Differential expression of genes associated with lipid metabolism in longissimus dorsi of Korean bulls and steers

Bong, Jin Jong; Jeong, Jin Young; Rajasekar, Periyannan; Cho, Young Moo; Kwon, Eung Gi; Kim, Hyeong Cheol; Paek, Bong Hyun; Baik, Myunggi

doi:10.1016/j.meatsci.2012.02.004

Cited by 65 publications

(69 citation statements)

References 33 publications

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“…IMF deposition is influenced by several factors, such as breed, genotype, gender, and nutrition (Maltin et al, 2003). In addition, castration increases IMF deposition in Korean cattle (Bong et al, 2012), resulting in improved beef quality. Large variation in IMF content remains in Korean cattle steers, which suggests that beef quality can be further improved.…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphisms of the FATP1 gene and their associations with meat quality traits in Chinese Qinchuan cattle

Wei

et al. 2015

Genet. Mol. Res.

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Section: Introductionmentioning

confidence: 99%

Genetic polymorphisms of the FATP1 gene and their associations with meat quality traits in Chinese Qinchuan cattle

Wei

et al. 2015

Genet. Mol. Res.

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“…In this study, we used steer LM tissue samples from previous work (Bong et al, 2012). Slaughter age was 846±30 days, and carcass weight was 398±10 kg.…”

Section: Methodsmentioning

confidence: 99%

“…Slaughter age was 846±30 days, and carcass weight was 398±10 kg. We separated the muscle and IMF portion from the intact LM tissues to determine tissue-specific DNA methylation pattern, as previously described (Bong et al, 2012). …”

Section: Methodsmentioning

confidence: 99%

Association of DNA Methylation Levels with Tissue-specific Expression of Adipogenic and Lipogenic Genes in Longissimus dorsi Muscle of Korean Cattle

Baik

Piao

et al. 2014

Asian Australas. J. Anim. Sci

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Epigenetic factors, such as DNA methylation status, may regulate adipogenesis and lipogenesis, thus affecting intramuscular fat (IMF) deposition in longissimus dorsi muscle (LM) of beef cattle. In Korean cattle steers, the LM consists mainly of muscle tissue. However, the LM tissue also contains IMF. We compared the gene expression levels between the IMF and muscle portions of the LM after tissue separation. Real-time polymerase chain reaction analysis showed that the mRNA levels of both adipogenic peroxisome proliferator-activated receptor gamma isoform 1 (PPARG1) and lipogenic fatty acid binding protein 4 (FABP4) were higher (p<0.01) in the IMF than in the muscle portion of the LM. We determined DNA methylation levels of regulatory regions of the PPARG1 and FABP4 genes by pyrosequencing of genomic DNA. DNA methylation levels of two of three CpG sites in the PPARG1 gene promoter region were lower (p<0.05) in the IMF than in the muscle portion of the LM. DNA methylation levels of all five CpG sites from the FABP4 gene promoter region were also lower (p<0.001) in the IMF than in the muscle portion. Thus, mRNA levels of both PPARG1 and FABP4 genes were inversely correlated with DNA methylation levels in regulatory regions of CpG sites of the corresponding gene. Our findings suggest that DNA methylation status regulates tissue-specific expression of adipogenic and lipogenic genes in the IMF and muscle portions of LM tissue in Korean cattle.

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“…This would require a great amount of energy which proceeds from glucose but also from other energy substrates such as BHB (Boisclair et al, 1993;Herdt, 2003) or TG (Hocquette and Bauchart, 1999), and therefore there would be an uptake from blood at a fast rate. Moreover, the gender might have affected TG metabolism, as Bong et al (2012) reported that castration increases lipogenic gene expression of both acetyl-CoA carboxylase and fatty acid synthase while downregulating lipolytic gene expression of both adipose triglyceride lipase and monoglyceride lipase in the Longissimus muscle.…”

Section: Blood Metabolitesmentioning

confidence: 99%

Performance and Carcass Quality of Forage-Fed Steers as an Alternative to Concentrate-Based Beef Production

Alibés

Joy

Albertı́

et al. 2014

Italian Journal of Animal Science

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Differential expression of genes associated with lipid metabolism in longissimus dorsi of Korean bulls and steers

Cited by 65 publications

References 33 publications

Genetic polymorphisms of the FATP1 gene and their associations with meat quality traits in Chinese Qinchuan cattle

Genetic polymorphisms of the FATP1 gene and their associations with meat quality traits in Chinese Qinchuan cattle

Association of DNA Methylation Levels with Tissue-specific Expression of Adipogenic and Lipogenic Genes in Longissimus dorsi Muscle of Korean Cattle

Performance and Carcass Quality of Forage-Fed Steers as an Alternative to Concentrate-Based Beef Production

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