Differential Display Analysis of the Early Compatible Interaction Between Soybean and the Soybean Cyst Nematode

Abstract

The marked cellular changes during feeding site formation of the soybean cyst nematode (Heterodera glycines) indicate major changes in soybean gene expression. We used differential display of mRNA to detect host gene expression changes during the early compatible interaction between soybean and H. glycines. Fifteen cDNA clones corresponding to mRNAs with different abundances in H. glycines-infected versus uninfected roots were identified. Differential display results indicated that abundances of five mRNAs increased in infected roots, whereas abundances of 10 mRNAs decreased. Transcripts for nine of these 15 cDNAs could be detected on RNA blots, and their hybridization signals confirmed the differential display results for eight of these nine cDNAs. Sequence analyses identified five cDNAs with decreased mRNA levels in infected roots as corresponding to two putative aldolase genes, a transcription-factor TFIIA homologue, the soybean small GTP-binding protein gene sra1, and the soybean auxin down-regulated gene ADR12. RNA blot analyses of other auxin down-regulated genes revealed a decrease in their mRNA abundances in H. glycines-infected roots as well.

Transcriptomic Analysis of Nematode Infestation

“…As molecular biology techniques improved, PCR enrichment strategies were employed to identify transcripts directly from cDNA libraries in response to nematode infections. Frequently, whole root systems were used as a starting material for RNA isolation (Hermsmeier et al 1998 ;Mahalingam et al 1999 ;Vaghchhipawala et al 2001) . Furthermore, chances of identifying genes that were differentially regulated by nematode infections were improved by collecting tissues enriched for nematode feeding sites using approaches of preselecting infested tissues (for example, giant-cell or syncytial containing tissues) (Hermsmeier et al 2000 ;Vercauteren et al 2001) , by manually dissecting feeding sites or by microaspiration (Wieczorek et al 2006) .…”

“…A differential display analysis comparing transcript abundance in H. glycines infested and noninfested soybean roots identified 15 gene transcripts that were differentially regulated one day after inoculation (Hermsmeier et al 1998) . To identify nematode-regulated transcripts, Hermsmeier and colleagues used primers that were specially designed to amplify random cDNAs from infested and control libraries.…”

Comparative profiling of the transcriptional response to soybean cyst nematode infection of soybean roots by deep sequencing

“…The magnitude of the changes in soybean during SCN-infection suggests that many genes are involved. Several genes related to SCN-infection have been identified in soybean, including those encoding polygalacturonases [3], an ethylene-responsive element-binding protein [4], a phosphoribosylformyl-glycinamidine synthase [5], an extension [6], a cyclin [7], a GTP-binding protein [8], etc. In recent years, several studies profiling global soybean responses to SCN infection using differential display and microarray approaches have increased our understanding of changes in gene expression during SCN-infection [9][10][11][12].…”

Nematode Resistance in Soybean

“…In an earlier investigation, Hermsmeier et al (1998) used differential display of mRNA to detect host gene expression changes during the early compatible interaction between soybean and SCN. The authors identified 15 genes with different expressions in SCN-infected versus uninfected roots.…”

“…The authors identified 15 genes with different expressions in SCN-infected versus uninfected roots. Of those, the ADR12 gene was identified to be involved in soybean auxin downregulation, suggesting that the auxin pathway may be involved in soybean-nematode interaction (Hermsmeier et al 1998).…”