Differential chromosomal markers between sympatric karyomorphs of the fish Hoplias malabaricus (Bloch, 1794) (Characiformes: Erythrinidae)

Rosa, Renata da; Rubert, Marceléia; Giuliano-Caetano, Lúcia; Martins‐Santos, Isabel Cristina

doi:10.3897/compcytogen.v4i2.48

Cited by 4 publications

(3 citation statements)

References 35 publications

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“…Within the Parana River basin, the most usual cases of sympatry are among karyomorphs A/C, commonly found in the lower Parana River, and A/D, usually found in the upper Parana River (Bertollo et al, 2000;Rosa et al, 2009Rosa et al, , 2010. Moreover, in the upper Parana River, one A/C/D sympatry case has also been reported (Pazza & Júlio, 2003).…”

Section: Discussionmentioning

confidence: 99%

“…To date, seven easily distinguishable karyomorphs (A-G) were identified based on chromosome number, chromosome morphology and the presence or absence of sex chromosomes (Bertollo et al, 2000). Although many cases of sympatric karyomorphs have been described, there is no evidence of hybridization between them until now (Scavone et al, 1994;Lopes et al, 1998;Bertollo et al, 2000;Pazza & Júlio, 2003;Rosa et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Genetic differentiation among distinct karyomorphs of the wolf fish Hoplias malabaricus species complex (Characiformes, Erythrinidae) and report of unusual hybridization with natural triploidy

Utsunomia

Alves

Paiva

et al. 2014

Journal of Fish Biology

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In this study, genetic differentiation between karyomorphs A (2n = 42) and D (2n = 39/40) of the wolf fish Hoplias malabaricus, which is comprised of several cryptic species that present a wide variety of diploid chromosome numbers and sex chromosome systems, resulting in the identification of seven distinct karyomorphs (A-G), was investigated using a combination of molecular and cytogenetic tools. Deep sequence divergences for both karyomorphs were observed and indicate a long period of reproductive isolation between karyomorphs A and D. Additionally, one individual with 61 chromosomes was identified, which, as far as is known, is the first case of natural triploidy resulting from the hybridization between these highly differentiated karyomorphs of H. malabaricus. Molecular and cytogenetic analyses revealed that this allotriploid specimen carries two sets of maternal chromosomes from karyomorph D (2n = 40) and one set of chromosomes from karyomorph A (n = 21). Moreover, ribosomal sites and active nucleolus organizer regions from both parental contributors were found in the triploid hybrid. Considering the significant genetic distances between karyomorphs A and D, one of the primary reasons for the lack of recurrent reports of hybridization in the H. malabaricus species complex may be due to post-zygotic barriers, such as hybrid sterility or unviability.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic differentiation among distinct karyomorphs of the wolf fish Hoplias malabaricus species complex (Characiformes, Erythrinidae) and report of unusual hybridization with natural triploidy

Utsunomia

Alves

Paiva

et al. 2014

Journal of Fish Biology

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“…Some karyomorphs as A, C and F have a wide geographic distribution throughout South America, whereas others are either endemic or restricted to specific drainages in Brazil (Bertollo et al 2000, Da Rosa et al 2014). In some cases, two and even more karyomorphs have been found coexisting in sympatric conditions without detection of hybrid forms (Lopes and Fenocchio 1994, Scavonne et al 1994, Lopes et al 1998, Lemos et al 2002, Pazza and Júlio 2003, Born and Bertollo 2006, Da Rosa et al 2009a, 2009b, 2010). …”

Section: Introductionmentioning

confidence: 99%

Cytogenetic characterization of Hoplias malabaricus (Bloch, 1794) from the Ctalamochita River (Córdoba, Argentina): first evidence for southernmost populations of this species complex and comments on its biogeography

