Differential expression of epithelial cell adhesion molecule in salivary gland neoplasms

Phattarataratip, Ekarat; Masorn, Marisa; Jarupoonphol, W.; Supatthanayut, Sirinpaporn; Saeoweiang, Pichanee

doi:10.1016/j.anndiagpath.2016.05.007

Cited by 9 publications

(6 citation statements)

References 33 publications

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“…While media supplementation with calcium had no effect, calcium chelation with EDTA completely abrogated aggregation. Salivary gland acinar cells have both calcium dependent (via E-cadherin) and calcium independent (via epithelial cell adhesion molecule (EpCAM)) mechanisms for intercellular adhesion (Davis & Reynolds, 2006; Phattarataratip, Masorn, Jarupoonphol, Supatthanayut, & Saeoweiang, 2016). EDTA-mediated inhibition of aggregate formation may occur directly by blocking calcium dependent contacts between cells, similar to earlier studies showing that antibody inhibition of E-cadherin alters the organization of embryonic salivary gland tissues (Wei et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Salivary gland cell aggregates are derived from self-organization of acinar lineage cells

Varghese

Hansen

Sharipol

et al. 2019

Archives of Oral Biology

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Objective: The objective of this study was to characterize the mechanism by which salivary gland cells (SGC) aggregate in vitro. Design: Timelapse microscopy was utilized to analyze the process of salivary gland aggregate formation using both primary murine and human salivary gland cells. The role of cell density, proliferation, extracellular calcium, and secretory acinar cells in aggregate formation was investigated. Finally, the ability of cells isolated from irradiated glands to form aggregates was also evaluated. Results: Salivary gland cell self-organization rather than proliferation was the predominant mechanism of aggregate formation in both primary mouse and human salivary gland cultures (SGC). Aggregation was found to require extracellular calcium while acinar lineage cells account for ~80% of the total aggregate cell population. Finally, aggregation was not impaired by irradiation. Conclusions: The data reveal that aggregation occurs as a result of heterogeneous salivary gland cell self-organization rather than from stem cell proliferation and differentiation, contradicting previous dogma. These results suggest a re-evaluation of aggregate formation as a criterion defining salivary gland stem cells.

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Section: Discussionmentioning

confidence: 99%

Salivary gland cell aggregates are derived from self-organization of acinar lineage cells

Varghese

Hansen

Sharipol

et al. 2019

Archives of Oral Biology

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“…[25][26][27][28][29][30][31] Phattarataratip et al studied epithelial cell adhesion molecule expression in various salivary gland neoplasms, including mucoepidermoid carcinoma, adenoid cystic carcinoma, pleomorphic adenoma, and polymorphous low-grade adenocarcinoma. 39 They included EpCAM and showed different EpCAM expression patterns among salivary gland neoplasms. They demonstrated that decreased EpCAM expression was associated with aggressive features in mucoepidermoid carcinoma, and the ACCs showed negative or weakly positive immunoreactivity to EpCAM, contrary to our result.…”

Section: Discussionmentioning

confidence: 99%

Expression of EpCAM in adenoid cystic carcinoma

et al. 2018

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The mutational landscape of adenoid cystic carcinoma (ACC) is currently being revealed, but further studies are needed to identify biomarkers as therapeutic targets or prognostic factors of ACC. In this study, we investigated the expression of epithelial cell adhesion molecule (EpCAM) in ACCs. We retrospectively collected 83 cases of surgically resected ACCs. Using tissue microarray, we conducted immunohistochemical staining using the anti-EpCAM antibody. EpCAM expression was analysed by intensity score and the total immunostaining score. The positivity was 97.6% (81/83 cases), regardless of the intensity score. A higher histological grade (p = 0.006) and specific tumour location (non-salivary gland origin, p = 0.02) showed a correlation with higher EpCAM intensity. Higher EpCAM expression by total immunostaining score was associated with histological grade (p = 0.004), distant metastasis (p = 0.004) and poorer prognosis (overall survival p = 0.015 and progressionfree survival p = 0.033). We suggest EpCAM as a candidate prognostic marker and a putative therapeutic target in ACC. Also, ACCs arising from salivary gland and non-salivary gland sites, respectively, might display different pathophysiologies in which EpCAM could play a role.

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“…The immunohistochemical staining was performed with Leica Microsystems Bond-Max Autostainer System as previously described [ 29 ]. The 5-µm thick specimen sections were deparaffinized at 60 o C using 60-minute incubation with the Bond Dewax Solution.…”

Section: Methodsmentioning

confidence: 99%

Expression of SOX2 and OCT4 in odontogenic cysts and tumors

et al. 2021

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Background Aberrant expression of stem cell markers has been observed in several types of neoplasms. This trait attributes to the acquired stem-like property of tumor cells and can impact patient prognosis. The objective of this study was to comparatively analyze the expression and significance of SOX2 and OCT4 in various types of odontogenic cysts and tumors. Methods Fifty-five cases of odontogenic cysts and tumors, including 15 ameloblastomas (AM), 5 adenomatoid odontogenic tumors (AOT), 5 ameloblastic fibromas (AF), 5 calcifying odontogenic cysts (COC), 10 dentigerous cysts (DC) and 15 odontogenic keratocysts (OKC) were investigated for the expression of SOX2 and OCT4 immunohistochemically. Results Most OKCs (86.7 %) and all AFs expressed SOX2 in more than 50 % of epithelial cells. Its immunoreactivity was moderate-to-strong in all epithelial cell types in both lesions. In contrast, SOX2 expression was undetectable in AOTs and limited to the ameloblast-like cells in a minority of AM and COC cases. Most DCs showed positive staining in less than 25 % of cystic epithelium. Significantly greater SOX2 expression was noted in OKC compared with DC or AM, and in AF compared with COC or AOT. OCT4 rarely expressed in odontogenic lesions with the immunoreactivity being mild and present exclusively in OKCs. Conclusions SOX2 is differentially expressed in odontogenic cysts and tumors. This could be related to their diverse cells of origin or stages of histogenesis. The overexpression of SOX2 and OCT4 in OKC indicates the acquired stem-like property. Future studies should investigate whether the overexpression of OCT4 and SOX2 contributes to the aggressive behaviors of the tumors.

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Differential expression of epithelial cell adhesion molecule in salivary gland neoplasms

Cited by 9 publications

References 33 publications

Salivary gland cell aggregates are derived from self-organization of acinar lineage cells

Salivary gland cell aggregates are derived from self-organization of acinar lineage cells

Expression of EpCAM in adenoid cystic carcinoma

Expression of SOX2 and OCT4 in odontogenic cysts and tumors

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