Differential Expressed Protein in Developing Stages of Nepenthes gracilis Korth. Pitcher

Pinthong, Krit; Chaveerach, Arunrat; Tanee, Tawatchai; Sudmoon, Runglawan; Mokkamul, Piya

doi:10.3923/pjbs.2009.526.529

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…This study showed that pitcher formation might be correlated with the gene expressed in the leaf. Thus, this study suggests that pitcher formation is part of leaf development [28].…”

Section: Resultsmentioning

confidence: 53%

Pitcher formation of Nepenthes ampullaria and Nepenthes rafflesiana on modified in vitro media

Isnaini,

Novitasari

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Nepenthes, known as pitcher plants, is one of the unique ornamental plants that are highly sought after for their unique shape and color of the pitchers, a modification of the leaves. The pitcher was reportedly formed under nutrient-poor conditions at its growth site. This study aims to investigate the effects of modification of the culture media on the formation of in vitro pitchers of Nepenthes ampullaria and N. rafflesiana. The experimental design in this study used a completely randomized design with two factors (the species of Nepenthes and the media). Nepenthes ampullaria and N. rafflesiana plantlets with a stem height of about 1-2 cm were planted in medium containing half strength of Murashige and Skoog (1/2 MS), in medium containing sugar, agar, and distilled water (SAW), in medium containing agar and distilled water (AW) and medium containing sterile distilled water (W). All media were adjusted to a pH of about 5.7. The filter paper was used as a buffer in a liquid medium to support the plantlets. Each treatment was replicate three times, with each replicate containing ten bottles of culture, each containing one plantlet. Research results were observed for 12 weeks after planting and showed that all cultures could grow well in each medium. The highest average number of pitchers was recorded for the N. ampullaria culture planted in AW medium, with 22 pitchers/plant, followed by the culture in W medium, with 20 pitchers/plant. For N. rafflesiana, on the other hand, the highest number of pitchers was found in W medium with 18 pitchers/plant.

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“…This study showed that pitcher formation might be correlated with the gene expressed in the leaf. Thus, this study suggests that pitcher formation is part of leaf development [28].…”

Section: Resultsmentioning

confidence: 53%

Pitcher formation of Nepenthes ampullaria and Nepenthes rafflesiana on modified in vitro media

Isnaini,

Novitasari

2023

IOP Conf. Ser.: Earth Environ. Sci.

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Ontogeny and Anatomy of the Dimorphic Pitchers of Nepenthes rafflesiana Jack

Schwallier

Wely

Baak

et al. 2020

Plants

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An enigmatic feature of tropical pitcher plants belonging to the genus Nepenthes is their dimorphic prey-capturing pitfall traps. In many species, the conspicuously shaped upper and lower pitchers grow from a swollen leaf tendril tip until finally opening as insect-alluring devices. Few have studied the ontogeny of these traps from an anatomical and quantitative morphological perspective. We investigated whether the anatomy and development of lower and upper type pitchers of N. rafflesiana differ or overlap in terms of 3D geometric morphology and microstructure progression and presence. We hypothesized that there is an overlap in the initial, but not all, developmental stages of the two pitcher types and that one pitcher type is suspended in development. We identified four important morphological changes of pitcher ontogeny and defined these as curvation, elongation, inflation and maturation phases. Pitcher length indicated progress through developmental phases, and we propose to use it as a tool for indication of developmental stage. Microstructure development coincided with the developmental phases defined. Additionally, we discovered a new anatomical feature of extrafloral nectariferous peristomal glands between the inner peristome ridges of upper and lower pitchers being hollow and analyze the chemistry of the sugars on the outside of these glands. Ontogenetic shape analysis indicated that upper and lower pitcher types develop with similar phase progression but have no directly overlapping morphology. This means that upper pitchers are not a derived state from lower pitchers. Independent developmental programs evolved to produce distinctly shaped upper and lower pitchers in Nepenthes, likely to exploit different food sources.

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Development of SCAR Markers for Species Identification of the Genus Nepenthes (Nepenthaceae)

Anuniwat¹,

Chaveerach²,

Tanee³

et al. 2009

Pakistan J. of Biological Sciences

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Nepenthes species in Thailand, namely N. mirabilis Druce, N. gracilis Korth., N. smilesii Hemsl., N. ampullaria Jack and N. kampotiana Lecomte, were collected for development of Sequence Characterized Amplified Region (SCAR) marker, a genotype identification tool. Forty Random Amplified Polymorphic DNA (RAPD) primers were screened and three successful primers produced different banding patterns including five candidate species-specific markers. The candidate markers were cloned and sequenced. The marker sequences are 602, 379, 420, 473 and 1017 bp for N. mirabilis, N. gracilis, N. smilesii, N. ampullaria and N. kampotiana, respectively. Then the sequences were used to design primers for development of a species-specific band being a SCAR marker, including Mir 1, Mir 2 and Mir 3 for N. mirabilis; Gra 1 and Gra 2 for N. gracilis; Smi 1, Smi 2 and Smi 3 for N. smilesii; Amp 1 and Amp 2 for N. ampullaria and Kam 1 and Kam 2 of N. kampotiana. The primers were evaluated with each other Nepenthes species. Finally, species-specific SCAR markers were successfully developed for N. gracilis, N. ampullaria and N. kampotiana. Application of these markers is feasible for identification of Nepenthes species in Thailand.

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Differential Expressed Protein in Developing Stages of Nepenthes gracilis Korth. Pitcher

Cited by 3 publications

References 9 publications

Pitcher formation of Nepenthes ampullaria and Nepenthes rafflesiana on modified in vitro media

Pitcher formation of Nepenthes ampullaria and Nepenthes rafflesiana on modified in vitro media

Ontogeny and Anatomy of the Dimorphic Pitchers of Nepenthes rafflesiana Jack

Development of SCAR Markers for Species Identification of the Genus Nepenthes (Nepenthaceae)

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