Differential effects of the persistent DDT metabolite methylsulfonyl-DDE in nonstimulated and LH-stimulated neonatal porcine Leydig cells

Castellanos, Cesilie Granum; Sørvik, Irene Beate; Tanum, Marte Bruu; Verhaegen, Steven; Brandt, Ingvar; Ropstad, Erik

doi:10.1016/j.taap.2012.12.022

Cited by 12 publications

(4 citation statements)

References 52 publications

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“…Evaluation of fshb and lhb transcription can be used to determine whether environmental chemicals cause disruption of the HPG axis (Castellanos et al, 2013). Except for mRNA expression of fshb in the brains of females, RNA expression of fshb and lhb was up-regulated in rare minnows in the 20 and 200 mg/L treatments.…”

Section: Discussionmentioning

confidence: 99%

2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus)

Chen

Cao

Yuan

et al. 2016

Environmental Pollution

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a b s t r a c t 2,4-Dichloro-6-nitrophenol (DCNP) is an environmental transformation product of 2,4-dichlorophenol that has been identified as widespread in effluent wastewater, but little is known about its toxicity because this compound is not regulated. Therefore, to investigate the endocrine disruption potency of DCNP in Chinese rare minnows (Gobiocypris rarus), adult and juvenile fish were exposed to various concentrations of DCNP (2, 20, and 200 mg/L) for 28 d. After 28 d exposure, the plasma vitellogenin (VTG) levels were reduced in females while increased in males and juvenile fish considerably, as compared with the control. These results suggested that DCNP affects the HPG-axis in a sex-dependent way. Testosterone (T) levels in the plasma were significantly lower in adult and juvenile fish and were accompanied by an increased estradiol (E2)/T ratio. Histopathological observation revealed hypertrophy of the hepatocytes and nuclear pyknosis in the liver, the inhibition of spermatogenesis in the testes, and the degeneration of oocytes in the ovaries after DCNP exposure. The expression pattern of selected genes indicated that the nuclear receptor, steroidogenesis and gonadotropin regulation pathways were perturbed after DCNP exposure. Above all, our results demonstrated that DCNP clearly had anti-androgenic activity in both adult and juvenile fish and can therefore be considered as an endocrine-disrupting chemical.

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Section: Discussionmentioning

confidence: 99%

2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus)

Chen

Cao

Yuan

et al. 2016

Environmental Pollution

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“…Using IHC, we found that NMBR was expressed in Leydig cells; therefore, we believed that NMB in the Leydig cells had an effect. In 3-to 5-week-old pig testes, the percentage and volume of Leydig cells are high and the Leydig cells in primary culture retain steroidogenic capacity; additionally, between birth and 1 month of age, the number of Leydig cells per testis showed a significant increase (Franca et al 2000); therefore, researchers usually study steroidogenesis in a primary culture of immature porcine Leydig cells as models, which study the steroidogenesis of adult porcine Leydig cells and the development of Leydig cells (Bernier et al 1983, Lejeune et al 1998, Mauduit et al 2001, Fombonne et al 2003, Nakajima et al 2005, Honda et al 2008, Lervik et al 2011, Castellanos et al 2013. In this study, we employed immature porcine Leydig cells to study the effects of NMB on boar reproduction and treated the primary culture with different doses (0.01-1000 nM) of NMB (Saito et al 2013).…”

Section: Discussionmentioning

confidence: 99%

Effects of neuromedin B on steroidogenesis, cell proliferation and apoptosis in porcine Leydig cells

Zhang

et al. 2018

Journal of Molecular Endocrinology

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Neuromedin B (NMB), a mammalian bombesin-related peptide, has numerous physiological functions, including regulating hormone secretions, cell growth, and reproduction, by binding to its receptor (NMBR). In this study, we investigated the effects of NMB on testosterone secretion, steroidogenesis, cell proliferation, and apoptosis in cultured primary porcine Leydig cells. NMBR was mainly expressed in the Leydig cells of porcine testes, and a specific dose of NMB significantly promoted the secretion of testosterone in the primary Leydig cells; moreover, NMB increased the expression of mRNA and/or proteins of NMBR and steroidogenic mediators (steroidogenic acute regulatory (STAR), CYP11A1, and HSD3B1) in the Leydig cells. In addition, specific doses of NMB promoted the proliferation of Leydig cells and increased the expression of proliferating cell nuclear antigen and Cyclin B1 proteins, while suppressing Leydig cell apoptosis and decreasing BAX and Caspase-3 protein expression. These results suggest that the NMB/NMBR system might play an important role in regulating boar reproductive function by modulating steroidogenesis and/or cell growth in porcine Leydig cells.

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“…Pesticides, insecticide Tapiero et al [124] Tesier and Matsumura [125] Castellanos et al [126] mediates reactions on distant tissues and cells. Therefore, research that focuses only on isolated components of endocrine system or target tissues may provide incomplete information.…”

Section: Endocrine Disruptorsmentioning

confidence: 99%

Endocrine Disruptors and Reproductive Health in Males

Jambor¹,

Greifova²,

Bistakova³

et al. 2018

Endocrine Disruptors

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Nowadays, endocrine-disrupting chemicals are considered to be one of the main causes of the ever-increasing occurrence of problems with male fertility. These compounds of natural or anthropogenic origin are omnipresent in the environment and organisms are exposed to them practically nonstop through the air, water, food, and occupationally. Endocrine disruptors have the ability to mimic effects of reproductive hormones and demonstrably can interfere with the endocrine system leading to reproductive disorders at different levels, and considering male reproductive functions, most of the impacts are performed by the breakdown of estrogen-or androgen-mediated processes. A significant body of evidence based upon laboratory or wildlife animal experiments and metaanalysis of semen studies in men indicates that exposure to endocrine disrupting compounds is associated with male reproductive malfunctions, including impairment of spermatogenesis followed by reduced semen quality parameters (sperm concentration, motility, and morphology). Alkylphenols, bisphenol, and phthalates are substantial components of many products with which people come into contact daily. This brief review will emphasize on the possible effects of alkylphenols, bisphenol, and phthalates on the male reproductive system, and current research efforts related to these substances mainly in the context of two main processes taking place in testicular tissues-steroidogenesis and spermatogenesis.

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Differential effects of the persistent DDT metabolite methylsulfonyl-DDE in nonstimulated and LH-stimulated neonatal porcine Leydig cells

Cited by 12 publications

References 52 publications

2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus)

2, 4-Dichloro-6-nitrophenol, a photonitration product of 2, 4-dichlorophenol, caused anti-androgenic potency in Chinese rare minnows (Gobiocypris rarus)

Effects of neuromedin B on steroidogenesis, cell proliferation and apoptosis in porcine Leydig cells

Endocrine Disruptors and Reproductive Health in Males

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