Differential expression of selected candidate genes in bovine embryos produced in vitro and cultured with chemicals modulating lipid metabolism

Ghanem, Nasser; Ha, A-Na; Fakruzzaman, Md.; Bang, Jin-Young; Lee, Sang-Chan; Kong, Il‐Keun

doi:10.1016/j.theriogenology.2014.03.024

Cited by 51 publications

(46 citation statements)

References 57 publications

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“…The results showed that the relative abundance of CPT2 mRNA was increased in embryos cultured in the presence of L-carnitine and PES when compared with the control group (GHANEM et al, 2014).…”

Section: Genetic Markers Related To Lipid Metabolismmentioning

confidence: 92%

“…In a study by Ghanem et al (2014), the regulatory genes of these lipid droplet-forming enzymes were down-regulated in embryos supplemented with L-carnitine and PES compared with the control group. This result demonstrated that these genes are promising candidates as biomarkers of lipid content in bovine IVP embryos.…”

Section: Acylmentioning

confidence: 97%

“…Some studies have shown that the metabolic profile is different in these two types of cells, and trophectoderm consumes high levels of pyruvate and produces lactate, whereas ICM consumes more glucose (GOPICHANDRAN;LEESE, 2003). In human blastocysts, it was demonstrated that oxygen consumption in ICM and ET was identical (YAMANAKA et al, 2011).…”

Section: Embryonic Energy Metabolismmentioning

confidence: 99%

“…For example, in bovine embryos produced in vivo, less amino acid is produced than in IVP embryos (STURMEY et al, 2010 (GUERIF et al, 2013).…”

Section: Embryonic Energy Metabolismmentioning

confidence: 99%

“…Thus, the evaluation of the expression of genes related to lipid metabolism has been performed to predict the ability of embryos to respond to different treatments during maturation and in vitro culture, such as the use of substances that can generate positive responses to the resistance of IVP embryos to the cryopreservation process (TAKAHASHI et al, 2013;BATISTA et al, 2014;GHANEM et al, 2014;BALDOCEDA et al, 2015). Among these studies, the CPT2 gene was cited for generating carnitine palmitoyltransferase 2 formation, which is essential for the FA oxidation that occurs inside the mitochondria.…”

Section: Genetic Markers Related To Lipid Metabolismmentioning

confidence: 99%

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Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant?

Dias

Pivato

Dode

2017

SCA

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For the development of in vitro produced (IVP) as well as in vivo produced bovine embryos, it is extremely important that their energy metabolism works properly because the embryo must be able to metabolize energy substrates that are necessary for producing energy. Lipids play an important role in early embryonic development, acting as source of energy for oocytes and embryos. However, it is known that oocytes and embryos, mainly IVP, accumulate large amounts of lipids in the cytoplasm. Although they are extremely important in embryonic development, lipids have been associated with the reduced survival of bovine embryos following cryopreservation. There is evidence that at least four different categories of lipids affect embryo survival after cryopreservation, including triglycerides (TAG), free fatty acids, cholesterol and phospholipids. Thus, many studies are being conducted to improve the resistance of IVP embryos to the cryopreservation process by reducing the concentration or removing the source of serum from the medium or by reducing oocyte/embryo lipids using mechanical or chemical means. Regarding the use of delipidating agents that reduce the uptake and synthesis of fatty acids (FA) by cells, substances such as phenazine ethosulfate (PES), forskolin, L-carnitine and isomers of conjugated linoleic acid (CLA) have been utilized. This review aims to address important issues related to embryonic energy metabolism, the importance of lipid metabolism and its relation to the cryopreservation of IVP bovine embryos by summarizing the latest research in this field. Key words: Bovine embryos. Cryopreservation. Energy metabolism. Lipids. ResumoPara o desenvolvimento de embriões bovinos produzidos in vitro (PIV) e in vivo, é extremamente importante que o metabolismo energético funcione de maneira adequada, pois o embrião precisa ser capaz de metabolizar os substratos energéticos necessários para a produção de energia. Os lipídios desempenham papel importante no desenvolvimento embrionário inicial, atuando como fonte de energia para oócitos e embriões. Entretanto, sabe-se que oócitos e embriões bovinos, principalmente os PIV, acumulam grande quantidade de lipídios no citoplasma. Apesar de serem extremamente importantes no desenvolvimento embrionário, os lipídios têm sido associados com a reduzida sobrevivência após a criopreservação de embriões bovinos. Existem evidências de que pelo menos quatro classes de lipídios afetam a sobrevivência embrionária pós-criopreservação, sendo os triglicerídeos (TAG), ácidos graxos livres, colesterol e os fosfolipídios. Sendo assim, vários estudos estão sendo realizados com o intuito de melhorar a resistência dos embriões PIV ao processo de criopreservação, realizando a redução ou retirada do soro do meio ou a redução mecânica ou química de lipídios. Com relação ao uso de agentes delipidantes que diminuam a captação e síntese de ácidos graxos (AG) pelas células, substâncias como fenazina etossulfato (PES), forskolin, L-carnitina e isômeros do ácido linoleico conjugado (CLA) t...

