Differential expression of heat shock proteins and heat stress transcription factor genes in rice exposed to different levels of heat stress

Chandel, Girish; Dubey, Monika; Meena, Rakesh Kumar

doi:10.1007/s13562-012-0156-8

Cited by 23 publications

(16 citation statements)

References 37 publications

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“…The rice genotype RRF-127 showed the highest up-regulation of 14.3 Fold increase followed by Annada (13.9 Fold), Karma mashuri (11.5 Fold), and CGZR-1 (3.7 Fold). Previous studies reported that OsHSP 26.7 gene belongs to sHSP (small heat shock proteins) class of genes and showed strong up-regulation under heat stress conditions in comparison to control (Chandel et al, 2013). OsHSP16.9 gene, in the present investigation has shown up-regulation in almost all rice genotypes under heat stress conditions.…”

Section: Expression Pattern Of High Temperature Responsive Genes In Rsupporting

confidence: 53%

“…Annada has shown highest up-regulation of 1.9 Fold increase followed by CGZR-1 (1.5 Fold), Nagina -22 (1.4 Fold), and Karma mashuri (1.3 Fold). 60Kda-chaperon was also reported to be up-regulated by minimal levels under heat stress but it is lately induced under stress (Chandel et al, 2013).Small heat shock proteins (sHSPs) are the largest ubiquitous HSP subgroup whose molecular weight ranges from 12 t0 42 KDa.Small HSPs sequence analysis indicates that members belonging to this family include evolutionarily divergent N-terminal part, which is followed by a conserved α-crystallin domain and a short C-terminal tail (De Jong et al, 1993). It's been recognized that ten separate sHSPs families have been conserved in both monocots and dicot plants, which indicates the diversity for sHSPs mechanisms (Scharf et al, 2001).…”

Section: Expression Pattern Of High Temperature Responsive Genes In Rmentioning

confidence: 99%

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Characterization of Physiological Responses and Deciphering Differential Expression of Heat stress Responsive Candidate Genes in Rice under High Temperature

Lohitha¹,

H²,

Chandel³

2019

IJBSM

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With increasing global temperature (by 1.5-4.8 °C by 2100) and its negative impact on crop productivity of major food crops including rice (by 41% by 21 st century), a study was undertaken to assess the genes involved in maintaining crop metabolism and sustenance under high temperature. Rice, most important staple food crop feeds around 3 billion people, hence producing improved heat tolerant varieties is necessary. High temperatures produce new group proteins called 'Heat Shock Proteins (HSPs), whose transcription is guarded by heat shock transcription factors (Hsfs). HSPs refold proteins maintaining functional conformation, aiding in host-defence mechanisms. The aim of this research was to assess physiological and biochemical changes and to analyze key genes expressed due to high temperature exposure. Expression profiling of five heat responsive genes (OsHSP26.7, OsHSP16.9, OsHSP-DnaJ, OsHSP18 and 60Kda-chaperon), in rice showed up-regulation. Pollen fertility, spikelet fertility, photosynthetic pigments like chlorophyll a, chlorophyll b and total chlorophyll content decreased under heat stress while membrane stability index, proline and Malondialdehyde was increased. This study suggested OsHSP26.7 as most responsive gene under stress and rice genotypes RRF-127, Annada with heat tolerant adaptive mechanisms and better performance under high temperatures. These findings were observed to be in correlation with the phenological, biochemical and expression analysis studies carried out with five different heat responsive genes. This can be taken as a base for heat tolerance response of rice crop, which may be useful for further validation studies of the candidate genes responsive for heat stress in rice.

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Section: Expression Pattern Of High Temperature Responsive Genes In Rsupporting

confidence: 53%

Section: Expression Pattern Of High Temperature Responsive Genes In Rmentioning

confidence: 99%

Characterization of Physiological Responses and Deciphering Differential Expression of Heat stress Responsive Candidate Genes in Rice under High Temperature

Lohitha¹,

H²,

Chandel³

2019

IJBSM

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“…At the other end of the spectrum of genetic analyses, using proteomic approach Jagadish et al [ 34 ] reported much higher accumulation of small heat shock proteins (sHSP) in N22 variety compared with the heat-sensitive variety Moroberekan, and the moderately heat-tolerant variety IR64, when submitted to high temperature during anthesis. Likewise, semi-quantitative RT-PCR based gene expression analysis, targeting heat shock proteins (Hsps) and heat shock transcription factors (Hsfs), identified N22 as the variety with the highest level of expression for most of the Hsps and Hsfs genes tested [ 35 ]. Several other studies have analyzed differential gene expression in rice subjected to high temperature [ 36 , 37 , 38 ] but they did not focus on the reproductive stage and the resulting spikelet sterility.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide association analysis for heat tolerance at flowering detected a large set of genes involved in adaptation to thermal and other stresses

