Differential expression of genes encoding phosphate transporters contributes to arsenic tolerance and accumulation in shrub willow (Salix spp.)

Puckett, Emily E.; Serapiglia, Michelle J.; DeLeon, Alyssa; Long, Stephanie; Minocha, Rakesh; Smart, Lawrence B.

doi:10.1016/j.envexpbot.2011.07.008

Cited by 45 publications

(16 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, arsenic‐tolerant Holcus lanatus (Meharg & Macnair, ) and shrub willow ( Salix spp.) (Puckett et al ., ) genotypes exhibit lower Pi/AsV uptake capacity as compared with sensitive genotypes. This decreased AsV uptake capacity can lead to enhanced arsenic accumulation over time (Hartley‐Whitaker et al ., ; Puckett et al ., ).…”

Section: Introductionmentioning

confidence: 96%

“…(Puckett et al ., ) genotypes exhibit lower Pi/AsV uptake capacity as compared with sensitive genotypes. This decreased AsV uptake capacity can lead to enhanced arsenic accumulation over time (Hartley‐Whitaker et al ., ; Puckett et al ., ). Similarly, a semidominant mutation in the Arabidopsis AtPht1;1 Pi transporter, which slows the uptake of both Pi and AsV, was found to confer AsV tolerance and enhanced arsenic accumulation (Catarecha et al ., ).…”

Section: Introductionmentioning

confidence: 96%

See 1 more Smart Citation

A member of the Phosphate transporter 1 (Pht1) family from the arsenic‐hyperaccumulating fern Pteris vittata is a high‐affinity arsenate transporter

et al. 2015

View full text Add to dashboard Cite

Summary Pteris vittata exhibits enhanced arsenic uptake, but the corresponding mechanisms are not well known. The prevalent form of arsenic in most soils is arsenate, which is a phosphate analog and a substrate for Phosphate transporter 1 (Pht1) transporters. Herein we identify and characterize three P. vittata Pht1 transporters. Pteris vittata Pht1 cDNAs were isolated and characterized via heterologous expression in Saccharomyces cerevisiae (yeast) and Nicotiana benthamiana leaves. Expression of the PvPht1 loci in P. vittata gametophytes was also examined in response to phosphate deficiency and arsenate exposure. Expression of each of the PvPht1 cDNAs complemented the phosphate uptake defect of a yeast mutant. Compared with yeast cells expressing Arabidopsis thaliana Pht1;5, cells expressing PvPht1;3 were more sensitive to arsenate, and accumulated more arsenic. Uptake assays with yeast cells and radiolabeled 32P revealed that PvPht1;3 and AtPht1;5 have similar affinities for phosphate, but the affinity of PvPht1;3 for arsenate is much greater. In P. vittata gametophytes, PvPht1;3 transcript levels increased in response to phosphate (Pi) deficiency and arsenate exposure. PvPht1;3 is induced by Pi deficiency and arsenate, and encodes a phosphate transporter that has a high affinity for arsenate. PvPht1;3 probably contributes to the enhanced arsenate uptake capacity and affinity exhibited by P. vittata.

show abstract

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 96%

A member of the Phosphate transporter 1 (Pht1) family from the arsenic‐hyperaccumulating fern Pteris vittata is a high‐affinity arsenate transporter

et al. 2015

View full text Add to dashboard Cite

show abstract

“…To normalize the qPCR measurements the reference genes Actin and Ubiquitin were used, with published primers designed from closely related species [15–16] (S1 Table). These primers were then tested on a number of different cDNA samples from S .…”

Section: Methodsmentioning

confidence: 99%

Identification of a Differentially Expressed TIR-NBS-LRR Gene in a Major QTL Associated to Leaf Rust Resistance in Salix

et al. 2016

View full text Add to dashboard Cite

An earlier identified major quantitative trait locus for resistance towards the willow leaf rust fungus Melampsora larici-epitea in a Salix viminalis x (S. viminalis × S. schwerinii) population was used to identify potential resistance genes to the rust pathogen. Screening a genomic bacterial artificial chromosome library with markers from the peak position of the QTL region revealed one gene with TIR-NBS-LRR (Toll Interleukin1 Receptor-Nucleotide Binding Site-Leucine-Rich Repeat) domain structure indicative of a resistance gene. The resistance gene analog was denoted RGA1 and further analysis revealed a number of non-synonymous single nucleotide polymorphisms in the LRR domain between the resistant and susceptible Salix genotypes. Gene expression levels under controlled conditions showed a significantly lower constitutive expression of RGA1 in the susceptible genotype. In addition, the susceptible genotype showed a significantly reduced expression level of the RGA1 gene at 24 hours post inoculation with M. larici-epitea. This indicates that the pathogen may actively suppress RGA1 gene expression allowing a compatible plant-pathogen interaction and causing infection.

show abstract

“…For example, Cd competition with macro-and micronutrients such as P, calcium (Ca), magnesium (Mg), iron (Fe), manganese (Mn), Cu, Zn, and nickel (Ni) for the same transmembrane carrier proteins has been reported (Rivetta et al, 1997). Because it is a chemical analogue, As may compete with P for uptake sites and exert its toxicity in plants by interfering with the many physiological functions performed by the nutrient (Meharg and Hartley-Whitaker, 2002;Puckett et al, 2012).…”

Section: Trace Elements and Seedling Establishmentmentioning

confidence: 99%

Trace Elements Tolerance Modulated by Antioxidant System in Plants

Gomes¹,

Carneiro²,

Garcia³

2014

Oxidative Damage to Plants

View full text Add to dashboard Cite

Differential expression of genes encoding phosphate transporters contributes to arsenic tolerance and accumulation in shrub willow (Salix spp.)

Cited by 45 publications

References 45 publications

A member of the Phosphate transporter 1 (Pht1) family from the arsenic‐hyperaccumulating fern Pteris vittata is a high‐affinity arsenate transporter

A member of the Phosphate transporter 1 (Pht1) family from the arsenic‐hyperaccumulating fern Pteris vittata is a high‐affinity arsenate transporter

Identification of a Differentially Expressed TIR-NBS-LRR Gene in a Major QTL Associated to Leaf Rust Resistance in Salix

Trace Elements Tolerance Modulated by Antioxidant System in Plants

Contact Info

Product

Resources

About