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Section: Genetic Markers Related To Lipid Metabolismmentioning

confidence: 92%

Section: Acylmentioning

confidence: 97%

Section: Embryonic Energy Metabolismmentioning

confidence: 99%

“…For example, in bovine embryos produced in vivo, less amino acid is produced than in IVP embryos (STURMEY et al, 2010 (GUERIF et al, 2013).…”

Section: Embryonic Energy Metabolismmentioning

confidence: 99%

Section: Genetic Markers Related To Lipid Metabolismmentioning

confidence: 99%

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Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant?

Dias

Pivato

Dode

2017

SCA

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Early cleavages influence the molecular and the metabolic pattern of individually cultured bovine blastocysts

Milazzotto

Goissis

Chitwood

et al. 2016

Molecular Reproduction Devel

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Embryo morphokinetics suggests that the timing of the first embryonic cell divisions may predict the developmental potential of an embryo; however, correlations between embryonic morphokinetics and physiology are not clear. Here, we used RNA sequencing to determine the gene expression profile of in vitro-produced early- and late-dividing bovine embryos and their respective blastocysts, and compared these profiles to in vivo-produced blastocysts to identify differentially expressed genes (DEGs). Principal component analysis revealed that fast- and slow-dividing embryos possess similar transcript abundance over the first cleavages. By the blastocyst stage, however, more DEGs were observed between the fast- and slow-dividing embryo groups, whereas blastocysts from the slow-dividing group were more similar to in vivo-produced blastocysts. Gene ontology enrichment analysis showed that the slow-dividing and in vivo-produced blastocysts shared biological processes related to groups of up- or down-regulated genes when compared to the fast-dividing blastocysts. Based on these DEG results, we characterized the relationship between developmental kinetics and energy metabolism of in vitro-produced bovine embryos. Embryos from fast- and slow-dividing groups exhibited different pyruvate and lactate metabolism at 22 hr post-in vitro culture (hpc), glucose consumption at 96 hpc, and glutamate metabolism at 168 hpc. Glycogen storage was similar between cleavage-stage and morulae groups, but was higher in the blastocysts of the slow-dividing group. On the other hand, blastocysts of the fast-dividing group had a higher concentration of lipids. Taken together, these data identify transcriptomic and metabolic differences between embryos with different morphokinetics, suggesting that sorting embryos based on cleavage speed may select for different metabolic patterns. Mol. Reprod. Dev. 83: 324-336, 2016. © 2016 Wiley Periodicals, Inc.

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Expression profile of viability and stress response genes as a result of resveratrol supplementation in vitrified and in vitro produced cattle embryos

Saber,

Ibrahim,

Mahmoud

et al. 2024

Mol Biol Rep

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Differential expression of selected candidate genes in bovine embryos produced in vitro and cultured with chemicals modulating lipid metabolism

Cited by 51 publications

References 57 publications

Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant?

Change in energy metabolism of in vitro produced embryos: an alternative to make them more cryoresistant?

Early cleavages influence the molecular and the metabolic pattern of individually cultured bovine blastocysts

Expression profile of viability and stress response genes as a result of resveratrol supplementation in vitrified and in vitro produced cattle embryos

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