et al. 2017

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Fertilization sensitivity to heat in rice is a major issue within climate change scenarios in the tropics. A panel of 167 indica landraces and improved varieties was phenotyped for spikelet sterility (SPKST) under 38°C during anthesis and for several secondary traits potentially affecting panicle micro-climate and thus the fertilization process. The panel was genotyped with an average density of one marker per 29 kb using genotyping by sequencing. Genome-wide association analyses (GWAS) were conducted using three methods based on single marker regression, haplotype regression and simultaneous fitting of all markers, respectively. Fourteen loci significantly associated with SPKST under at least two GWAS methods were detected. A large number of associations was also detected for the secondary traits. Analysis of co-localization of SPKST associated loci with QTLs detected in progenies of bi-parental crosses reported in the literature allowed to narrow -down the position of eight of those QTLs, including the most documented one, qHTSF4.1. Gene families underlying loci associated with SPKST corresponded to functions ranging from sensing abiotic stresses and regulating plant response, such as wall-associated kinases and heat shock proteins, to cell division and gametophyte development. Analysis of diversity at the vicinity of loci associated with SPKST within the rice three thousand genomes, revealed widespread distribution of the favourable alleles across O. sativa genetic groups. However, few accessions assembled the favourable alleles at all loci. Effective donors included the heat tolerant variety N22 and some Indian and Taiwanese varieties. These results provide a basis for breeding for heat tolerance during anthesis and for functional validation of major loci governing this trait.

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“…Essa classe de proteínas normalmente está envolvida na resposta a altas temperaturas e a outros estresses (Chandel et al, 2013).…”

Section: Resultsunclassified

Análise de associação quanto à produtividade e seus caracteres componentes em linhagens e cultivares de arroz de terras altas

Mendes

Borba

Bueno

et al. 2014

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi identificar, por meio da análise de mapeamento associativo, os marcadores moleculares relacionados à produtividade do arroz de terras altas e aos seus caracteres componentes. Foram usadas 113 linhagens e cultivares de arroz de terras altas, da Coleção Nuclear de Arroz da Embrapa, com reduzido vínculo genético entre si. Os seguintes caracteres componentes da produtividade foram avaliados: número de panículas por metro, número de grãos por panícula e peso de 100 grãos. Dos 115 marcadores utilizados, 25 (21,7%) associaram-se significativamente a um ou mais caracteres. Entre os 29 SSR ("simple sequence repeats") colocalizados em QTL ("quantitative trait loci") de produtividade de arroz, 12 foram associados aos caracteres avaliados e considerados como candidatos para uso na seleção assistida por marcadores. Os marcadores NP914540, Q6ZGD1 e Q69JE3, associados ao número de grãos por panícula, ainda não foram anotados no arroz e podem constituir o ponto de partida para estudos de genômica funcional. Entre os marcadores derivados de sequências transcritas, NP914526 e NP914533 destacam-se por pertencer a rotas metabólicas relacionadas ao aumento do potencial produtivo de arroz.Termos para indexação: Oryza sativa, coleção nuclear, desequilíbrio de ligação, genômica funcional, mapeamento associativo, seleção assistida por marcadores. Association analysis for yield and its component traits in lines and cultivars of upland riceAbstract -The objective of this work was to identify, through analysis of associative mapping, the molecular markers related to upland rice yield and its component traits. One hundred thirteen lines and cultivars of upland rice, with reduced admixture, from the Rice Core Collection of Embrapa, were used. The following yield component traits were evaluated: number of panicles per meter, number of grains per panicle, and weight of 100 grains. Out of the 115 used markers, 25 (21.7%) were significantly associated with one or more traits. Among the 29 SSR (simple sequence repeats) co-located in yield QTL (quantitative trait loci) in rice, 12 were associated with the evaluated traits and considered candidates for use in marker-assisted selection. The markers NP914540, Q6ZGD1, and Q69JE3, associated with the number of grains per panicle, are not yet annotated in rice and should constitute the starting point for functional genomics studies. Among the markers derived from transcribed sequences, NP914526 and NP914533 stand out for belonging to metabolic pathways related to increased yield potential of rice.

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Differential expression of heat shock proteins and heat stress transcription factor genes in rice exposed to different levels of heat stress

Cited by 23 publications

References 37 publications

Characterization of Physiological Responses and Deciphering Differential Expression of Heat stress Responsive Candidate Genes in Rice under High Temperature

Characterization of Physiological Responses and Deciphering Differential Expression of Heat stress Responsive Candidate Genes in Rice under High Temperature

Genome-wide association analysis for heat tolerance at flowering detected a large set of genes involved in adaptation to thermal and other stresses

Análise de associação quanto à produtividade e seus caracteres componentes em linhagens e cultivares de arroz de terras altas

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