Grassi¹,

Swarça²,

Dergam³

et al. 2017

CCG

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Hoplias malabaricus (Bloch, 1794), a predatory freshwater fish with a wide distribution throughout South America, represents a species complex with seven well characterized karyomorphs at the cytogenetic level. Although this species has been extensively studied in several Brazilian basins, data are still scarce for hydrographic systems from other South American countries. This study aims to characterize cytogenetically the Hoplias malabaricus populations from the Argentinean Central Region, close to the southernmost distribution of this species complex. A total of 32 specimens from the Ctalamochita River, a tributary of Lower Paraná Basin located in the province of Córdoba, were analyzed using cytogenetic techniques (Giemsa staining, C- and Ag-NOR banding and fluorescent in situ hybridization with 18S rDNA). All the specimens showed diploid number 2n=42, chromosomic formula 22m + 20sm and absence of sexual chromosomes. Thus, the analyzed populations belong to the karyomorph named A. These populations showed a remarkable degree of divergence in their cytogenetic traits such as karyotypic formula, C-banding, NORs and 18S rDNA patterns for Hoplias malabaricus from other populations bearing the same karyomorph in the Middle and Upper Paraná Basin. These findings are consistent with molecular data from a recent study (where specimens collected in the present work were included), which indicate a closer phylogenetic relationship of Hoplias malabaricus populations from the Ctalamochita River with those from the Uruguay basin and the coastal regions of South Brazil than with populations from the Middle and Upper Paraná Basin. Overall, these pieces of evidence highlight the distinctive features of Hoplias malabaricus from the Ctalamochita River, and also reveal a complex history of dispersion of these populations. The present work is the first to provide cytogenetic information and include some phylogeographic aspects of Hoplias malabaricus populations living in close proximity to the southernmost extreme of its distribution area. Therefore, this study expands significantly upon the previously known geographical coverage for karyomorph A and contributes to a better understanding of the karyotypic diversification within this species complex.

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New Insights into the Biogeographic and Karyotypic Evolution ofHoplias Malabaricus

RosaRenata

Vicari²,

Lúcia³

et al. 2014

Zebrafish

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Hoplias malabaricus is a species widely distributed throughout Brazil. Cytogenetic studies indicate the occurrence of extensive chromosomal rearrangements in population differentiation and speciation of the group that demonstrated an independent origin of sex chromosome systems. Seven karyomorphs were characterized for the species and are located in specific river basins, while others are distributed throughout several different basins. However, there are few studies linking the geographical distribution of H. malabaricus karyomorphs to the Brazilian hydrographic basins. This article provides new chromosomal information on five populations of H. malabaricus collected in a South Atlantic basin. The samples were analyzed by conventional and molecular cytogenetic techniques. Two karyomorphs, A (2n=42 m/sm) and C (2n=40 m/sm), were detected, and remarkable differences in the distribution of heterochromatin and GC-rich blocks were observed in the karyomorphs. A review of existing data is presented here, where we observe that dispersion is associated with the genesis of the South and Central America river basins. Coastal drainages represent an ancestral biogeographical component for many groups of fish, representing older basins, such as the basins of the Eastern Atlantic and San Francisco river, suggesting that existing karyomorphs found in these basins may represent a basal karyotype (karyomorph F) within H. malabaricus. The current cytogenetic data, including this article, for different karyomorphs of H. malabaricus in conjunction with the geological history of the continent allow us to determine that the ancestral group is most likely karyomorph F.

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Differential chromosomal markers between sympatric karyomorphs of the fish Hoplias malabaricus (Bloch, 1794) (Characiformes: Erythrinidae)

Cited by 4 publications

References 35 publications

Genetic differentiation among distinct karyomorphs of the wolf fish Hoplias malabaricus species complex (Characiformes, Erythrinidae) and report of unusual hybridization with natural triploidy

Genetic differentiation among distinct karyomorphs of the wolf fish Hoplias malabaricus species complex (Characiformes, Erythrinidae) and report of unusual hybridization with natural triploidy

Cytogenetic characterization of Hoplias malabaricus (Bloch, 1794) from the Ctalamochita River (Córdoba, Argentina): first evidence for southernmost populations of this species complex and comments on its biogeography

New Insights into the Biogeographic and Karyotypic Evolution ofHoplias Malabaricus

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