Differential changes in cerebellar transmitter content and expression of calcium binding proteins and transcription factors in mouse administered with amphetamine

Yin, Hsiang‐Shu; Lai, Chuan-Ching; Tien, Ta–Chang; Han, Shan-Kuo; Pu, Xiang-Lin

doi:10.1016/j.neuint.2010.06.007

Cited by 8 publications

(4 citation statements)

References 48 publications

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“…Previous work has identified the pattern of cFos expression in the rat cerebellum after a repeated treatment with cocaine (Klitenick, Tham & Fibiger ) or amphetamine (Yin et al . ). Both psychostimulant drugs produced an increase in Fos+ immunoreactivity at the granule cell layer of the vermis, although cFos+ immunostaining in Purkinje cells was sparse.…”

Section: Discussionmentioning

confidence: 97%

Involving the cerebellum in cocaine-induced memory: pattern of cFos expression in mice trained to acquire conditioned preference for cocaine

et al. 2013

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Versión / Versió:Preprint Conflict of Interest (COI) StatementThe authors of the present manuscript declare no conflict of interest. AbstractDue to its capital role in drug seeking, consumption and addictive behaviour there is a growing interest in identifying the neural circuits and molecular mechanisms underlying the formation, maintenance and retrieval of drug related memories. Human studies focused on neuronal systems that store and control drug-conditioned memories have found cerebellar activations during the retrieval of drug-associated cues memory. However, at the preclinical level, almost no attention has been paid to a possible role of the cerebellum in drug-related memories. In the present study, we ought to fill this gap by aiming to investigate the pattern of neuronal activation (as revealed by cFos expression) in different regions of the prefrontal cortex and cerebellum of mice trained to develop conditioned preference for an olfactory stimulus (CS+) paired with cocaine.Our results indicate that CS+ preference was directly associated with cFos expression in cells at the apical region of the granule cell layer of the cerebellar vermis, this relationship being more prominent in some specific lobules. Conversely, cFos+ immunostaining in other cerebellar regions seems unrelated to CS+ preference but to other aspects of the conditioning procedure. At the prefrontal cortex, cFos expression seemed to be related to cocaine administration rather than to its ability to establish conditioned preference. The present results suggest that as it has been observed in some clinical studies, the cerebellum might be an important and largely overlooked part of the neural circuits involved in generating, maintaining and/or retrieving drug memories.

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Section: Discussionmentioning

confidence: 97%

Involving the cerebellum in cocaine-induced memory: pattern of cFos expression in mice trained to acquire conditioned preference for cocaine

et al. 2013

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“…Protein concentrations of the samples were quantified using the bicinchoninic acid (BCA) assay. The supernatants were further processed and subjected to the reverse‐phase high‐performance liquid chromatography (HPLC) (Waters 626 LC System (Waters Corporation, Milford, MA, USA) containing a 200 × 4.6 mm, C18, 5‐mm particle size column and a 464 pulsed electrochemical detection device) according to previous protocols (Yin et al , 2010). Briefly, the mobile phase for detecting l ‐dopa, DA and its metabolites consisted of 1.5 m acetonitrile, 0.27 m m ethylenediaminetetraacetic acid (EDTA), 0.03 m triethylamine, 10.3 m m heptanesulfonic acid and 35 m m phosphoric acid, pH 2.4.…”

Section: Methodsmentioning

confidence: 99%

Akt1 deficiency modulates reward learning and reward prediction error in mice

Chen¹,

Chen²,

Hsu

et al. 2012

Genes Brain and Behavior

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In contemporary reinforcement learning models, reward prediction error (RPE), the difference between the expected and actual reward, is thought to guide action value learning through the firing activity of dopaminergic neurons. Given the importance of dopamine in reward learning and the involvement of Akt1 in dopaminedependent behaviors, the aim of this study was to investigate whether Akt1 deficiency modulates reward learning and the magnitude of RPE using Akt1 mutant mice as a model. In comparison to wild-type littermate controls, the expression of Akt1 proteins in mouse brains occurred in a gene-dosage-dependent manner and Akt1 heterozygous (HET) mice exhibited impaired striatal Akt1 activity under methamphetamine challenge. No genotypic difference was found in the basal levels of dopamine and its metabolites. In a series of reward-related learning tasks, HET mice displayed a relatively efficient method of updating reward information from the environment during the acquisition phase of the two natural reward tasks and in the reverse section of the dynamic foraging T-maze but not in methamphetamine-induced or aversive-related reward learning. The implementation of a standard reinforcement learning model and the Bayesian hierarchical parameter estimation show that HET mice have higher RPE magnitudes and that their action values are updated more rapidly among all three test sections in T-maze. These results indicate that Akt1 deficiency modulates natural reward learning and RPE. This study showed a promising avenue for investigating RPE in mutant mice and provided evidence for the potential link from genetic deficiency, to neurobiological abnormalities, to impairment in higher-order cognitive functioning.

