Differential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells

Guzmán-Gutiérrez, Enrique; Sandoval, C.; Nova, E. de; Castillo, Juan Luis; Vera, Juan Carlos; Lamperti, Liliana; Krause, Bernardo J.; Salomón, Carlos; Sepúlveda, Catarina; Aguayo, Claudio; Sobrevía, Luis

doi:10.1016/j.placenta.2010.07.016

Cited by 15 publications

(25 citation statements)

References 37 publications

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“…15,16,21 Briefly, fresh human peripheral venous blood was collected from eight healthy female volunteers (25-30 y), non-smokers, not receiving any medication and without any clinical diagnosis. This protocol was conducted according to the protocols defined by the Declaration of Helsinki Ethic Committee and informed consent was signed by every volunteer participating in this study.…”

Section: Isolation and Culture Of Hepcmentioning

confidence: 99%

“…PCM was changed every 3 days and hEPC were selected as cells attached to fibronectin-coated plates. In addition, experiments were performed as described previously in hEPC cultured for 3 days (hEPC-3d) or 14 days (hEPC-14d) 15,16 after cell starving (12 h) using PCM containing 2% of serum without growth factors. CarboxyFluorescein conjugated KDR (R&D System Inc., Minneapolis, MN, USA) as previously described 15,16 Characterization of L-arginine transport L-arginine transport was measured as described.…”

Section: Isolation and Culture Of Hepcmentioning

confidence: 99%

“…In addition, experiments were performed as described previously in hEPC cultured for 3 days (hEPC-3d) or 14 days (hEPC-14d) 15,16 after cell starving (12 h) using PCM containing 2% of serum without growth factors. CarboxyFluorescein conjugated KDR (R&D System Inc., Minneapolis, MN, USA) as previously described 15,16 Characterization of L-arginine transport L-arginine transport was measured as described. 22 L-arginine transport (1 min, 37°C) was initiated by the addition of HEPES-buffered Krebs solution containing unlabelled L-arginine (0.1-500 µM) and L-[ 3 H]-arginine (4 µCi/ml) and was stopped by the addition of cold phosphate buffer.…”

Section: Isolation and Culture Of Hepcmentioning

confidence: 99%

“…15,23 Saturable L-arginine transport kinetic parameters V max and K m were calculated from data where the non-saturable, linear uptake of L-arginine was subtracted from transport data and fitted to the Michaelis-Menten hyperbola assuming a single saturable transport system.…”

Section: Characterization Of Hepc Differentiation Markers By Flow Cytmentioning

confidence: 99%

“…For example, on the 3 th day (hEPC-3d), hEPC are positive for CD34, CD133 and Oct4 markers, which represents an immature phenotype, while at day 14 th (hEPC-14d), hEPC lose the expression of those surface molecules and express membrane markers of mature endothelium, such as VEGFR-2. 15,16 Accordingly, hEPC exhibited a differential expression and activity of the equilibrative and concentrative nucleoside transporters, 15 showing that the cell differentiation affects the expression and function of membrane-associated transporters.…”

Section: Introductionmentioning

confidence: 99%

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L-arginine transport and nitric oxide synthesis in human endothelial progenitor cells

Díaz-Pérez

Radojkovic²,

Aguilera³

et al. 2012

Journal of Cardiovascular Pharmacology

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Section: Isolation and Culture Of Hepcmentioning

confidence: 99%

Section: Isolation and Culture Of Hepcmentioning

confidence: 99%

Section: Isolation and Culture Of Hepcmentioning

confidence: 99%

Section: Characterization Of Hepc Differentiation Markers By Flow Cytmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

L-arginine transport and nitric oxide synthesis in human endothelial progenitor cells

Díaz-Pérez

Radojkovic²,

Aguilera³

et al. 2012

Journal of Cardiovascular Pharmacology

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Adenosine receptors: Modulators of lipid availability that are controlled by lipid levels

Leiva

Guzmán-Gutiérrez

Contreras-Duarte

et al. 2017

Molecular Aspects of Medicine

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Adenosine as well as agonists and antagonists for the four adenosine receptor subtypes (A1R, A2AR, A2BR and A3R) play a role in several key physiological and pathophysiological processes, including the regulation of vascular tone, thrombosis, immune response, inflammation, and angiogenesis. This review focuses on the adenosine-mediated regulation of lipid availability in the cell and in the systemic circulation as well in humans and animal models. Therefore, adenosine, mainly by acting on A1R, inhibits lipolysis activity, leading to reduction of the circulating fatty acid levels. This nucleoside can also participate in the early development of atherosclerosis by inhibiting the formation of foam cells via stimulation of cholesterol efflux through A2AR expressed on macrophages and reduction of the inflammatory process by activating A2AR and A2BR. Adenosine also appears to modulate intracellular cholesterol availability in Niemann-Pick type C1 disease and Alzheimer disease via A2AR and A3, respectively. Remarkably, the role of adenosine receptors in the regulation of plasma total cholesterol and triglyceride levels has been studied in animal models. Thus, an anti-atherogenic role for A2BR as well as a pro-atherogenic role of A2AR and A1 have been proposed; A3R has not been shown to participate in the control of lipid levels or the development of atherosclerosis. Surprisingly, and despite the role of A2A in the inhibition of foam cell formation among isolated cells, this receptor appears to be pro-atherogenic in mice. Remarkably, the role of adenosine receptors in human dyslipidaemia and atherosclerosis must to be elucidated. Additionally, it has been reported that increased lipid levels impair the effects of adenosine/adenosine receptors in controlling vascular tone, and we speculate on the possibility that this impairment could be due to alterations in the composition of the membrane microdomains where the adenosine receptors are located. Finally, a possible role for adenosine/adenosine receptors in the phenomena of dyslipidaemia in pregnancy has been proposed.

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Apolipoprotein A-I enhances proliferation of human endothelial progenitor cells and promotes angiogenesis through the cell surface ATP synthase

González-Pecchi

Valdés

Pons

et al. 2015

Microvascular Research

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Differential expression of functional nucleoside transporters in non-differentiated and differentiated human endothelial progenitor cells

Cited by 15 publications

References 37 publications

L-arginine transport and nitric oxide synthesis in human endothelial progenitor cells

L-arginine transport and nitric oxide synthesis in human endothelial progenitor cells

Adenosine receptors: Modulators of lipid availability that are controlled by lipid levels

Apolipoprotein A-I enhances proliferation of human endothelial progenitor cells and promotes angiogenesis through the cell surface ATP synthase

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