Differential anti-proliferative effect on K562 leukemia cells of Lippia alba (Verbenaceae) essential oils produced under diverse growing, collection and extraction conditions

García, Liliana Torcoroma; Leal, Andrés Felipe; Moreno, Érika Marcela; Stashenko, Elena E.; Arteaga, H. José

doi:10.1016/j.indcrop.2016.11.057

Cited by 29 publications

(25 citation statements)

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“…The oil extraction was performed by microwave-assisted hydrodistillation (MWHD), as described elsewhere [ 25 , 29 ]. Briefly, a domestic microwave oven (Kendo, 2.45 GHz, 800 W) was modified with a side orifice through which an external Dean-Stark trap joined a round flask that contained the plant material (100 g) and water (0.5 L), inside the oven.…”

Section: Methodsmentioning

confidence: 99%

“…The presence of citral, caryophyllene and limonene has been identified in EOs isolated from two chemotypes (Citral and Carvone) of the aromatic shrub Lippia alba (Miller) N.E. Brown (Verbenaceae), that grows in the Colombian province of Santander [ 21 , 25 ]. L. alba represents the seventh species most cited in traditional Brazilian medicine [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

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Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide)

Moreno

Pinto

Stashenko

et al. 2018

BMC Complement Altern Med

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BackgroundChagas Disease caused by Trypanosoma cruzi infection, is one of the most important neglected tropical diseases (NTD), without an effective therapy for the successful parasite eradication or for the blocking of the disease’s progression, in its advanced stages. Due to their low toxicity, wide pharmacologic spectrum, and potential synergies, medicinal plants as Lippia alba, offer a promising reserve of bioactive molecules. The principal goal of this work is to characterize the inhibitory properties and cellular effects of the Citral and Carvone L. alba chemotype essential oils (EOs) and their main bioactive terpenes (and the synergies among them) on T. cruzi forms.MethodsTwelve L. alba EOs, produced under diverse environmental conditions, were extracted by microwave assisted hydrodistillation, and chemically characterized using gas chromatography coupled mass spectrometry. Trypanocidal activity and cytotoxicity were determined for each oil, and their major compounds, on epimastigotes (Epi), trypomastigotes (Tryp), amastigotes (Amas), and Vero cells. Pharmacologic interactions were defined by a matrix of combinations among the most trypanocidal terpenes (limonene, carvone; citral and caryophyllene oxide). The treated cell phenotype was assessed by fluorescent and optic microscopy, flow cytometry, and DNA electrophoresis assays.ResultsThe L. alba EOs displayed significant differences in their chemical composition and trypanocidal performance (p = 0.0001). Citral chemotype oils were more trypanocidal than Carvone EOs, with Inhibitory Concentration 50 (IC50) of 14 ± 1.5 μg/mL, 22 ± 1.4 μg/mL and 74 ± 4.4 μg/mL, on Epi, Tryp and Amas, respectively. Limonene exhibited synergistic interaction with citral, caryophyllene oxide and Benznidazole (decreasing by 17 times its IC50) and was the most effective and selective treatment. The cellular analysis suggested that these oils or their bioactive terpenes (citral, caryophyllene oxide and limonene) could be inducing T. cruzi cell death by an apoptotic-like mechanism.ConclusionsEOs extracted from L. alba Citral chemotype demonstrated significant trypanocidal activity on the three forms of T. cruzi studied, and their composition and trypanocidal performance were influenced by production parameters. Citral, caryophyllene oxide, and limonene showed a possible induction of an apoptotic-like phenotype. The best selective anti-T. cruzi activity was achieved by limonene, the effects of which were also synergic with citral, caryophyllene oxide and benznidazole.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide)

Moreno

Pinto

Stashenko

et al. 2018

BMC Complement Altern Med

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“…In addition, essential oils have significant effects on various cancer cell lines [43][44][45][46][47]. In this study, essential oil was generated from Inula graveolens L. Bornyl acetate was the chief compound (Table 1).…”

Section: Resultsmentioning

confidence: 99%

Inhibition of Various Cancer Cells Proliferation of Bornyl Acetate and Essential Oil from Inula graveolens (Linnaeus) Desf.

