Different Sarcocystis spp. are present in bovine eosinophilic myositis

Vangeel, Lieve; Houf, Kurt; Geldhof, Peter; Preter, Katleen De; Vercruysse, Jozef; Ducatelle, Richard; Chiers, Koen

doi:10.1016/j.vetpar.2013.06.001

Cited by 51 publications

(58 citation statements)

References 23 publications

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“…In these species from cervid intermediate hosts, the maximum intraspecific sequence divergence, when comparing the near full-length gene, has varied between 0.6 and 1.9 %. Vangeel et al (2013) reported a much higher divergence in S. hominis from cattle (up to 5.5 %), but they cloned and compared only a short fragment (about 160 bp) of the gene, comprising many variable sites. The well-documented intraspecific heterogeneity in the 18S rRNA gene of several Sarcocystis spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterisation of three regions of the nuclear ribosomal DNA unit and the mitochondrial cox1 gene of Sarcocystis fusiformis from water buffaloes (Bubalus bubalis) in Egypt

2015

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A total of 33 macroscopically visible (3-11 × 1-5 mm) sarcocysts of Sarcocystis fusiformis were excised from the oesophagus of 12 freshly slaughtered water buffalos in Giza, Egypt. Genomic DNA was extracted from the sarcocysts, and all isolates were characterised at the mitochondrial cytochrome c oxidase subunit I (cox1) gene through PCR amplification and direct sequencing, whereas a few selected isolates were characterised at the 18S and 28S ribosomal (r) RNA genes and the internal transcribed spacer 1 (ITS1) region of the nuclear rDNA unit following cloning. Among the 33 cox1 sequences (1,038-bp long), there was a total of 13 haplotypes, differing from each other by one to seven substitutions and sharing an identity of 99.3-99.9 %. In comparison, the sequence identity was 98.8-99.0 % among eight complete 18S rRNA gene sequences (1,873-1,879-bp long), 98.1-100 % among 28 complete ITS1 sequences (853-864-bp long) and 97.4-99.6 % among five partial 28S rRNA gene sequences (1,607-1,622 bp). At the three nuclear loci, the intraspecific (and intra-isolate) sequence variation was due to both substitutions and indels, which necessitated cloning of the PCR products before sequencing. Some additional clones of the 18S and 28S rRNA genes were highly divergent from the more typical clones, but the true nature of these aberrant clones could not be determined. Sequence comparisons and phylogenetic analyses based on either 18S rRNA gene or cox1 nucleotide sequences, placed S. fusiformis closest to Sarcocystis cafferi from the African buffalo, but only the analyses based on cox1 data separated the two taxa clearly from each other and showed that they were separate species (monophyletic clusters and 93 % sequence identity at cox1 versus interleaved sequences and 98.7-99.1 % sequence identity at the 18S rRNA gene). Two cats experimentally infected with sarcocysts of S. fusiformis started shedding small numbers of sporocysts 8-10 days post-infection (dpi) and were euthanized 15 dpi. Sporocysts isolated from the intestinal mucosa of both cats were identified molecularly as belonging to S. fusiformis through PCR amplification and sequencing of the partial cox1. The two sporocyst-derived cox1 sequences were identical with the most common sarcocyst-derived cox1 haplotype.

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Section: Discussionmentioning

confidence: 99%

Molecular characterisation of three regions of the nuclear ribosomal DNA unit and the mitochondrial cox1 gene of Sarcocystis fusiformis from water buffaloes (Bubalus bubalis) in Egypt

2015

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“…S. hominis has public health importance, causing gastrointestinal malaise [ 1 ], but “ S. sinensis ” may also elicit symptoms in humans [ 4 ] and therefore can be regarded as potentially zoonotic. Bovine sarcocystiosis was reported to entail eosinophilic myositis [ 5 ], encephalomyelitis [ 6 ] and acute death of cattle (so-called “Dalmeny-disease” caused by S. cruzi [ 7 , 8 ]).…”

