Different strategies for producing naturally soluble form of common cytokine receptor γ chain

Jeong, Junhui; Kim, Woo Hyun; Fernandez, Cherry P.; Kim, Suk; Kim, Yonghwan; Jang, Hyung‐Kwan; Lillehoj, Hyun S.; Woo, Hee-Jong; Min, Wongi

doi:10.1016/j.dci.2014.08.008

Cited by 2 publications

(2 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Duck splenic lymphocytes isolated from two-week-old ducks and duck embryonic fibroblast cells (DEF) obtained from ten-dayold embryos were cultured as previously described (Jeong et al, 2015;Kim et al, 2015). Cells were collected, washed twice with PBS, and cultured in DMEM (Gibco Life Technologies, USA) supplemented with 10% fetal bovine serum (Gibco Life Technologies) and penicillin-streptomycin (10,000 unit/ml) in a 41 C incubator in 5% CO 2 .…”

Section: Cell Culturementioning

confidence: 99%

“…The avian immune system provides an important model for the study of basic and applied immunology. Despite the general lack of cross-reactivity and the low level of sequence conservation between avian and mammalian cytokines (Gibson et al, 2012;Jeong et al, 2015;Stewart et al, 2013), our understanding of avian immunobiology increased extensively over the past decade. Recently, the growing literature on the critical roles of the IL-17/IL-17R axis in host immune responses includes many reports that describe the importance of the IL-17 family of cytokines in avian immunity (Hong et al, 2008;Kim et al, 2012Kim et al, , 2014Min et al, 2013;Rychlik et al, 2014;Zhao et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification and expression analysis of duck interleukin-17D in Riemerella anatipestifer infection

Diaz

Kim

Fernandez

et al. 2016

Developmental & Comparative Immunology

Self Cite

View full text Add to dashboard Cite

Interleukin (IL)-17D is a proinflammatory cytokine with currently largely unknown biological functions. Here we provide the description of the sequence, bioactivity, and mRNA expression profile of duck IL-17D homologue. A full-length duck IL-17D (duIL-17D) cDNA with a 624-bp coding region was identified from the large intestine. duIL-17D shares approximately 94.7% identity with its chicken counterpart, which is also identified in this work. duIL-17D exhibits 62.6-68.4% and 52.1-53.1% identity with mammalian and piscine homologues. Recombinant duIL-17D promoted the expression of proinflammatory cytokines such as IL-6, IL-8, and IL-1β in duck embryo fibroblast cells. Very low levels of duIL-17D transcript were observed in healthy lymphoid tissues, including bursa, thymus, and spleen, while duIL-17D expression was relatively high in the heart. The duIL-17D expression profiles were examined in mitogen-stimulated splenic lymphocytes, as well as tissues affected by Riemerella anatipestifer infection. The levels of duIL-17D were mostly upregulated in mitogen-activated splenic lymphocytes but downregulated in the liver and spleen of R. anatipestifer-infected ducks. These results provide new insights into the roles of IL-17D in host protective immune responses to Riemerella infection, which can therefore lead to further studies of its biological functions in different disease models of ducks and other avian species.

show abstract

Section: Cell Culturementioning

confidence: 99%