Karan¹,

Yildiz²,

Aydın³

et al. 2018

Rec.Nat.Prod.

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Inula species are medicinal and aromatic plants used for folk medicine extensively. In this work, hydrodistilled essential oil of Inula graveolens (Linnaeus) Desf. analyzed by GC-MS which revealed that bornyl acetate was the major product (69.15%). Camphene was the second major compound (11.11%). Antiproliferative activity of the essential oil and bornyl acetate was investigated on HeLa (human cervix carcinoma), HT29 (human colon carcinoma), A549 (human lung carcinoma), MCF7 (human breast adenocarcinoma) cancer cells and FL (human amnion cells) normal cells. The cytotoxicity was executed by a Lactate Dehydrogenase (LDH) Cytotoxicity Detection Kit. Essential oil and bornyl acetate displayed the outstanding activities on HeLa (IC 50 , 64.1, 72.0 µg/mL), HT29 (IC 50 , 24.6, 60.5 µg/mL), A549 (IC 50 , 28.3,44.1 µg/mL), MCF-7 (IC 50 , 66.5, 85.6 µg/mL), and FL (IC 50 , 42.1, 50.6 µg/mL) cell lines respectively.

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“…Essential oils from the leaves of L. alba have been categorized into different chemotypes, depending on their major constituents, such as linalool, citral, and carvone (Pandeló et al, 2012). Several biological properties of this plant, such as cytotoxicity, antioxidant, antibiofilm, anesthetic, antitumor, antibacterial, antifungal, antiinflammatory, antispasmodic activities and anxiolytic-like effects differ according to essential oil chemotype (Glamočlija et al, 2011;Trevisan et al, 2016;Tofiño-Rivera et al, 2016;Pandey et al, 2016, García et al, 2017.…”

Section: Introductionmentioning

confidence: 99%

Essential oil composition, antifungal activity and leaf anatomy of Lippia alba (Verbenaceae) from Brazilian Chaco

Arruda¹,

Victório²,

Boaretto³

et al. 2019

J. Med. Plants Res.

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This study aims to determine the essential oil chemical composition of Lippia alba (Mill.) N.E.Br. ex Britton & P. Wilson collected in the Brazilian Chaco where plants grow in conditions of high temperatures in the summer, periodic flood, low temperatures and air humidity in the winter. We also evaluate the oil antifungal activity against the animal and plant pathogenic fungi Aspergillus flavus, A. fumigatus, A. niger, A. terreus, Fusarium sp., Penicillium funiculosum and Sclerotinia sclerotiorum. Leaf essential oils were extracted by Clevenger hydrodistillation and characterized by GC-MS. The major essential oil components were linalool (38.26%), trans-ocimenone (6.57%) and caryophyllene oxide (6.48%). At first time L. alba from Brazilian Chaco was identified as a chemotype producing linalool. The essential oils showed antifungal activity, mainly against S. slerotiorum, a fungi species related with diseases in soybean plants, with 100% of growth inhibition. These results suggest the potential alternative of this species to synthetic fungicides and confirm its popular uses as an important medicinal plant in South America.

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Differential anti-proliferative effect on K562 leukemia cells of Lippia alba (Verbenaceae) essential oils produced under diverse growing, collection and extraction conditions

Cited by 29 publications

References 24 publications

Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide)

Induction of programmed cell death in Trypanosoma cruzi by Lippia alba essential oils and their major and synergistic terpenes (citral, limonene and caryophyllene oxide)

Inhibition of Various Cancer Cells Proliferation of Bornyl Acetate and Essential Oil from Inula graveolens (Linnaeus) Desf.

Essential oil composition, antifungal activity and leaf anatomy of Lippia alba (Verbenaceae) from Brazilian Chaco

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