarus pulex

Bedulina, Daria; Timofeyev, Maxim A.; Zimmer, Martin; Zwirnmann, Elke; Menzel, Ralph; Steinberg, Christian E. W.

doi:10.1007/s11356-009-0222-5

Cited by 20 publications

(7 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Whether or not this finding is generalizable should be checked with usual animals in sediment toxicity tests, namely amphipods, mussels, and fishes. Nevertheless, this study confirms the findings of other studies on direct toxic effects of humic substances on nematodes, cladocerans, amphipods, and fishes (Höss et al 2001;Meinelt et al 2007;Steinberg et al 2007Steinberg et al , 2010Bedulina et al 2010;Bouchnak and Steinberg 2010). Although organic matter might be one source of stress, it is a necessary component of formulated control sediments, to mimic natural conditions.…”

Section: Resultssupporting

confidence: 90%

“…Consequently, it is assumed that the SOM itself acted as natural xenobiotics in relation to their interactions with the biota-in analogy to secondary plant compounds (Li et al 2007); yet, in contrast to the latter, HSs are not synthesized to defend against herbivory, but challenge any exposed organism (Timofeyev et al 2004(Timofeyev et al , 2006Menzel et al 2005b;Bedulina et al 2010). Indeed, HSs as the major part of P have a variety of functional groups, which resemble xenobiotic chemicals.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Organic carbon source in formulated sediments influences life traits and gene expression of Caenorhabditis elegans

et al. 2011

Self Cite

View full text Add to dashboard Cite

River water quality is strongly influenced by their sediments and their associated pollutants. To assess the toxic potential of sediments, sediment toxicity tests require reliable control sediments, potentially including formulated control sediments as one major option. Although some standardization has been carried out, one critical issue still remains the quality of sediment organic matter (SOM). Organic carbon not only binds hydrophobic contaminants, but may be a source of mild toxicity, even if the SOM is essentially uncontaminated. We tested two different sources of organic carbon and the mixture of both (Sphagnum peat (P) and one commercial humic substances preparation-HuminFeed(®), HF) in terms of life trait variables and expression profiles of selected life performance and stress genes of the nematode Caenorhabditis elegans. In synchronous cultures, gene expression profiling was done after 6 and 48 h, respectively. The uncontaminated Sphagnum P reduced growth, but increased numbers of offspring, whereas HF did not significantly alter life trait variables. The 6 h expression profile showed most of the studied stress genes repressed, except for slight to strong induction in cyp-35B1 (all exposures), gst-38 (only mixture), and small hsp-16 genes (all exposures). After 48 h, the expression of almost all studied genes increased, particularly genes coding for antioxidative defense, multiple xenobiotic resistance, vitellogenin-like proteins, and genes regulating lifespan. Overall, even essentially uncontaminated SOM may induce several modes of action on the molecular level in C. elegans which may lead to false results if testing synthetic xenobiotics. This contribution is a plea for a strict standardization of the SOM quality in formulated sediments and to check for corresponding effects in other model sediment organisms, especially if using molecular toxicity endpoints.

show abstract

Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Organic carbon source in formulated sediments influences life traits and gene expression of Caenorhabditis elegans

et al. 2011

Self Cite

View full text Add to dashboard Cite

show abstract

“…Humic acids can be integrated into cells and, as shown for freshwater amphipods, can induce stress defence mechanisms (Steinberg et al ., 2006; Bedulina et al ., 2010). RB may diffuse through cellular membranes (DeRosa and Crutchley, 2002) and thereby mimics the non‐specific incorporation of humic acids into living cells.…”

Section: Discussionmentioning

confidence: 99%

Singlet oxygen, a neglected but important environmental factor: short‐term and long‐term effects on bacterioplankton composition in a humic lake

