Differential Expression of a Set of Genes in Follicular and Classic Variants of Papillary Thyroid Carcinoma

İğci, Yusuf Ziya; Arslan, Ahmet; Akarsu, Ersin; Erkılıç, Suna; İğci, Mehri; Öztuzcu, Serdar; Cengiz, Beyhan; Göğebakan, Bülent; Çakmak, Ecir Ali; Demiryürek, Abdullah Tuncay

doi:10.1007/s12022-011-9157-8

Cited by 23 publications

(13 citation statements)

References 44 publications

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“…The NDUFC2 gene has higher mutation rates and has been recommended as a target candidate for treating colorectal carcinoma tumorigenesis 33 . The NDUFC2 gene also shows lower expression in papillary thyroid carcinoma patients 34 . Additionally, complex I deficiency is mainly caused by nuclear-encoded subunit gene mutations 35 , and our results showed that both the protein expression level and enzyme activity of complex I were decreased after irradiation.…”

Section: Discussionmentioning

confidence: 94%

Grape seed proanthocyanidins prevent irradiation-induced differentiation of human lung fibroblasts by ameliorating mitochondrial dysfunction

Yang

Liu

Chen

et al. 2017

Sci Rep

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Radiation-induced lung fibrosis (RILF) is a long-term adverse effect of curative radiotherapy. The accumulation of myofibroblasts in fibroblastic foci is a pivotal feature of RILF. In the study, we found the inhibitory effect of grape seed proanthocyanidins (GSPs) on irradiation-induced differentiation of human fetal lung fibroblasts (HFL1). To explore the mechanism by which GSPs inhibit fibroblast differentiation, we measured the reactive oxygen species (ROS) levels, mitochondrial function, mitochondrial dynamics, glycolysis and the signaling molecules involved in fibroblast transdifferentiation. GSPs significantly reduced the production of cellular and mitochondrial ROS after radiation. The increases in mitochondrial respiration, proton leak, mitochondrial ATP production, lactate release and glucose consumption that occurred in response to irradiation were ameliorated by GSPs. Furthermore, GSPs increased the activity of complex I and improved the mitochondrial dynamics, which were disturbed by irradiation. In addition, the elevation of phosphorylation of p38MAPK and Akt, and Nox4 expression induced by irradiation were attenuated by GSPs. Blocking Nox4 attenuated irradiation-mediated fibroblast differentiation. Taken together, these results indicate that GSPs have the ability to inhibit irradiation-induced fibroblast-to-myofibroblast differentiation by ameliorating mitochondrial dynamics and mitochondrial complex I activity, regulating mitochondrial ROS production, ATP production, lactate release, glucose consumption and thereby inhibiting p38MAPK-Akt-Nox4 pathway.

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Section: Discussionmentioning

confidence: 94%

Grape seed proanthocyanidins prevent irradiation-induced differentiation of human lung fibroblasts by ameliorating mitochondrial dysfunction

Yang

Liu

Chen

et al. 2017

Sci Rep

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“…BRAF K601E is very rare in FTC and was only described in one case in previous publications (6,7). In addition, the overall gene expression profile in FVPTC is distinct from classic PTC, underlining these differences (8,9). In summary, FVPTC shares features of classic PTC and FTC on the morphological, genetic, and prognostic level.…”

Section: Introductionmentioning

confidence: 90%

Comprehensive MicroRNA Expression Profiling Identifies Novel Markers in Follicular Variant of Papillary Thyroid Carcinoma

Dettmer

Perren²,

Moch³

et al. 2013

Thyroid

121

104

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Background: Follicular variant of papillary thyroid carcinoma (FVPTC) shares features of papillary (PTC) and follicular (FTC) thyroid carcinomas on a clinical, morphological, and genetic level. MicroRNA (miRNA) deregulation was extensively studied in PTCs and FTCs. However, very limited information is available for FVPTC. The aim of this study was to assess miRNA expression in FVPTC with the most comprehensive miRNA array panel and to correlate it with the clinicopathological data. Methods: Forty-four papillary thyroid carcinomas (17 FVPTC, 27 classic PTC) and eight normal thyroid tissue samples were analyzed for expression of 748 miRNAs using Human Microarray Assays on the ABI 7900 platform (Life Technologies, Carlsbad, CA). In addition, an independent set of 61 tumor and normal samples was studied for expression of novel miRNA markers detected in this study. Results: Overall, the miRNA expression profile demonstrated similar trends between FVPTC and classic PTC. Fourteen miRNAs were deregulated in FVPTC with a fold change of more than five (up/down), including miRNAs known to be upregulated in PTC (miR-146b-3p, -146-5p, -221, -222 and miR-222-5p) and novel miRNAs (miR-375, -551b, 181-2-3p, 99b-3p). However, the levels of miRNA expression were different between these tumor types and some miRNAs were uniquely dysregulated in FVPTC allowing separation of these tumors on the unsupervised hierarchical clustering analysis. Upregulation of novel miR-375 was confirmed in a large independent set of follicular cell derived neoplasms and benign nodules and demonstrated specific upregulation for PTC. Two miRNAs (miR-181a-2-3p, miR-99b-3p) were associated with an adverse outcome in FVPTC patients by a Kaplan-Meier ( p < 0.05) and multivariate Cox regression analysis ( p < 0.05). Conclusions: Despite high similarity in miRNA expression between FVPTC and classic PTC, several miRNAs were uniquely expressed in each tumor type, supporting their histopathologic differences. Highly upregulated miRNA identified in this study (miR-375) can serve as a novel marker of papillary thyroid carcinoma, and miR181a-2-3p and miR-99b-3p can predict relapse-free survival in patients with FVPTC thus potentially providing important diagnostic and predictive value.

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“…A comparative study of MCM3 protein expression levels was also done in follicular variant of papillary thyroid carcinoma (FVPTC), classic variant of papillary thyroid carcinoma (CVPTC), and multi-nodular goitre (MNG) tissues. MCM3 protein expression was higher in FVPTC and CVPTC as compared to MNG (Igci et al, 2011;Igci et al, 2014). This shows that MCM3 might be used as a suitable marker in order to distinguish FVPTC and CVPTC from MNG.…”

Section: Papillary Thyroid Carcinomamentioning

confidence: 81%

MCM3 (minichromosome maintenance complex component 3)

Arman¹,

Bozgeyik²,

İğci³

2017

Atlas of Genetics and Cytogenetics in Oncology and Haematology

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Differential Expression of a Set of Genes in Follicular and Classic Variants of Papillary Thyroid Carcinoma

Cited by 23 publications

References 44 publications

Grape seed proanthocyanidins prevent irradiation-induced differentiation of human lung fibroblasts by ameliorating mitochondrial dysfunction

Grape seed proanthocyanidins prevent irradiation-induced differentiation of human lung fibroblasts by ameliorating mitochondrial dysfunction

Comprehensive MicroRNA Expression Profiling Identifies Novel Markers in Follicular Variant of Papillary Thyroid Carcinoma

MCM3 (minichromosome maintenance complex component 3)

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