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“…Previously, a few reports described an enhancement in this expression in the granular cell layer and in Purkinje neurons after repeated injections of cocaine or amphetamine (Clark et al, 1992;Yin et al, 2010;Vazquez-Sanroman et al, 2015a). Nevertheless, one of the contributions of the present research is to demonstrate that the increased activity might represent a cerebellar hallmark of cocaine-induced preference conditioning.…”

Section: Discussionmentioning

confidence: 53%

“…Previous work has identified the pattern of cFos expression in the rat cerebellum after a repeated treatment with cocaine or amphetamine (Yin et al 2010). Both psychostimulant drugs produced an increase in cFos+ immunoreactivity at the granule cell layer of the vermis, although cFos+ immunostaining in Purkinje cells was sparse.…”

mentioning

confidence: 99%

Involvement of the cerebellum in cocaine-induced memory

Gas¹

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PREFACEBecause of its role in drug-seeking, consumption and addictive behavior, there is a growing interest in identifying the neural circuits and molecular mechanisms underlying the formation, maintenance and retrieval of drug-induced memories. However, very few studies have focused on brain areas beyond the corticostriatal-limbic circuitry. Despite the growing evidence confirming the involvement of the cerebellum in drug-induced alterations, this structure has been traditionally disregarded in the addiction field Miquel et al, 2016).The general aim of the present research is to address wether the cerebellum is part of the neuronal systems that sustains the plasticity mechanisms underlying drug-induced conditioned memories. We have focused our research in an attempt to clarify if the cerebellum is involved in the acquisition and storage of drug memories. Although previous scattered reports described the involvement of the cerebellum in drug-related memories, this is the first attempt to address a detailed functional analysis about the issue.The present doctoral thesis contains three different chapters. The first two include two investigations that have been already published (Carbo-gas et al., 2014ab), and the third is one currently under revision. In the first chapter, Involving the cerebellum in cocaineinduced memory: pattern of cFos expression in mice trained to acquire conditioned preference for cocaine, we explored the pattern of neuronal activation as revealed by cFos immunoreactivity of mice trained to develop conditioned preference for an olfactory stimulus paired with cocaine. In the second one, entitled Cerebellar hallmarks of conditioned preference for cocaine, we used the same behavioral task of the first study in order to further extend the description of cFos expression patterns in cerebellar circuitry, including now the major inputs and one of the output nuclei of the cerebellum. 9In the last chapter, Cerebellar perineuronal nets in cocaine-induced Pavlovian memory:site does matter, we accomplished a broad analysis of perineuronal nets (PNNs) expression of cerebellar vermis. First, we analysed an outbred mouse strain trained to acquire preference for olfactory stimuli associated with cocaine. Second, α6Cre-Cacna1a mice were used to test if a reduction in the glutamate release of parallel fibres to Purkinje dendrites would alter the acquisition of cocainepreference conditioning and the expression of PNNs in the cerebellum.After the presentation of the chapters, there is a section where summarised findings, strengths and pitfalls as well as future directions are provided. References can be also found at the end of the present document.Finally, we conclude this thesis with an appendix. This document is formed by research not finished yet, but that may be of great relevance to understand the role of the cerebellum in addictive disorders. In that appendix, we explored the effect of a focal lesion in the dorsal cerebellar cortex (Lobule VIII) on the acquisition of food selfadministration. This researc...

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Differential changes in cerebellar transmitter content and expression of calcium binding proteins and transcription factors in mouse administered with amphetamine

Cited by 8 publications

References 48 publications

Involving the cerebellum in cocaine-induced memory: pattern of cFos expression in mice trained to acquire conditioned preference for cocaine

Involving the cerebellum in cocaine-induced memory: pattern of cFos expression in mice trained to acquire conditioned preference for cocaine

Akt1 deficiency modulates reward learning and reward prediction error in mice

Involvement of the cerebellum in cocaine-induced memory

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