Glaeser

Grossart

Glaeser

2010

Environmental Microbiology

View full text Add to dashboard Cite

Photolysis of dissolved organic matter (DOM) leads to contrasting effects on bacterioplankton dynamics, i.e. stimulation and inhibition of bacterial activity. In particular, the role of short-lived reactive oxygen species (ROS), e.g. singlet oxygen (¹O₂), in altering microbial activity and species composition has scarcely been investigated. Therefore, we have artificially increased the natural rate of ¹O₂ formation in short-term (∼4 h) in situ and long-term (72 h) laboratory incubations of surface water samples from a humic acid-rich lake. Denaturing gradient gel electrophoresis (DGGE) patterns revealed significant changes in occurrence of abundant bacterioplankton phylotypes upon ¹O₂ exposure. Cluster analysis of DGGE patterns showed that a moderate increase in ¹O₂ exposure leads to similar changes in different years indicating the establishment of bacterial communities adapted to ¹O₂ exposure. Bacterioplankton phylotypes favoured under these conditions belonged to Betaproteobacteria of the beta II cluster (e.g. Polynucleobacter necessarius) and the beta I cluster related to Limnohabitans (R-BT subcluster) as well as Alphaproteobacteria affiliated to Novosphingobium acidiphilum. In contrast, Actinobacteria of the freshwater acI-B cluster were sensitive even against moderate ¹O₂ exposure. We conclude that ¹O₂ exposure due to DOM photolysis represents an important natural selective factor affecting bacterial species dynamics in aquatic ecosystems in many ways.

show abstract

“…Methodologies are available for measuring a total of 21 biomarkers, such as the metallothionein-like proteins, indicative of metal exposure (Stuhlbacher and Maltby, 1992;Correia et al, 2001;Geffard et al, 2007;Gismondi et al, 2012), acetylcholinesterase activity (McLoughlin et al, 2000;Xuereb et al, 2007Xuereb et al, , 2009a related to pesticide exposure, and neurotoxicity, the phase II xenobiotic transformation enzyme (glutathione S-transferase, glutathione peroxidase, catalase, and superoxide dismutase) (Maltby and Hills, 2008;Bedulina et al, 2010a;Turja et al, 2014), which signal detoxification activity. Effective biomarkers determined in Gammarus genus include catalase (Sroda and Cossu-Leguille, 2011), total glutathione peroxidase (Turja et al, 2014), peroxidase (Bedulina et al, 2010b), and superoxide dismutase (Turja et al, 2014) activities, all indicative of antioxidative defense induction; the increase of heat shock protein from different families (Schirling et al, 2006;Scheil et al, 2008;Bedulina et al, 2010a), related to the general stress response; changes in digestive enzymes including amylase, cellulase, endoglucanase, esterase, trypsin, β-galactosidase, and β-glucosidase (Dedourge- Geffard et al, 2009;Charron et al, 2013); and, finally, activity of the sodium pump Na + /K + adenosine triphosphatase (Felten et al, 2008a,b;Issartel et al, 2010) linked to alteration of iono-osmoregulation. Three-quarters of the studies on biomarkers have been conducted under laboratory conditions in the context of environmental risk assessment of chemical contamination and very few in field surveys (Crane et al, 1995;Maltby and Hills, 2008;Sanchez and Porcher, 2009).…”

Section: Molecular Markersmentioning

confidence: 99%

Gammarids as Reference Species for Freshwater Monitoring

Chaumot¹,

Geffard²,

Armengaud³

et al. 2015

Aquatic Ecotoxicology

View full text Add to dashboard Cite

Different natural organic matter isolates cause similar stress response patterns in the freshwater amphipod, Gammarus pulex

Abstract: Effect studies of chemical stresses on organisms should consider exposure to both natural triggers and xenobiotic compounds in low concentrations--in order to prospectively differentiate between these triggers and, subsequently, classify them.

Cited by 20 publications

References 44 publications

Organic carbon source in formulated sediments influences life traits and gene expression of Caenorhabditis elegans

Organic carbon source in formulated sediments influences life traits and gene expression of Caenorhabditis elegans

Singlet oxygen, a neglected but important environmental factor: short‐term and long‐term effects on bacterioplankton composition in a humic lake

Gammarids as Reference Species for Freshwater Monitoring

Contact Info

Product

Resources

About