Differential circRNA expression profiles during the BMP2-induced osteogenic differentiation of MC3T3-E1 cells

Qian, Dongyang; Gao, Yan; Bai, Bo; Chen, Yi; Zhang, Shujiang; Yao, Yongchang; Xia, Hong

doi:10.1016/j.biopha.2017.03.051

Cited by 80 publications

(63 citation statements)

References 29 publications

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“…In the past, some circRNAs, like circ19142 and circ5846, are reported to be involved in BMP2-reduced osteoblast differentiation, through a circ19142/circ5846-targeted miRNA-mRNA axis. 6 However, the role of circRNAs in this process, for instance, hsa-circ-0076694 (circRUNX2), remained to be researched. In this research, we focused on how circRUNX2 influenced osteoporosis and revealed the mechanism of osteogenic differentiation related to cir-cRUNX2.…”

Section: Circrunx2 and Mir-203 Affected Osteogenic Differentiation mentioning

confidence: 99%

“…For example, Liu et al found 2:27713879|27755789 and 2:240822115|240867796 participated in regulation network in osteogenic differentiation . Also, they were able to affect the expression of some essential carcinogenic mRNAs by targeting miRNAs and changing the miRNAs expression which would subsequently change the miRNAs level …”

Section: Introductionmentioning

confidence: 99%

“…5 Also, they were able to affect the expression of some essential carcinogenic mRNAs by targeting miRNAs and changing the miRNAs expression which would subsequently change the miRNAs level. 6 It was predicted that circRNAs would combine with miRNA and compete for the binding sites on the miRNA with mRNAs. 7 Cir-cRNAs expression would alter the mRNA expression by targeting miRNAs that were binding to the mRNAs chosen.…”

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confidence: 99%

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CircRUNX2 through has‐miR‐203 regulates RUNX2 to prevent osteoporosis

Yin

Wang

et al. 2018

J Cellular Molecular Medi

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ObjectiveWe aimed to discover the molecular mechanism of hsa_circ_0076694 (circRUNX2) on osteogenic differentiation. We also explored the interaction between circRUNX2, miR‐203 and RUNX2.MethodsClinical samples obtained from femoral neck fracture patients’ bone tissues were used to collect circRUNX2, miR‐203, and RUNX2 expression data, while their expression changes were observed in human bone mesenchymal stem cells (hBMSCs) during osteogenic differentiation. QRT‐PCR and Western blot were used to analyse levels of RNAs and proteins. Biotin pull down, RIP, RNA FISH, and Dual‐Luciferase Reporter assays demonstrated the relationship between circRUNX2, miR‐203, and RUNX2. ALP and ARS staining were used to measure the degree of osteogenic differentiation under the control of circRUNX2, miR‐203.ResultsCircRUNX2 were down‐regulated in osteoporotic patients’ bone tissues. CircRUNX2 could inhibit miR‐203 expression by sponging miR‐203. MiR‐203 inhibited osteogenic differentiation by targeting the 3′‐UTR of RUNX2 and down‐regulate RUNX2 expression. Overexpression of circRUNX2 promoted the expression of osteogenic differentiation‐related proteins such as RUNX2, OCN, OPN, BSP, and prevented osteoporosis.ConclusioncircRUNX2 could sponge miR‐203 and enhance RUNX2 expression, thus circRUNX2 prevents osteoporosis and may provide a novel therapeutic strategy for it.

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Section: Circrunx2 and Mir-203 Affected Osteogenic Differentiation mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 99%

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CircRUNX2 through has‐miR‐203 regulates RUNX2 to prevent osteoporosis

Yin

Wang

et al. 2018

J Cellular Molecular Medi

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“…circrna 19142 (circ19142) and circrna 5846 (circ5846) act as sponges for mir-7067-5p; this is a network that is confirmed to function in osteoblast differentiation through a circ19142/circ5846-targeted mirna-mrna axis (61). dou et al (62) reported that 19 circrnas were upregulated and five circRNAs were downregulated during different stages of mouse osteoclast formation.…”

Section: Circrnas In Osteoblasts and Osteoclastsmentioning

confidence: 94%

The potential roles of circular RNAs in osteonecrosis of the femoral head (Review)

Zhao

Wang

et al. 2019

Mol Med Report

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circular rnas (circrnas) are categorized as non-coding rnas that, unlike widely known canonical linear rnas, form a covalently closed continuous loop without 5' or 3' polarities, which enables them to resist digestion by rna exonucleases. although the functions of circrnas remain largely unknown, accumulated evidence has demonstrated that circrnas can act as microrna sponges, which allows them to regulate numerous biological processes and disease mechanisms, including apoptosis, angiogenesis, invasion, metastasis and stem cell differentiation. although research into circRNAs is in its infancy, studies have identified critical roles for circrnas in the initiation and progression of disease. The present study delineated the characteristics and functions of circrnas, and focused on the potential relationship between circrnas and osteonecrosis of the femoral head (onFH). circrnas represent a novel avenue for studying the mechanisms underlying onFH as well as possible treatments.

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“…The alkaline phosphatase (ALP) activity of the MC3T3-E1 cells was used to evaluate the osteoblast differentiation [24]. The compound p-nitrophenyl phosphate (PNPP) produces a yellow product and has maximum absorbance at 405 nm, as measured with an ELISA reader (Sunrise-basic, Tecan, Männedorf, Zürich, Switzerland).…”

Section: Cell Test In Vitromentioning

confidence: 99%

Nano/Micro Hierarchical Bioceramic Coatings for Bone Implant Surface Treatments

et al. 2020

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Bone implants with surface modifications that promote the physiological activities of osteoblasts are the first step for osseointegration in bone repair. Hydroxyapatite is the main inorganic component in mammal bones and teeth, and nanoscaled hydroxyapatite promotes the adhesion of osteoblastic cells. In this study, we created a nano/micro hierarchical structure using micro-arc oxidation coatings and hydrothermal treatments at 150 °C, 175 °C, and 200 °C for 2, 6, 12, and 24 h. After undergoing hydrothermal treatment for 24 h, CaTiO3 began forming regular-shaped crystals at the surface at 175 °C. In order to decrease the CaTiO3 formations and increase the apatite fabrication, a shorter time of hydrothermal treatment was required at 175 °C. There was still surface damage on samples treated for 6 h at 175 °C; however, the nano/micro hierarchical structures were formed in 2 h at 175 °C. The normalized alkaline phosphatase (ALP) activities of the MC3T3-E1 cells with micro-arc oxidation (MAO) coatings and nano/micro hierarchical bioceramics coatings were 4.51 ± 0.26 and 7.36 ± 0.51 μmol p-NP/mg protein (*** P value of <0.001), respectively. The MC3T3-E1 cells with coatings showed highly statistically significant results in terms of the ALP activity. This proposed nano/micro hierarchical structure promoted cell proliferation and osteogenic differentiation of the osteoblast MC3T3-E1 cells. This study realized a promising nano system for osseointegration via bone implant surface treatments, which can promote the physiological activities of osteoblasts.

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Differential circRNA expression profiles during the BMP2-induced osteogenic differentiation of MC3T3-E1 cells

Cited by 80 publications

References 29 publications

CircRUNX2 through has‐miR‐203 regulates RUNX2 to prevent osteoporosis

CircRUNX2 through has‐miR‐203 regulates RUNX2 to prevent osteoporosis

The potential roles of circular RNAs in osteonecrosis of the femoral head (Review)

Nano/Micro Hierarchical Bioceramic Coatings for Bone Implant Surface Treatments

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