Different regulations of cell-type transcription by UV-B in multicellular green alga Volvox carteri

Abstract

There is a scarcity of research reports on the effect of ultraviolet (UV)-B radiation on genome-wide transcriptional regulation in the multicellular green microalga including Volvox carteri (V. carteri). This microalga possesses only two cell types including mortal and motile somatic cells, as well as immortal and immotile reproductive cells. Therefore, the present study evaluated the effect of low-dose UV-B radiation on the cell-type-specific gene expression pattern of reproductive and somatic cells in an asexual life cycle of V. carteri using RNA sequence method. To this end, the separated reproductive and somatic cells were treated for 1 hour at an intensity of 0.056 mW/cm −2 UV-B radiation. Then, a transcriptome analysis was conducted between the UV-B and white light treated groups in either of the cell types. Based on differential gene expression analyses, no differentially expressed genes were found in reproductive cells under the treatment as compared to the control group. This type of cell maintained its steady state. However, treating the somatic cells with UV-B radiation led to at least 126 differentially expressed genes compared to the untreated control group. In addition, the results of a direct comparison demonstrated a restricted and wide response to UV-B radiation in somatic cells as compared to reproductive cells. Based on the results, UV-B radiation could be involved in cell-typespecific regulation of biological pathways.

Integrated analysis of the physiological, transcriptomic and metabolomic responses of Neoporphyra haitanensis after exposure to UV-B radiation: an energy metabolism perspective

“…To defend against the negative effects of UV-B radiation stress, plants have evolved various adaptative and repair mechanisms, which can play crucial roles in balancing growth and stress responses. These adaptations manifest themselves in terms of changes in plant morphology, physiological characteristics (Yang et al, 2004(Yang et al, , 2005, biochemical parameters (Kumari et al, 2010;Tripathi et al, 2011) and gene expression levels (Ekhtari et al, 2019). For example, in order to cope with UV-B stress, plants produce protective compounds that remodel the metabolism pathway to serve the energy demand under stress (Sun Y et al, 2022).…”