Differential expression of the LGI and SLIT families of genes in human cancer cells

Inhibition of medulloblastoma cell invasion by Slit

“…Rossi et al (2005) obtained similar results using RT-PCR analysis of Slit2 expression in the cerebral tumors and glioma cell lines. Although Slit2-Robo1 mRNA can be detected in many human tumors, this does not necessarily correlate with Slit2 protein content or activity.…”

Ultraviolet B‐induced LGI3 secretion protects human keratinocytes

“…LGI3 accumulates in the brain of aged monkeys and is involved in amyloid peptide uptake by astrocytes (4). Another study suggests that LGI3 is the most abundantly expressed protein in melanoma cell lines (5). While the critical roles of LGI3 in brain are quite well investigated, the expressions and physiological roles of LGI3 in skin cells are totally unknown.…”

The Tumor Suppressor Function of LGI1

“…Immunohistochemistry and mRNA analysis showed that LGI1 levels are very low or completely absent in most tumor tissues and in several cancer cell lines including, www.intechopen.com glioblastoma, neuroblastoma, melanoma and breast, furthermore the decrease of LGI1 expression shows a significant correlation with the grade of malignancy of astrocytic gliomas (Rossi et al, 2005;Besleaga et al, 2003). DNA microarrays, SAGE and EST number suggest low or absent expression in the majority of tumor tissues (see Gabellini, 2007).…”

“…The typical alterations of chromoseme 10 observed in glioblastomas are not present in neuroblastoma; instead loss of the chromosome 1p region is frequent in neuroblastomas, as well as the amplification of the proto-oncogene MYCN and alterations of neurotrophin receptors levels (see Schwab, 2004). However, the results of RT-PCR analysis with a series of neuroblastoma cell lines suggested that LGI1 expression was either absent or very weak in the vast majority of the cases, suggesting that LGI1 gene is often downregulated in neuroblastoma cells as well (Rossi et al, 2005). Our results of RT-PCR analysis with two human neuroblastoma cell lines (SH-SY5Y and SK-NBE) employed for transfection experiments described below showed negligible levels of LGI1, especially when compared with expression in human whole brain, which was estimated about 30-fold greater.…”