Section: Introductionmentioning

confidence: 99%

Sarcocystis-infection of cattle in Hungary

et al. 2015

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BackgroundReports on Sarcocystis-infection of cattle are outdated or lacking in many European countries, including those in the Central-Eastern part of the continent. Therefore, to assess the prevalence of Sarcocystis spp. among bovids in Hungary, a countrywide survey was initiated. In addition, fulminant deaths of four cattle, that showed clinical signs and post mortem lesions resembling acute sarcocystiosis (“Dalmeny disease”), were investigated.MethodsDuring the countrywide survey individual heart and oesophagus samples were collected at slaughterhouses from 151 beef cattle and from 15 buffalo, kept in 31 places of Hungary. Analysis for Sarcocystis spp. was carried out with conventional PCRs for the 18S rDNA gene and gel electrophoresis, followed by sequencing of 36 strongly positive samples. Mortality cases were evaluated by histological, molecular, bacteriological and virological analyses of samples from various organs.ResultsAmong slaughtered cattle the rate of Sarcocystis-infection was 66%. S. cruzi was identified as the most prevalent species in aurochs-like breed, and the zoonotic S. hominis in Hungarian grey cattle. Concerning the sudden deaths of cattle, Sarcocystis-infection could not be demonstrated in organs showing haemorrhages, but S. cruzi cysts were present in the muscles. In one case “S. sinensis” was molecularly identified in the blood (indicating sarcocystaemia). Results of analyses for bacterial/viral pathogens were negative.ConclusionsS. cruzi appears to be the most prevalent Sarcocystis sp. in cattle in Hungary, followed by the zoonotic S. hominis. However, the rate of infection with both species was shown to differ between cattle breeds. The suspected role of Sarcocystis spp. as causative agents of the fatal cases could not be confirmed.

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“…Heydorn et al (1975) toonden aan dat het rund fungeert als tussengastheer van drie verschillende species, met respectievelijk de hond (S. cruzi), kat (S. hirsuta) en mens (S. hominis) als eindgastheer. Recente studies laten evenwel vermoeden dat er nog andere niet-geïdentificeerde species kunnen voorkomen bij het rund (Domenis et al, 2011;Vangeel et al, 2013).…”

Section: Sarcocystisunclassified

“…De letsels kunnen voorkomen bij runderen van 15 maanden tot acht jaar oud. De oorzaak is nog niet bekend, doch in verschillende gevallen kan men sarcocysten of restanten ervan centraal in het letsel vinden (Jensen et al, 1986;Gajadhar et al, 1987;Gajadhar en Marquardt, 1992;Vangeel et al, 2013). Alle species en zelfs nieuwe species kunnen in deze letsels worden waargenomen.…”

Section: Boviene Eosinofiele Myositisunclassified

See 1 more Smart Citation

Boviene sarcosporidiosis of eosinofiele myositis?

Vangeel¹,

Houf²,

Geldhof³

et al. 2014

Vlaams Diergeneeskundig Tijdschrift

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Tijdens de post-mortemkeuring en het versnijden van een rund worden grijsgroene ovale spierletsels vaak als sarcosporidiosis bestempeld, terwijl de morfologische diagnose boviene eosinofiele myositis is. Deze verwarring in terminologie kan niet alleen financiële implicaties hebben, maar leidt ook tot het invoeren van incorrecte gegevens in Europese databanken. Dit artikel vat de huidige kennis samen omtrent Sarcocystis en boviene eosinofiele myositis bij het rund, als pleidooi voor een correct gebruik van de terminologie.

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Different Sarcocystis spp. are present in bovine eosinophilic myositis

Cited by 51 publications

References 23 publications

Molecular characterisation of three regions of the nuclear ribosomal DNA unit and the mitochondrial cox1 gene of Sarcocystis fusiformis from water buffaloes (Bubalus bubalis) in Egypt

Molecular characterisation of three regions of the nuclear ribosomal DNA unit and the mitochondrial cox1 gene of Sarcocystis fusiformis from water buffaloes (Bubalus bubalis) in Egypt

Sarcocystis-infection of cattle in Hungary

Boviene sarcosporidiosis of eosinofiele myositis?

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