Differential expression of three ABA-insensitive five binding protein (AFP)-like genes in wheat

Abstract

Abscisic acid (ABA) signaling includes positive and negative regulators in the signaling pathway. ABA-insensitive five (ABI5) binding protein (AtAFP), one of the negative regulators found in Arabidopsis, is involved in the proteolysis of a positive regulator, ABI5 (bZIP-type transcription factor). Three wheat orthologs (TaAFPs) of AtAFP were isolated. TaAFPs have a nuclear localization domain in the middle of the deduced amino acid sequence and an ABI5 binding domain in the C-terminal region as AtAFP. Three TaAFPs were located on the short arms of chromosomes 2A, 2B, and 2D of wheat, and based on their chromosomal locations, they were named TaAFP-A, TaAFP-B, and TaAFP-D. In comparison to AtAFP, which was activated in developing seeds and the early stage of germination, TaAFPs were expressed in a greater variety of tissues, such as flag leaves, roots, and leaves of seedlings, and developing grains. TaAFP-B was expressed predominantly in all tissues examined; TaAFP-A and TaAFP-D responded to ABA and stresses, such as salt and dehydration. These three TaAFPs may differentiate their roles in ABA signaling during wheat evolution.

Molecular and phylogenetic characterization of the homoeologous EPSP Synthase genes of allohexaploid wheat, Triticum aestivum (L.)

“…PCR amplification of genomic DNA from nulli-tetrasomic Chinese Spring wheat lines was used to determine the chromosome location and to confirm primer specificity during the cDNA and genomic cloning of TaEPSPS-7A1 , TaEPSPS-4A1 , and TaEPSPS-7D1 (see Methods ) [ 16 ] . For mapping, PCR amplification of nulli-tetrasomic genomic DNA was performed using gene-specific primers (Fig.…”

Molecular Characterization, Gene Evolution, and Expression Analysis of the Fructose-1, 6-bisphosphate Aldolase (FBA) Gene Family in Wheat (Triticum aestivum L.)

“…For instance, Nulli1A-Tetra1D (N1AT1D) lacks chromosome 1A (nullisomic-1A), but contains two chromosome pairs of chromosome 1D (tetrasomic-1D), resulting in a line containing no copies of chromosome 1A, two doses of chromosome 1B, and four doses of chromosome 1D. PCR analysis of genomic DNA from nulli-tetrasomic lines allows one to determine which chromosome gene is located on based on failure to amplify specific nullisomic lines (Ohnishi et al, 2008). PCR amplification was performed as follows: 94°C for 5 min; 94°C for 30 s, annealing at 60°C for 30 s, and extension at 72°C for 1 min, 35 cycles; and 72°C for 10 min for the final extension.…”

Identification of seed dormancy on chromosome 2BS from wheat cv. Chinese Spring

“…The highest frequency of QTL occurrence was detected in the chromosomes 3A, 3B, 3D, followed by QTLs on 4A, 4B, 4D Mares et al, 2002;Mohan et al, 2009;Anderson et al, 1993;Noda et al, 2002;Ohnishi et al, 2008). This group includes the second highest number of identified major and minor QphsR loci.…”

“…The major QphsR locus localized in 4AL was found in both white-and red-grained genotypes of various origins (Groos et al, 2002;Kato et al, 2001;Mares et al, 2005;Osa et al, 2003;Mori et al, 2005;Kulwal et al, 2004;Lohwasser et al, 2005;Torada et al, 2005;Chen et al, 2008). In the short arms of 2A, 2B and 2D, there are three identified genes (TaAFPs) involved in regulation of ABA-signaling-TaAFP-A, TaAFP-B and TaAFP-D, respectively (Ohnishi et al, 2008). Besides, some QTL loci were identified and mapped onsomes of the 1st group Flintham et al, 2002;Mohan et al, 2009;Knox et al, 2005), 5th group (Groos et al, 2002;Zanetti et al, 2000;Kulwal et al, 2004;Fofana et al, 2009) and 7th group (Zanetti et al, 2000;Miura et al, 2002;Mares et al, 2005;Rasul et al, 2009;Mohan et al, 2009) of homeologues among different wheat genotypes.…”