Different responses of biochemical markers in frogs (Rana ridibunda) from urban and rural wetlands to the effect of carbamate fungicide

Falfushinska, Halina; Романчук, Л. Д.; Stolyar, Oksana

doi:10.1016/j.cbpc.2008.05.018

Cited by 26 publications

(16 citation statements)

References 46 publications

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“…Exposure to nominal concentrations of Tattoo of 3, 5, or 10 mg L −1 caused a significant enhancement of CP levels in liver and kidney by 92%–125% and 98%–111%, respectively, but did not affect brain CP content. These results are in a good agreement with the data of Falfushinska et al () who reported similar protein damage in livers of Tattoo‐treated frogs. A more marked Tattoo influence on CP levels was observed in frog populations from a polluted site relative to a reference one.…”

Section: Resultssupporting

confidence: 92%

“…(A)], whereas in brain, the activity of SOD was not changed by fungicide exposure. Recently, an increase Cu,Zn‐SOD activity in frog liver in response to carbamate fungicide treatment was demonstrated (Falfushinska et al,). This may be explained by the release of manganese and zinc ions during Mancozeb catabolism or its interaction with metal‐containing enzymes (Vettorazzi et al,), particularly SOD.…”

Section: Resultsmentioning

confidence: 99%

“…The toxic effects of Mancozeb were reported to be fairly low for cell cultures of rat fibroblasts (Calviello et al,) and in frogs (Falfushinska et al,), and oxidative stress‐related mechanisms of Mancozeb toxicity have been suggested (Calviello et al,). There is some evidence of prooxidant actions of Tattoo (Mancozeb) (Rath et al,).…”

Section: Introductionmentioning

confidence: 99%

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The mancozeb-containing carbamate fungicide tattoo induces mild Oxidative Stress in goldfish brain, liver, and kidney

et al. 2013

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Tattoo belongs to the group of carbamate fungicides and contains Mancozeb (ethylene(bis)dithiocarbamate) as its main constituent. The toxicity of Mancozeb to living organisms, particularly fish, is not resolved. This work investigated the effects of 96 h of exposure to 3, 5, or 10 mg L(-1) of Tattoo (corresponding to 0.9, 1.5, or 3 mg L(-1) of Mancozeb) on the levels of oxidative stress markers and the antioxidant enzyme system of brain, liver, and kidney of goldfish, Carassius auratus). In liver, Tattoo exposure resulted in increased activities of superoxide dismutase (SOD) by 70%-79%, catalase by 23%-52% and glutathione peroxidase (GPx) by 49%. The content of protein carbonyls (CP) in liver was also enhanced by 92%-125% indicating extensive damage to proteins. Similar increases in CP levels (by 98%-111%) accompanied by reduced glucose-6-phosphate dehydrogenase activity (by 13%-15%) was observed in kidney of fish exposed to Tattoo; however, SOD activity increased by 37% in this tissue after treatment with 10 mg L(-1) Tattoo. In brain, a rise in lipid peroxide level (by 29%) took place after exposure to 10 mg L(-1) Tattoo and was accompanied by elevation of high-molecular mass thiols (by 14%). Tattoo exposure also resulted in a concentration-dependent decrease in glutathione reductase activity (by 26%-37%) in brain. The data collectively show that exposure of goldfish to 3-10 mg L(-1) of the carbamate fungicide Tattoo resulted in the development of mild oxidative stress and activation of antioxidant defense systems in goldfish tissues.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

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The mancozeb-containing carbamate fungicide tattoo induces mild Oxidative Stress in goldfish brain, liver, and kidney

et al. 2013

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“…Such an analysis was used recently, for instance, in frogs (Rana ridibunda) collected from sites polluted with carbamate fungicides [77], in Chinese hamster fibroblasts treated with the fungicide thiram [78], in rat interstitial cells treated with the insecticide dimethoate [79] and in various rat tissues treated with the insecticide fenvalerate [80].…”

Section: Pesticidesmentioning

confidence: 99%

Linking protein oxidation to environmental pollutants: Redox proteomic approaches

Braconi

Bernardini

Santucci

2011

Journal of Proteomics

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“…Assessment of biological effects on animal species is frequently employed to monitor contaminations [5]. The use of sensor organisms reveals the status of the dynamic scenario of environmental contamination and their biological effect [4].…”

Section: Introductionmentioning

confidence: 99%

Nuclear Abnormalities in Erythrocytes of Frogs from Wetlands and Croplands of Western Ghats Indicate Environmental Contaminations

Raghunath

Veerabhadrappa²,

Krishnamurthy

2017

J.Trop.Life.Science

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Anuran amphibians are the biological models to assess the influence of environmental contamination. We conducted nuclear abnormality assessment and micronuclei test in erythrocytes of frogs to identify an early influence of environmental contaminations. In Western Ghats of India, farmers use different agrochemicals and obviously, the amphibian habitat is contaminated with combinations of many residues. Many frog species use these agro-ecosystem for breeding and to complete early life stage. In the present study, we used Indian skipper frog (Euphlyctis cyanophlyctis (Anura: Ranidae)), a common inhabitant of water bodies in agro-ecosystems of Western Ghats for the assay. We collected the adult frogs from three different habitats; a) the rice paddy fields contaminated with agrochemicals, b) uncontaminated marsh and c) a shallow water pool. We recorded micronucleus, blebbed, lobed, kidney bean shaped nucleus and nucleus with notches in erythrocytes of these frogs. These nuclear abnormalities in erythrocytes are quantified. The incidences of occurrence of micronucleus ranged from 0 to 8 in 1000 analysed cells. More than 80% of the frogs living in agro-chemical contaminated site showed these abnormalities. The appearance of nuclear abnormalities indicates that the frogs are affected by agro-chemicals.

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Different responses of biochemical markers in frogs (Rana ridibunda) from urban and rural wetlands to the effect of carbamate fungicide

Cited by 26 publications

References 46 publications

The mancozeb-containing carbamate fungicide tattoo induces mild Oxidative Stress in goldfish brain, liver, and kidney

The mancozeb-containing carbamate fungicide tattoo induces mild Oxidative Stress in goldfish brain, liver, and kidney

Linking protein oxidation to environmental pollutants: Redox proteomic approaches

Nuclear Abnormalities in Erythrocytes of Frogs from Wetlands and Croplands of Western Ghats Indicate Environmental Contaminations

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