Differential expression after UV-B radiation and characterization of chalcone synthase from the Patagonian hairgrass Deschampsia antarctica

Cuadra, Pedro; Guajardo, Joselin; Carrasco-Orellana, Cristian; Stappung, Yazmina; Fajardo, Víctor; Herrera, Raúl

doi:10.1016/j.phytochem.2019.112179

Cited by 16 publications

(10 citation statements)

References 76 publications

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“…Flavonoids have antioxidant properties and it has been shown that their production as well as the expression of genes associated with their synthesis could consequently be increased under stress conditions 45 . A previous study indicated that the expression level of the CHS gene depends on the tissue and plant growth stage, and it is also affected by external stimuli such as UV rays, plant growth regulators, and drought 46 , 47 . Also, numerous reports have shown that SA increases the levels of some secondary metabolites, especially those involved in plant defense mechanisms 48 .…”

Section: Discussionmentioning

confidence: 99%

The isolation and expression analysis of cinnamate 4-hydroxylase and chalcone synthase genes of Scrophularia striata under different abiotic elicitors

Rostami

Fazeli²,

Hojati

2022

Sci Rep

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The phenylpropanoid pathway serves as a rich source of metabolites in plants, and it is considered as a starting point for the production of many other important compounds such as the flavonoids, flavonols, coumarins, and lignans. Scrophularia striata is a member of the Lamiaceae family with some biological activities similar to flavonoid compounds such as antioxidant, antibacterial, anti-inflammatory and analgesic activities. Cinnamate 4-hydroxylase (C4H) and Chalcone synthase (CHS) are key enzymes of the phenylpropanoid pathway, leading to the biosynthesis of several secondary metabolites. In this study, two S. striata CHS and C4H were isolated and then analyzed. The investigation of the expression of these genes was performed under the effects of three salicylic acid (SA), jasmonic acid (JA), and gibberellic acid (GA) at concentrations of 100 and 300 ppm with a completely randomized design at the transcript level using Real Time PCR method. These have different expression patterns at developmental stages. Moreover, these genes present different sensitivities to hormonal treatment. Considering the total results, it was found that the amount of expression of these genes during the reproductive phase is higher than that of the vegetative phase. Additionally, the treatment of 300 ppm SA in the reproductive phase is the most effective treatment on increasing the corresponding phenylpropanoid compounds. A correlation analysis was performed between the phenylpropanoid compounds content and both CHS and C4H expression values at different phenological development stages. The results indicate that the expression variations of both CHS and C4H are significantly related to the changes in total phenolic content. We believe that the isolation of CHS and C4H can be helpful in better understanding phenylpropanoid metabolis.

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Section: Discussionmentioning

confidence: 99%

The isolation and expression analysis of cinnamate 4-hydroxylase and chalcone synthase genes of Scrophularia striata under different abiotic elicitors

Rostami

Fazeli²,

Hojati

2022

Sci Rep

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“… 2017 ; Cuadra et al. 2020 ). The species used for tree construction were as follows Cephalopholis argus NC022142, Cephalopholis boenak NC021134, Cephalopholis leopardus NC065827, Cephalopholis miniata NC060350, Epinephelus aeneus LC545417, Epinephelus akaara EU043377, Epinephelus areolatus NC020785, Plectropomus areolatus NC021405, Plectropomus laevis NC057260, Pseudanthias dispar NC028286, Pseudanthias huchtii OP611574 and Acrossocheilis fasciatus NC023378.…”

Section: Methodsmentioning

confidence: 99%

The complete mitochondrial genome of pseudanthias pascalus (Jordan & Tanaka, 1927) (perciformes: serranidae)

Duan,

Zhao,

Gong

et al. 2024

Mitochondrial DNA Part B

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Pseudanthias pascalus (Jordan & Tanaka, 1927) (Perciformes: Serranidae) is a species of brightly colored saltwater fish found in tropical coastal reef communities. In this study, we reported the sequence of mitochondrial DNA from P. pascalus . The accession number is OP611422. The complete mitochondrial genome of P. pascalus was 16,863 bp in length, including 13 protein-coding genes (PCGs), 12S and 16S rRNAs, 22 tRNA genes, and one displacement loop (D-loop). Most PCGs had ATG-start codons and TAA-end codons. The A + T contents were 54.61%. Phylogenetic analysis showed that P. pascalus is most closely related to Pseudanthias huchtii . We sequenced the entire mitochondrial genome of P. pascalus , providing improved marker identification information for the classification of the family and species conservation. These data will be useful for relative ecological and phylogenetic studies.

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“…Among litter components, lignin has been identified as the most photoreactive due to its absorption in the UV and blue–green region of the solar spectrum [ 384 , 391 ]. However, recent studies have found that cellulose and hemicellulose are even more susceptible to photodegradation than lignin [ 393 , 394 ]. These discrepancies are an unresolved knowledge gap that could be addressed by identifying differences in the photodegradation action spectra for lignin, cellulose, and hemicellulose.…”

Section: Effects On Biogeochemical Cycles and Climate Feedbacksmentioning

confidence: 99%

Interactive effects of changes in UV radiation and climate on terrestrial ecosystems, biogeochemical cycles, and feedbacks to the climate system

Barnes

Robson

Zepp

et al. 2023

Photochem Photobiol Sci

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Terrestrial organisms and ecosystems are being exposed to new and rapidly changing combinations of solar UV radiation and other environmental factors because of ongoing changes in stratospheric ozone and climate. In this Quadrennial Assessment, we examine the interactive effects of changes in stratospheric ozone, UV radiation and climate on terrestrial ecosystems and biogeochemical cycles in the context of the Montreal Protocol. We specifically assess effects on terrestrial organisms, agriculture and food supply, biodiversity, ecosystem services and feedbacks to the climate system. Emphasis is placed on the role of extreme climate events in altering the exposure to UV radiation of organisms and ecosystems and the potential effects on biodiversity. We also address the responses of plants to increased temporal variability in solar UV radiation, the interactive effects of UV radiation and other climate change factors (e.g. drought, temperature) on crops, and the role of UV radiation in driving the breakdown of organic matter from dead plant material (i.e. litter) and biocides (pesticides and herbicides). Our assessment indicates that UV radiation and climate interact in various ways to affect the structure and function of terrestrial ecosystems, and that by protecting the ozone layer, the Montreal Protocol continues to play a vital role in maintaining healthy, diverse ecosystems on land that sustain life on Earth. Furthermore, the Montreal Protocol and its Kigali Amendment are mitigating some of the negative environmental consequences of climate change by limiting the emissions of greenhouse gases and protecting the carbon sequestration potential of vegetation and the terrestrial carbon pool. Graphical abstract

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Differential expression after UV-B radiation and characterization of chalcone synthase from the Patagonian hairgrass Deschampsia antarctica

Cited by 16 publications

References 76 publications

The isolation and expression analysis of cinnamate 4-hydroxylase and chalcone synthase genes of Scrophularia striata under different abiotic elicitors

The isolation and expression analysis of cinnamate 4-hydroxylase and chalcone synthase genes of Scrophularia striata under different abiotic elicitors

The complete mitochondrial genome of pseudanthias pascalus (Jordan & Tanaka, 1927) (perciformes: serranidae)

Interactive effects of changes in UV radiation and climate on terrestrial ecosystems, biogeochemical cycles, and feedbacks to the climate system

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