Differential Analysis of Active and Inactive Genes in Human Neutrophils by Chromosomal In Situ Hybridization

Izumi, Shinichi; Shin, Masashi; Takano, Kunio; Nakane, Paul K.; Koji, Takehiko

doi:10.1267/ahc.36.325

Cited by 1 publication

(1 citation statement)

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“…Since the in vivo cryotechnique has the added benefit of enhancing the immunoreactivity of certain antigens, as described above, it can also be expected to have great utility in the analyses of dynamic molecular changes occurring through various forms of stimulation. It is also conjectured that cryotechniques can reduce pretreatment steps not only in FISH, but also other intranuclear labeling methods, such as TdT-mediated dUTP-biotin nick end-labeling (TUNEL) and in situ nick translation (ISNT) [7,28].…”

Section: Significance Of Cryotechniques For Immunohistochemistrymentioning

confidence: 99%

Advanced Application of the In Vivo Cryotechnique to Immunohistochemistry for Animal Organs

Ohno

Terada

Ohno

2004

Acta Histochem. Cytochem.

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The quick-freezing method often used for physical fixation also contributed enormously to the advancement of morphology, but it could not provide enough information about the dynamic morphological changes in vivo in living animal organs. Therefore, we developed the in vivo cryotechnique in 1995, which could directly cryofix organs in vivo under anesthetized conditions without stopping blood circulation or producing effects of anoxia. We have already reported new findings about the in vivo ultrastructures of living animal organs with the cryotechnique followed by freeze-substitution or replica preparation. Recently, it has also been applied to other morphological analyses in vivo, including immunohistochemistry and FISH, for obtaining dynamically functioning structures of cells and tissues at a light microscopic level. In such experimental processes, the in vivo cryotechnique has been shown to have the added benefit of direct antigen-retrieval effects since it reduces several steps of retrieval treatments which are required in common paraffin-embedded samples prepared by the conventional fixation and dehydration. In conclusion, the in vivo cryotechnique allows us to investigate the functioning real morphology of living animals, which was technically difficult to be observed before, and perform dynamic immunohistochemistry of cells and tissues in various animal organs in vivo at ultimate time-resolution.

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Section: Significance Of Cryotechniques For Immunohistochemistrymentioning

confidence: 99%